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学科主题: 药学
题名:
Effects of chronic morphine treatment on protein expression in rat dorsal root ganglia
作者: Li, Quan2; Zhao, Xin2; Zhong, Li-Jun3; Yang, Hai-Yu2; Wang, Qi2; Pu, Xiao-Ping1,2
关键词: Two-dimensional electrophoresis ; Aldolase C ; Dorsal root ganglia (DRG) ; Morphine ; Proteasome subunit alpha type 3
刊名: EUROPEAN JOURNAL OF PHARMACOLOGY
发表日期: 2009-06-10
DOI: 10.1016/j.ejphar.2009.03.049
卷: 612, 期:1-3, 页:21-28
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: PROTEASOME CORE PARTICLE ; PROTEOMIC ANALYSIS ; NUCLEUS-ACCUMBENS ; GENE-EXPRESSION ; OPIOID RECEPTORS ; SH-SY5Y CELLS ; SPINAL-CORD ; IN-VIVO ; WITHDRAWAL ; EXPOSURE
英文摘要:

The initial aim of this Study was to identify protein changes in the dorsal root ganglia (DRG) associated with long-term morphine treatment. We carried out a differential proteomics analysis on samples from the DRG of control and morphine-dependent rats (5-40 mg/kg, subcutaneously, twice daily for 28 days) after 4 days of morphine withdrawal. Proteins showing a statistically significant variation between the two groups were selected for identification by mass spectrometric analysis. Twelve proteins were unambiguously identified, with the majority being enzymes involved in energy metabolism and protein degradation. signaling and cytoskeletal proteins. Aldolase C and proteasome subunit alpha type 3 (PRC8 or alpha 7) were further examined by Western blot analysis in the DRG, prefrontal cortex, nucleus accumbens, striatum, hippocampus, ventral tegmental area and locus coerules. In addition, expression of PRC8 in the nucleus accumbens tissue after spontaneous or naloxone-precipitated withdrawal was determined using immunohistochemical staining. Our results indicate that the expression levels of aldolase C and PRCS proteins were altered in a time- and region-specific manner, and suggest that they could be implicated in the molecular changes underlying adaptations Of neurons observed with chronic morphine treatment. (C) 2009 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2004CB518902
项目资助者: National Program for Key Basic Research ; Peking University Program for Drug Development 211 Project
WOS记录号: WOS:000267150900004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65432
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Dept Mol & Cellular Pharmacol, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
3.Peking Univ, Med & Pharmaceut Anal Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Li, Quan,Zhao, Xin,Zhong, Li-Jun,et al. Effects of chronic morphine treatment on protein expression in rat dorsal root ganglia[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2009,612(1-3):21-28.
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