北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 天然药物学系  > 期刊论文
学科主题: 药学
题名:
Simultaneous determination of seven major diterpenoids in Pseudolarix kaempferi by high-performance liquid chromatography DAD method
作者: Liu, Peng; Guo, Hui; Guo, Hongzhu; Sheng, Yuxin; Wang, Weixing; Xu, Man; Feng, Suxiu; Cheng, Fei; Guo, De-an
关键词: RP-HPLC ; Pseudolarix kaempferi ; determination ; quality control ; Cleistocalyx operculatus
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2007-07-27
DOI: 10.1016/j.jpba.2007.03.005
卷: 44, 期:3, 页:730-736
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: TU-JIN-PI ; ACID-B ; CONSTITUENTS
英文摘要:

A reversed phase high-performance liquid chromatography method was established for the first time to simultaneously qualify the seven major diterpenoids in Pseudolarix kaempferi, namely pseudolaric acid B O-beta-D-glucopyranoside (1), pseudolaric acid C-2 (2), pseudolaric acid C-1 (3), deacetylpseudolaric acid A (4), pseudolaric acid A O-beta-D-glueopyranoside (5), pseudolaric acid B (6) and pseudolaric acid A (7). The optimal conditions of separation and detection were achieved on an Inertsil ODS-3 column with gradient elution of methanol and 0.5% aqueous acetic acid (v/v) at the flow rate of 0.6 ml min(-1) within 40 min and detection wavelength set at 262 run. All calibration curves showed good linear regression (r(2) > 0.9999) within test ranges. This method provided good accuracy with recoveries in the range of 94.3-106.1% and good precision with R.S.D.s of repeatability and intermediate precision less than 0.57% and 4.67%, respectively. The method was successfully applied to qualitative and quantitative determination of 20 P. kaempferi among the 54 samples collected from different areas. The results revealed that the commercial crude drugs were seriously confused and the developed HPLC assay could be used as a suitable qualitative and quantitative determination method for P. kaempferi. (c) 2007 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000248738200014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65416
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Sch Pharmaceut Sci, Hlth Sci Ctr, Dept Nat Med, Beijing 100083, Peoples R China

Recommended Citation:
Liu, Peng,Guo, Hui,Guo, Hongzhu,et al. Simultaneous determination of seven major diterpenoids in Pseudolarix kaempferi by high-performance liquid chromatography DAD method[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,44(3):730-736.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Peng]'s Articles
[Guo, Hui]'s Articles
[Guo, Hongzhu]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Peng]‘s Articles
[Guo, Hui]‘s Articles
[Guo, Hongzhu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace