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学科主题临床医学
Augmentation of revascularization and prevention of plasma leakage by angiopoietin-1 and vascular endothelial growth factor co-transfection in rats with experimental limb ischaemia
Jiang, J; Jiang, N; Gao, W; Zhu, JJ; Gu, YH; Shen, D; Chen, GH; Tang, J
关键词angiopoietin-I vascular endothelial growth factor gene therapy angiogenesis
刊名ACTA CARDIOLOGICA
2006-04-01
61期:2页:145-153
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]INTRAMUSCULAR INJECTION ; TIE2 RECEPTOR ; IN-VITRO ; ANGIOGENESIS ; LIGAND ; EXPRESSION ; SECRETION ; APOPTOSIS ; SURVIVAL ; KINASE
英文摘要

Background - Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) are endothelial cell-specific growth factors. In the current study, we tested the hypothesis that co-expression of AngI and VEGF could have an effect on development of leakage-resistant vessels.

Methods and results - Expression plasmids, pcD2 (control plasmid), pcD2/Ang1, pcD2/VEGF(121), or pcD2/AngI + pcD2/VEGF(121), were injected intramuscularly into an ischaemic hindlimb rat model, followed by electroporation. Collateral vessel development and skeletal muscle atrophy were assessed before and 7, 14, 28 days after treatment. Capillary density was significantly increased in the rats transfected with Ang1 or VEGF compared with that in the rats transfected with pcD2 alone (P < 0.05). Rats transfected with AngI + VEGF had the highest capillary density (P < 0.05). The mean perimeter ratio of ligated hindlimb to non-ligated hindlimb was lower with pcD2 treatment rats compared with that in the rats transfected with Ang1, VEGF or Ang1 +VEGF (P < 0.01). Limb necrosis was observed in two of 33 control rats, but none in the Ang1 and/or VEGF gene-transferred rats. The amount of extravasated Evans blue in rat ligated hindlimb muscle (7 days after treatment) treated with both Ang1 and VEGF was significantly less compared with that in the rats transfected with VEGF alone (P < 0.01).

Conclusions - The current study demonstrated that co-expression of AngI and VEGF genes in the ischaemic muscle effectively develops leakage-resistant vessels in the rat model. Therefore, this approach may provide a more appropriate therapeutic strategy in ischaemic vascular diseases.

语种英语
WOS记录号WOS:000236967000004
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65399
专题北京大学第三临床医学院_心血管内科
作者单位1.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 1, Dept Cardiol, Beijing 100083, Peoples R China
3.Peking Univ, Cardiovasc Res Inst, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Jiang, J,Jiang, N,Gao, W,et al. Augmentation of revascularization and prevention of plasma leakage by angiopoietin-1 and vascular endothelial growth factor co-transfection in rats with experimental limb ischaemia[J]. ACTA CARDIOLOGICA,2006,61(2):145-153.
APA Jiang, J.,Jiang, N.,Gao, W.,Zhu, JJ.,Gu, YH.,...&Tang, J.(2006).Augmentation of revascularization and prevention of plasma leakage by angiopoietin-1 and vascular endothelial growth factor co-transfection in rats with experimental limb ischaemia.ACTA CARDIOLOGICA,61(2),145-153.
MLA Jiang, J,et al."Augmentation of revascularization and prevention of plasma leakage by angiopoietin-1 and vascular endothelial growth factor co-transfection in rats with experimental limb ischaemia".ACTA CARDIOLOGICA 61.2(2006):145-153.
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