IR@PKUHSC  > 北京大学第三临床医学院  > 耳鼻喉科
学科主题临床医学
In Vivo Electrochemical Monitoring of the Change of Cochlear Perilymph Ascorbate during Salicylate-Induced Tinnitus
Liu, Junxiu1; Yu, Ping2; Lin, Yuqing2; Zhou, Na1; Li, Tao1; Ma, Furong1; Mao, Lanqun2
刊名ANALYTICAL CHEMISTRY
2012-06-19
DOI10.1021/ac301087v
84期:12页:5433-5438
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]INNER HAIR CELL ; HEARING-LOSS ; BLOOD-FLOW ; CARBON NANOTUBES ; SCALA TYMPANI ; RAT STRIATUM ; GLUTAMATE ; ISCHEMIA ; BRAIN ; THERAPY
英文摘要

As one of the most important neurochemicals in biological systems, ascorbate plays vital roles in many physiological and pathological processes. In order to understand the roles of ascorbate in the pathological process of tinnitus, this study demonstrates an in vivo method for real time monitoring of the changes of ascorbate level in the cochlear perilymph of guinea pigs during the acute period of tinnitus induced by local microinfusion of salicylate with carbon fiber microelectrodes (CFMEs) modified with multiwalled carbon nanotubes (MWNTs). To accomplish in vivo electrochemical monitoring of ascorbate in the microenvironment of the cochlear perilymph, the MWNT-modified CFME is used as working electrode, a microsized Ag/AgCl is used as reference electrode, and Pt wire is used as counter electrode. Three electrodes are combined together around a capillary to form integrated capillary-electrodes. The integrated capillary-electrode is carefully implanted into the cochlear perilymph of guinea pigs and used both for externally microinfusing of salicylate into the cochlear perilymph and for real time monitoring of the change of ascorbate levels. The in vivo voltammetric method based on the integrated capillary-electrodes possesses a high selectivity and a good linearity for ascorbate determination in the cochlear perilymph of guinea pigs. With such a method, the basal level of cochlear perilymph ascorbate is determined to be 45.0 +/- 5.1 mu M (n = 6). The microinfusion of 10 mM salicylate (1 mu L/min, 5 min) into the cochlear decreases the ascorbate level to 28 +/- 10% of the basal level (n = 6) with a statistical significance (P < 0.05), implying that the decrease in ascorbate level in the cochlear may be associated with salicylate-induced tinnitus. This study essentially offers a new method for in vivo monitoring of the cochlear perilymph ascorbate following the salicylate-induced tinnitus and can thus be useful for investigation on chemical essences involved in tinnitus.

语种英语
WOS记录号WOS:000305320500040
项目编号20975104 ; 20935005 ; 21127901 ; 91132708 ; 20905071 ; 2010CB33502 ; 7112144 ; 7082100 ; KJCX2-YW-W25 ; Y2010015
资助机构NSF of China ; National Basic Research Program of China (973 Program) ; Beijing Science Foundation of China ; Chinese Academy of Sciences
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65434
专题北京大学第三临床医学院_耳鼻喉科
作者单位1.Peking Univ, Hosp 3, Dept Otorhinolaryngol, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Liu, Junxiu,Yu, Ping,Lin, Yuqing,et al. In Vivo Electrochemical Monitoring of the Change of Cochlear Perilymph Ascorbate during Salicylate-Induced Tinnitus[J]. ANALYTICAL CHEMISTRY,2012,84(12):5433-5438.
APA Liu, Junxiu.,Yu, Ping.,Lin, Yuqing.,Zhou, Na.,Li, Tao.,...&Mao, Lanqun.(2012).In Vivo Electrochemical Monitoring of the Change of Cochlear Perilymph Ascorbate during Salicylate-Induced Tinnitus.ANALYTICAL CHEMISTRY,84(12),5433-5438.
MLA Liu, Junxiu,et al."In Vivo Electrochemical Monitoring of the Change of Cochlear Perilymph Ascorbate during Salicylate-Induced Tinnitus".ANALYTICAL CHEMISTRY 84.12(2012):5433-5438.
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