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学科主题: 临床医学
题名:
Cystathionine beta synthase participates in murine oocyte maturatione mediated by homocysteine
作者: Liang, Rong; Yu, Wei-Dong; Du, Jun-Bao; Yang, Li-Jun; Yang, Jing-Jing; Xu, Jian; Shang, Mei; Guo, Jing-Zhu
关键词: RNA interference ; small-interfering (si) RNA ; oocyte development ; granulosa cells ; cystathionine beta synthase (CBS) ; homocysteine
刊名: REPRODUCTIVE TOXICOLOGY
发表日期: 2007-07-01
DOI: 10.1016/j.reprotox.2007.04.002
卷: 24, 期:1, 页:89-96
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Reproductive Biology ; Toxicology
研究领域[WOS]: Reproductive Biology ; Toxicology
关键词[WOS]: RNA INTERFERENCE ; MAMMALIAN OOCYTES ; MOUSE OOCYTES ; EXPRESSION ; GENE ; MICE ; ANEUPLOIDY ; DEFECTS ; EMBRYOS ; GROWTH
英文摘要:

Our previous study demonstrated that cystathionine P synthase (CBS) is highly expressed in the cumulus-oocyte complex during ovulation. However, the role of CES during oocyte maturation remains uncertain. In this study, a small-interfering (si) RNA interference (siRNA) approach was used to investigate the potential role of CBS during oocyte maturation. Accompanied with a gradual increase of homocysteine, the introduction of CBS-siRNA into marine granulosa cells selectively depleted the corresponding target mRNA and protein for CBS as assessed by semi-quantitative reverse-transcriptase PCR (RT-PCR) and immuofluorescence staining. When fully grown, germinal vesicle (GV) oocytes matured in vitro for 16 h using medium from transfected granulosa cells, the functional suppression of CBS resulted in a significant increase in the rate of GV-arrested oocytes. The results of this study provide evidence that CBS participates in the process of oocyte maturation. Furthermore, this effect may be fulfilled by conditioning the level of homocysteine in the microenvironment of the oocyte. (c) 2007 Published by Elsevier Inc.

语种: 英语
WOS记录号: WOS:000248775200009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65410
Appears in Collections:北京大学第二临床医学院_儿科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Pediat, Beijing 100871, Peoples R China
2.Peking Univ, Peoples Hosp, Cent Lab, Beijing 100871, Peoples R China
3.Peking Univ, Hosp 1, Dept Pediat, Beijing 100871, Peoples R China
4.Peking Univ, Dept Histol & Embryol, Beijing 100871, Peoples R China

Recommended Citation:
Liang, Rong,Yu, Wei-Dong,Du, Jun-Bao,et al. Cystathionine beta synthase participates in murine oocyte maturatione mediated by homocysteine[J]. REPRODUCTIVE TOXICOLOGY,2007,24(1):89-96.
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