IR@PKUHSC  > 北京大学基础医学院  > 心血管所
学科主题基础医学
Quantification of liver fat in mice: comparing dual-echo Dixon imaging, chemical shift imaging, and 1H-MR spectroscopy
Peng, Xin-Gui1; Ju, Shenghong1; Qin, Yujiao1; Fang, Fang1; Cui, Xin2,3; Liu, George2,3; Ni, Yicheng4; Teng, Gao-Jun1
关键词non-alcoholic fatty liver disease obesity triglyceride spin-spin relaxation time T2 in vivo hydrogen magnetic resonance
刊名JOURNAL OF LIPID RESEARCH
2011-10-01
DOI10.1194/jlr.D016691
52期:10页:1847-1855
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]MAGNETIC-RESONANCE-SPECTROSCOPY ; BROWN ADIPOSE-TISSUE ; IN-VIVO ; HEPATIC STEATOSIS ; MR SPECTROSCOPY ; 4.7 TESLA ; DISEASE ; BIOPSY ; WATER ; LIPODYSTROPHIES
英文摘要

We evaluated dual-echo Dixon in-phase and out-of-phase (IP-OP), chemical shift imaging (CSI), and 1 H MRS (hydrogen MR spectroscopy) in estimating fat content (FC) in phantoms and in livers of mice. Phantoms were made according to the volume percentage of fat ranging from 0% to 100%. The three MR methods were performed to measure FC in phantoms and in livers of obese leptin-deficient (ob/ob), human BSCL2/seipin gene knockout (SKO), and wild-type (WT) mice. The results were compared with known FC in phantoms and to a reference standard from mice by histological semiautomatic vacuole segmentation (HIS-S) procedure and liver lipid (LL) chemical analysis. In phantoms, CSI underestimated FC from 50% to 100%, to a lesser extent than IP-OP. In vivo, liver FC in ob/ob and SKO mice measured by the three MR methods were all significantly higher than that in WT mice. Liver FC measured by IP-OP were significantly lower than that measured by CSI and MRS, with no significant difference between CSI and MRS. CSI and MRS showed a linear correlation with LL analysis and with each other. IP-OP underestimated FC, whereas CSI and MRS were more accurate for quantifying FC in both phantoms and liver. CSI and MRS have the potential to replace HIS-S and LL analysis in longitudinal studies.-Peng, X-G., S. Ju, Y. Qi, F. Fang, X. Cui, G. Liu, Y. Ni, and G-J. Teng. Quantification of liver fat in mice: comparing dual-echo Dixon imaging, chemical shift imaging, and 1H-MR spectroscopy. J. Lipid Res. 2011. 52: 1847-1855.

语种英语
WOS记录号WOS:000294727500008
项目编号30830039 ; 81071125 ; 2010CB933903
资助机构National Nature Science Foundation of China (NSFC) ; Major State Basic Research Development Program of China (973)
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65422
专题北京大学基础医学院_心血管所
作者单位1.Southeast Univ, Zhongda Hosp, Dept Radiol, Lab Mol Imaging, Nanjing 210009, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Minist Educ, Inst Cardiovasc Sci, Beijing 10091, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 10091, Peoples R China
4.Catholic Univ Louvain, Univ Hosp, Dept Radiol, B-3000 Louvain, Belgium
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Peng, Xin-Gui,Ju, Shenghong,Qin, Yujiao,et al. Quantification of liver fat in mice: comparing dual-echo Dixon imaging, chemical shift imaging, and 1H-MR spectroscopy[J]. JOURNAL OF LIPID RESEARCH,2011,52(10):1847-1855.
APA Peng, Xin-Gui.,Ju, Shenghong.,Qin, Yujiao.,Fang, Fang.,Cui, Xin.,...&Teng, Gao-Jun.(2011).Quantification of liver fat in mice: comparing dual-echo Dixon imaging, chemical shift imaging, and 1H-MR spectroscopy.JOURNAL OF LIPID RESEARCH,52(10),1847-1855.
MLA Peng, Xin-Gui,et al."Quantification of liver fat in mice: comparing dual-echo Dixon imaging, chemical shift imaging, and 1H-MR spectroscopy".JOURNAL OF LIPID RESEARCH 52.10(2011):1847-1855.
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