学科主题临床医学
Antibodies Targeting Novel Neutralizing Epitopes of Hepatitis C Virus Glycoprotein Preclude Genotype 2 Virus Infection
Deng, Kai1; Liu, Ruyu2; Rao, Huiying1; Jiang, Dong3; Wang, Jianghua1; Xie, Xingwang1; Wei, Lai1
刊名PLOS ONE
2015-09-25
DOI10.1371/journal.pone.0138756
10期:9
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]HUMAN MONOCLONAL-ANTIBODIES ; E2 GLYCOPROTEIN ; IMMUNE-RESPONSES ; ENVELOPE ; PARTICLES ; IMMUNIZATION ; CHIMPANZEES ; PROTEIN ; SERUM
英文摘要

Currently, there is no effective vaccine to prevent hepatitis C virus (HCV) infection, partly due to our insufficient understanding of the virus glycoprotein immunology. Most neutralizing antibodies (nAbs) were identified using glycoprotein immunogens, such as recombinant E1E2, HCV pseudoparticles or cell culture derived HCV. However, the fact that in the HCV acute infection phase, only a small proportion of patients are self-resolved accompanied with the emergence of nAbs, indicates the limited immunogenicity of glycoprotein itself to induce effective antibodies against a highly evolved virus. Secondly, in previous reports, the immunogen sequence was mostly the genotype of the 1a H77 strain. Rarely, other genotypes/subtypes have been studied, although theoretically one genotype/subtype immunogen is able to induce cross-genotype neutralizing antibodies. To overcome these drawbacks and find potential novel neutralizing epitopes, 57 overlapping peptides encompassing the full-length glycoprotein E1E2 of subtype 1b were synthesized to immunize BALB/c mice, and the neutralizing reactive of the induced antisera against HCVpp genotypes 1-6 was determined. We defined a domain comprising amino acids (aa) 192-221, 232-251, 262-281 and 292-331 of E1, and 421-543, 564-583, 594-618 and 634-673 of E2, as the neutralizing regions of HCV glycoprotein. Peptides PUHI26 (aa 444-463) and PUHI45 (aa 604-618)-induced antisera displayed the most potent broad neutralizing reactive. Two monoclonal antibodies recognizing the PUHI26 and PUHI45 epitopes efficiently precluded genotype 2 viral (HCVcc JFH and J6 strains) infection, but they did not neutralize other genotypes. Our study mapped a neutralizing epitope region of HCV glycoprotein using a novel immunization strategy, and identified two monoclonal antibodies effective in preventing genotype 2 virus infection.

语种英语
WOS记录号WOS:000361800700088
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65403
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位1.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing Key Lab Hepatitis C & Immunotherapy Liver, Beijing 100871, Peoples R China
2.Capital Med Univ, Liver Dis Ctr, Beijing Ditan Hosp, Beijing, Peoples R China
3.Capital Med Univ, Inst Infect Dis, Beijing Ditan Hosp, Beijing Key Lab Emerging Infect Dis, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Deng, Kai,Liu, Ruyu,Rao, Huiying,et al. Antibodies Targeting Novel Neutralizing Epitopes of Hepatitis C Virus Glycoprotein Preclude Genotype 2 Virus Infection[J]. PLOS ONE,2015,10(9).
APA Deng, Kai.,Liu, Ruyu.,Rao, Huiying.,Jiang, Dong.,Wang, Jianghua.,...&Wei, Lai.(2015).Antibodies Targeting Novel Neutralizing Epitopes of Hepatitis C Virus Glycoprotein Preclude Genotype 2 Virus Infection.PLOS ONE,10(9).
MLA Deng, Kai,et al."Antibodies Targeting Novel Neutralizing Epitopes of Hepatitis C Virus Glycoprotein Preclude Genotype 2 Virus Infection".PLOS ONE 10.9(2015).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Deng, Kai]的文章
[Liu, Ruyu]的文章
[Rao, Huiying]的文章
百度学术
百度学术中相似的文章
[Deng, Kai]的文章
[Liu, Ruyu]的文章
[Rao, Huiying]的文章
必应学术
必应学术中相似的文章
[Deng, Kai]的文章
[Liu, Ruyu]的文章
[Rao, Huiying]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。