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学科主题: 临床医学
题名:
Augmentation of revascularization and prevention of plasma leakage by angiopoietin-1 and vascular endothelial growth factor co-transfection in rats with experimental limb ischaemia
作者: Jiang, J; Jiang, N; Gao, W; Zhu, JJ; Gu, YH; Shen, D; Chen, GH; Tang, J
关键词: angiopoietin-I ; vascular endothelial growth factor ; gene therapy ; angiogenesis
刊名: ACTA CARDIOLOGICA
发表日期: 2006-04-01
卷: 61, 期:2, 页:145-153
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cardiac & Cardiovascular Systems
研究领域[WOS]: Cardiovascular System & Cardiology
关键词[WOS]: INTRAMUSCULAR INJECTION ; TIE2 RECEPTOR ; IN-VITRO ; ANGIOGENESIS ; LIGAND ; EXPRESSION ; SECRETION ; APOPTOSIS ; SURVIVAL ; KINASE
英文摘要:

Background - Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) are endothelial cell-specific growth factors. In the current study, we tested the hypothesis that co-expression of AngI and VEGF could have an effect on development of leakage-resistant vessels.

Methods and results - Expression plasmids, pcD2 (control plasmid), pcD2/Ang1, pcD2/VEGF(121), or pcD2/AngI + pcD2/VEGF(121), were injected intramuscularly into an ischaemic hindlimb rat model, followed by electroporation. Collateral vessel development and skeletal muscle atrophy were assessed before and 7, 14, 28 days after treatment. Capillary density was significantly increased in the rats transfected with Ang1 or VEGF compared with that in the rats transfected with pcD2 alone (P < 0.05). Rats transfected with AngI + VEGF had the highest capillary density (P < 0.05). The mean perimeter ratio of ligated hindlimb to non-ligated hindlimb was lower with pcD2 treatment rats compared with that in the rats transfected with Ang1, VEGF or Ang1 +VEGF (P < 0.01). Limb necrosis was observed in two of 33 control rats, but none in the Ang1 and/or VEGF gene-transferred rats. The amount of extravasated Evans blue in rat ligated hindlimb muscle (7 days after treatment) treated with both Ang1 and VEGF was significantly less compared with that in the rats transfected with VEGF alone (P < 0.01).

Conclusions - The current study demonstrated that co-expression of AngI and VEGF genes in the ischaemic muscle effectively develops leakage-resistant vessels in the rat model. Therefore, this approach may provide a more appropriate therapeutic strategy in ischaemic vascular diseases.

语种: 英语
WOS记录号: WOS:000236967000004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65399
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 1, Dept Cardiol, Beijing 100083, Peoples R China
3.Peking Univ, Cardiovasc Res Inst, Beijing 100083, Peoples R China

Recommended Citation:
Jiang, J,Jiang, N,Gao, W,et al. Augmentation of revascularization and prevention of plasma leakage by angiopoietin-1 and vascular endothelial growth factor co-transfection in rats with experimental limb ischaemia[J]. ACTA CARDIOLOGICA,2006,61(2):145-153.
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