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学科主题: 药学
题名:
Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography
作者: Chen, X. J.; Guo, B. L.; Li, S. P.; Zhang, Q. W.; Tu, P. F.; Wang, Y. T.
关键词: flavonoids ; Epimedium ; HPLC ; pressurized liquid extraction
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2007-09-07
DOI: 10.1016/j.chroma.2007.06.020
卷: 1163, 期:1-2, 页:96-104
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: ELECTROKINETIC CAPILLARY CHROMATOGRAPHY ; DIODE-ARRAY DETECTION ; ZONE-ELECTROPHORESIS ; BREVICORNUM MAXIM ; AERIAL PARTS ; GLYCOSIDES ; KOREANUM ; ACTIVATION
英文摘要:

Herba Epimedii (family Berberidaceae), Yinyanghuo in Chinese, is one of the commonly used Chinese medicines. Flavonoids are considered as its active components. In this study, a reliable pressurized liquid extraction (PLE) and HPLC method was developed for simultaneous determination of 15 flavonoids, namely hexandraside E, kaempferol-3-O-rhamnoside, hexandraside F, epimedin A, epimedin B, epimedin C, icariin, epimedoside C, baohuoside 11, caohuoside C, bachuoside VII, sagittatoside A, sagittatoside B, 2 ′′-O-rhamnosyl icariside II and baohuoside I in different species of Epimedium. The analysis was performed by using a Zorbax SB-C18 analytical column (250 min x 4.6 min I.D., 5 mu m) at gradient elution of water and acetonitrile with diode-array detection (270 nm). All calibration curves showed good linearity (r(2) > 0.9997) within test ranges. The LOD and LOQ were lower than 1.31 ng and 2.62 ng on column, respectively. The RSD for intra- and inter-day of 15 analytes was less than 3.8% at three levels, and the recoveries were 90.5-106.8%. The validated method was successfully applied for the analysis of 15 flavonoids in different species of Epimedium which had great variation on the contents of investigated flavonoids. Hierarchical clustering analysis based on the characteristics of 15 investigated compound peaks in HPLC profiles showed that 26 samples were divided into three main clusters, which were in accordance with their flavonoid contents. Four flavonoids including epimedin A, B, C and icariin were optimized as markers for quality control of the species of Epimedium used as Yinyanghuo. (C) 2007 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000249310800009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65385
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.Univ Macau, Inst Chinese Med Sci, Taipa, Macao SAR, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China
3.Chinese Acad Med Sci, Inst Med Plants Dev, Beijing 100094, Peoples R China
4.Peking Union Med Coll, Beijing 100094, Peoples R China

Recommended Citation:
Chen, X. J.,Guo, B. L.,Li, S. P.,et al. Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2007,1163(1-2):96-104.
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