北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 天然药物学系  > 期刊论文
学科主题: 药学
题名:
Biotransformation of nodakenin and simultaneous quantification of nodakenin and its aglycone in incubated system of human intestinal bacteria by HPLC method
作者: Zhang, Peng; Yang, Xiu-Wei
关键词: nodakenin ; nodakenetin ; biotransformation ; human intestinal bacteria ; column liquid chromatography
刊名: JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
发表日期: 2009
DOI: 10.1080/10286020902767716
卷: 11, 期:4, 页:371-379
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Applied ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Chemistry ; Pharmacology & Pharmacy
英文摘要:

When nodakenin (1) was anaerobically incubated with human intestinal bacteria, nodakenetin (2) was found as a main biotransformed product. We developed a simple and selective reversed-phase high-performance liquid chromatographic method for simultaneous quantification of 1 and 2 in incubated system of human intestinal bacteria with 1. Chromatographic separation of 1 and 2 was performed on an analytical C(18) column, with a mobile phase of MeOH-H(2)O (4:6, v/v) at a flow rate of 1.0 ml/min and the UV detection was at 330 nm. The calibration curves were linear over the range of 0.15-24.0 mu g/ml for 1 and 0.7-13.2 mu g/ml for 2. The lower limits of detection and quantification were 0.01 and 0.1 mu g/ml for 1, and 0.005 and 0.05 mu g/ml for 2. The recoveries were (87.66 +/- 1.66), (79.89 +/- 2.53), and (82.96 +/- 5.61)% at 1.0, 2.0, and 8.0 mu g/ml, respectively, for 1 and (88.32 +/- 4.12), (78.15 +/- 4.39), and (76.22 +/- 3.29)% at 1.0, 4.0, and 16.0 mu g/ml, respectively, for 2. The intra-and interday precision and accuracy were validated by relative standard deviation, which were in the ranges of 1.25-4.16 and 2.16-6.12% for 1, and 1.98-6.45 and 2.56-4.57% for 2, respectively. This method has been applied to the simultaneous quantitation of 1 and 2 in incubated system of human intestinal bacteria with 1.

语种: 英语
所属项目编号: 30672609 ; 2002AA2Z343C ; 2004AA2Z3783 ; 2006BAI06A01-02 ; Z0004105040311
项目资助者: National Natural Science Foundation of China ; National High Technology Research and Development Program of China ; National Sciences and Technology Program of China ; Beijing Municipal Special-Purpose Science Foundation of China
WOS记录号: WOS:000266720000013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65413
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Peng,Yang, Xiu-Wei. Biotransformation of nodakenin and simultaneous quantification of nodakenin and its aglycone in incubated system of human intestinal bacteria by HPLC method[J]. JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH,2009,11(4):371-379.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Peng]'s Articles
[Yang, Xiu-Wei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Peng]‘s Articles
[Yang, Xiu-Wei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace