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Differential Expression of Plasma Proteins in Cyclosporine A-Induced Rat Acute Nephrotoxicity
Shu, Zhan1; Pu, Xiaoping2; Xiong, Xin1,3; Li, Quan2; Wang, Yue4; Zhai, Suodi1,3
关键词cyclosporine A acute nephrotoxicity rat plasma two dimensional gel electrophoresis
刊名BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
2009-03-01
DOI10.1271/bbb.80677
73期:3页:592-598
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology
资助者National Program for Key Basic Research Projects of China ; National Program for Key Basic Research Projects of China
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology
关键词[WOS]ALPHA-1-ACID GLYCOPROTEIN ; GEL-ELECTROPHORESIS ; CLUSTERIN ; KIDNEY ; SERUM ; TRANSPLANTATION ; CHROMATOGRAPHY ; INFLAMMATION ; GENTAMICIN ; INDUCTION
英文摘要

Cyclosporine A (CsA) is a effective and widely used immunosuppressive agent. Nevertheless, its intense nephrotoxicity restricts clinical application. In this study, wistar rats were randomly divided into a control group, a low-dose group and a high-dose group. The rats in the low-dose group and those in the high-dose group were given CsA at a daily dose of 5 mg per kg and 100 mg per kg by gavage for 7 d respectively, while the control group was given distilled water of equal volume. The extent of renal impairment was evaluated by renal function assay, and urease and pathological observation. The results showed that the rats in the low-dose group did not suffer remarkable renal impairment, while the rats in the high-dose group did. Two-dimensional gel electrophoresis (2DE) was utilized to resolve the plasma protein profile. Six spots showing stable and significantly different expression were identified by MALDI-TOF-MS and ESI-TOF-MS/MS respectively, the two induced proteins were identified respectively as alpha-1-acid glycoprotein (AAG) and clusterin (CLUS), and the one inhibited was haptoglobin (Hp).

语种英语
所属项目编号2004CB519802
资助者National Program for Key Basic Research Projects of China ; National Program for Key Basic Research Projects of China
WOS记录号WOS:000264904900020
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65429
专题北京大学第三临床医学院_药剂科
作者单位1.Peking Univ, Third Hosp, Dept Pharm, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China
3.Peking Univ, Therapeut Drug Monitoring & Clin Toxicol Ctr, Beijing 100191, Peoples R China
4.Peking Univ, Third Hosp, Dept Nephrol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Shu, Zhan,Pu, Xiaoping,Xiong, Xin,et al. Differential Expression of Plasma Proteins in Cyclosporine A-Induced Rat Acute Nephrotoxicity[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2009,73(3):592-598.
APA Shu, Zhan,Pu, Xiaoping,Xiong, Xin,Li, Quan,Wang, Yue,&Zhai, Suodi.(2009).Differential Expression of Plasma Proteins in Cyclosporine A-Induced Rat Acute Nephrotoxicity.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,73(3),592-598.
MLA Shu, Zhan,et al."Differential Expression of Plasma Proteins in Cyclosporine A-Induced Rat Acute Nephrotoxicity".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 73.3(2009):592-598.
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