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Effects of chronic morphine treatment on protein expression in rat dorsal root ganglia
Li, Quan2; Zhao, Xin2; Zhong, Li-Jun3; Yang, Hai-Yu2; Wang, Qi2; Pu, Xiao-Ping1,2
关键词Two-dimensional electrophoresis Aldolase C Dorsal root ganglia (DRG) Morphine Proteasome subunit alpha type 3
刊名EUROPEAN JOURNAL OF PHARMACOLOGY
2009-06-10
DOI10.1016/j.ejphar.2009.03.049
612期:1-3页:21-28
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]PROTEASOME CORE PARTICLE ; PROTEOMIC ANALYSIS ; NUCLEUS-ACCUMBENS ; GENE-EXPRESSION ; OPIOID RECEPTORS ; SH-SY5Y CELLS ; SPINAL-CORD ; IN-VIVO ; WITHDRAWAL ; EXPOSURE
英文摘要

The initial aim of this Study was to identify protein changes in the dorsal root ganglia (DRG) associated with long-term morphine treatment. We carried out a differential proteomics analysis on samples from the DRG of control and morphine-dependent rats (5-40 mg/kg, subcutaneously, twice daily for 28 days) after 4 days of morphine withdrawal. Proteins showing a statistically significant variation between the two groups were selected for identification by mass spectrometric analysis. Twelve proteins were unambiguously identified, with the majority being enzymes involved in energy metabolism and protein degradation. signaling and cytoskeletal proteins. Aldolase C and proteasome subunit alpha type 3 (PRC8 or alpha 7) were further examined by Western blot analysis in the DRG, prefrontal cortex, nucleus accumbens, striatum, hippocampus, ventral tegmental area and locus coerules. In addition, expression of PRC8 in the nucleus accumbens tissue after spontaneous or naloxone-precipitated withdrawal was determined using immunohistochemical staining. Our results indicate that the expression levels of aldolase C and PRCS proteins were altered in a time- and region-specific manner, and suggest that they could be implicated in the molecular changes underlying adaptations Of neurons observed with chronic morphine treatment. (C) 2009 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000267150900004
项目编号2004CB518902
资助机构National Program for Key Basic Research ; Peking University Program for Drug Development 211 Project
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65432
专题北京大学药学院
北京大学药学院_分子与细胞药理学系
北京大学医药卫生分析中心_研发办公室
作者单位1.Peking Univ, Dept Mol & Cellular Pharmacol, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
3.Peking Univ, Med & Pharmaceut Anal Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Li, Quan,Zhao, Xin,Zhong, Li-Jun,et al. Effects of chronic morphine treatment on protein expression in rat dorsal root ganglia[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2009,612(1-3):21-28.
APA Li, Quan,Zhao, Xin,Zhong, Li-Jun,Yang, Hai-Yu,Wang, Qi,&Pu, Xiao-Ping.(2009).Effects of chronic morphine treatment on protein expression in rat dorsal root ganglia.EUROPEAN JOURNAL OF PHARMACOLOGY,612(1-3),21-28.
MLA Li, Quan,et al."Effects of chronic morphine treatment on protein expression in rat dorsal root ganglia".EUROPEAN JOURNAL OF PHARMACOLOGY 612.1-3(2009):21-28.
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