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Simultaneous determination of seven major diterpenoids in Pseudolarix kaempferi by high-performance liquid chromatography DAD method
Liu, Peng; Guo, Hui; Guo, Hongzhu; Sheng, Yuxin; Wang, Weixing; Xu, Man; Feng, Suxiu; Cheng, Fei; Guo, De-an
关键词RP-HPLC Pseudolarix kaempferi determination quality control Cleistocalyx operculatus
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2007-07-27
DOI10.1016/j.jpba.2007.03.005
44期:3页:730-736
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]TU-JIN-PI ; ACID-B ; CONSTITUENTS
英文摘要

A reversed phase high-performance liquid chromatography method was established for the first time to simultaneously qualify the seven major diterpenoids in Pseudolarix kaempferi, namely pseudolaric acid B O-beta-D-glucopyranoside (1), pseudolaric acid C-2 (2), pseudolaric acid C-1 (3), deacetylpseudolaric acid A (4), pseudolaric acid A O-beta-D-glueopyranoside (5), pseudolaric acid B (6) and pseudolaric acid A (7). The optimal conditions of separation and detection were achieved on an Inertsil ODS-3 column with gradient elution of methanol and 0.5% aqueous acetic acid (v/v) at the flow rate of 0.6 ml min(-1) within 40 min and detection wavelength set at 262 run. All calibration curves showed good linear regression (r(2) > 0.9999) within test ranges. This method provided good accuracy with recoveries in the range of 94.3-106.1% and good precision with R.S.D.s of repeatability and intermediate precision less than 0.57% and 4.67%, respectively. The method was successfully applied to qualitative and quantitative determination of 20 P. kaempferi among the 54 samples collected from different areas. The results revealed that the commercial crude drugs were seriously confused and the developed HPLC assay could be used as a suitable qualitative and quantitative determination method for P. kaempferi. (c) 2007 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000248738200014
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65416
专题北京大学药学院_天然药物学系
作者单位Peking Univ, Sch Pharmaceut Sci, Hlth Sci Ctr, Dept Nat Med, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Liu, Peng,Guo, Hui,Guo, Hongzhu,et al. Simultaneous determination of seven major diterpenoids in Pseudolarix kaempferi by high-performance liquid chromatography DAD method[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,44(3):730-736.
APA Liu, Peng.,Guo, Hui.,Guo, Hongzhu.,Sheng, Yuxin.,Wang, Weixing.,...&Guo, De-an.(2007).Simultaneous determination of seven major diterpenoids in Pseudolarix kaempferi by high-performance liquid chromatography DAD method.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,44(3),730-736.
MLA Liu, Peng,et al."Simultaneous determination of seven major diterpenoids in Pseudolarix kaempferi by high-performance liquid chromatography DAD method".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 44.3(2007):730-736.
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