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Biotransformation of nodakenin and simultaneous quantification of nodakenin and its aglycone in incubated system of human intestinal bacteria by HPLC method
Zhang, Peng; Yang, Xiu-Wei
关键词nodakenin nodakenetin biotransformation human intestinal bacteria column liquid chromatography
刊名JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
2009
DOI10.1080/10286020902767716
11期:4页:371-379
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Applied ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]Plant Sciences ; Chemistry ; Pharmacology & Pharmacy
英文摘要

When nodakenin (1) was anaerobically incubated with human intestinal bacteria, nodakenetin (2) was found as a main biotransformed product. We developed a simple and selective reversed-phase high-performance liquid chromatographic method for simultaneous quantification of 1 and 2 in incubated system of human intestinal bacteria with 1. Chromatographic separation of 1 and 2 was performed on an analytical C(18) column, with a mobile phase of MeOH-H(2)O (4:6, v/v) at a flow rate of 1.0 ml/min and the UV detection was at 330 nm. The calibration curves were linear over the range of 0.15-24.0 mu g/ml for 1 and 0.7-13.2 mu g/ml for 2. The lower limits of detection and quantification were 0.01 and 0.1 mu g/ml for 1, and 0.005 and 0.05 mu g/ml for 2. The recoveries were (87.66 +/- 1.66), (79.89 +/- 2.53), and (82.96 +/- 5.61)% at 1.0, 2.0, and 8.0 mu g/ml, respectively, for 1 and (88.32 +/- 4.12), (78.15 +/- 4.39), and (76.22 +/- 3.29)% at 1.0, 4.0, and 16.0 mu g/ml, respectively, for 2. The intra-and interday precision and accuracy were validated by relative standard deviation, which were in the ranges of 1.25-4.16 and 2.16-6.12% for 1, and 1.98-6.45 and 2.56-4.57% for 2, respectively. This method has been applied to the simultaneous quantitation of 1 and 2 in incubated system of human intestinal bacteria with 1.

语种英语
WOS记录号WOS:000266720000013
项目编号30672609 ; 2002AA2Z343C ; 2004AA2Z3783 ; 2006BAI06A01-02 ; Z0004105040311
资助机构National Natural Science Foundation of China ; National High Technology Research and Development Program of China ; National Sciences and Technology Program of China ; Beijing Municipal Special-Purpose Science Foundation of China
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65413
专题北京大学药学院_天然药物学系
北京大学第一临床医学院_泌尿外科
作者单位Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
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GB/T 7714
Zhang, Peng,Yang, Xiu-Wei. Biotransformation of nodakenin and simultaneous quantification of nodakenin and its aglycone in incubated system of human intestinal bacteria by HPLC method[J]. JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH,2009,11(4):371-379.
APA Zhang, Peng,&Yang, Xiu-Wei.(2009).Biotransformation of nodakenin and simultaneous quantification of nodakenin and its aglycone in incubated system of human intestinal bacteria by HPLC method.JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH,11(4),371-379.
MLA Zhang, Peng,et al."Biotransformation of nodakenin and simultaneous quantification of nodakenin and its aglycone in incubated system of human intestinal bacteria by HPLC method".JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH 11.4(2009):371-379.
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