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学科主题药学
Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography
Chen, X. J.; Guo, B. L.; Li, S. P.; Zhang, Q. W.; Tu, P. F.; Wang, Y. T.
关键词flavonoids Epimedium HPLC pressurized liquid extraction
刊名JOURNAL OF CHROMATOGRAPHY A
2007-09-07
DOI10.1016/j.chroma.2007.06.020
1163期:1-2页:96-104
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]ELECTROKINETIC CAPILLARY CHROMATOGRAPHY ; DIODE-ARRAY DETECTION ; ZONE-ELECTROPHORESIS ; BREVICORNUM MAXIM ; AERIAL PARTS ; GLYCOSIDES ; KOREANUM ; ACTIVATION
英文摘要

Herba Epimedii (family Berberidaceae), Yinyanghuo in Chinese, is one of the commonly used Chinese medicines. Flavonoids are considered as its active components. In this study, a reliable pressurized liquid extraction (PLE) and HPLC method was developed for simultaneous determination of 15 flavonoids, namely hexandraside E, kaempferol-3-O-rhamnoside, hexandraside F, epimedin A, epimedin B, epimedin C, icariin, epimedoside C, baohuoside 11, caohuoside C, bachuoside VII, sagittatoside A, sagittatoside B, 2 ′′-O-rhamnosyl icariside II and baohuoside I in different species of Epimedium. The analysis was performed by using a Zorbax SB-C18 analytical column (250 min x 4.6 min I.D., 5 mu m) at gradient elution of water and acetonitrile with diode-array detection (270 nm). All calibration curves showed good linearity (r(2) > 0.9997) within test ranges. The LOD and LOQ were lower than 1.31 ng and 2.62 ng on column, respectively. The RSD for intra- and inter-day of 15 analytes was less than 3.8% at three levels, and the recoveries were 90.5-106.8%. The validated method was successfully applied for the analysis of 15 flavonoids in different species of Epimedium which had great variation on the contents of investigated flavonoids. Hierarchical clustering analysis based on the characteristics of 15 investigated compound peaks in HPLC profiles showed that 26 samples were divided into three main clusters, which were in accordance with their flavonoid contents. Four flavonoids including epimedin A, B, C and icariin were optimized as markers for quality control of the species of Epimedium used as Yinyanghuo. (C) 2007 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000249310800009
引用统计
被引频次:65[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65385
专题北京大学药学院_天然药物学系
作者单位1.Univ Macau, Inst Chinese Med Sci, Taipa, Macao SAR, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China
3.Chinese Acad Med Sci, Inst Med Plants Dev, Beijing 100094, Peoples R China
4.Peking Union Med Coll, Beijing 100094, Peoples R China
推荐引用方式
GB/T 7714
Chen, X. J.,Guo, B. L.,Li, S. P.,et al. Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2007,1163(1-2):96-104.
APA Chen, X. J.,Guo, B. L.,Li, S. P.,Zhang, Q. W.,Tu, P. F.,&Wang, Y. T..(2007).Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1163(1-2),96-104.
MLA Chen, X. J.,et al."Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1163.1-2(2007):96-104.
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