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学科主题: 临床医学
题名:
Murine antibody against E2 can capture hepatitis C virus in vitro
作者: Ma, WB; Feng, BF; Tao, QM
关键词: hepatitis C virus ; neutralizing antibody ; envelop protein
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2001-08-01
卷: 114, 期:8, 页:860-863
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: HYPERVARIABLE REGION-1 ; HYPERIMMUNE SERUM ; INFECTION
英文摘要:

Objective To find neutralizing antibody candidates against hepatitis C virus (HCV) infection.

Methods We constructed two eukaryotic expression vectors which contained the E1 and E2 gene of HCV, and detected their expression in mammalian cells with transient expression. BALB/c mice were given subculaneous injections of constructed vectors combined with the IL-2 gene intraepidermally and evaluated for induced humoral immune responses by enzyme-linked immunosorbent assay (ELISA). We used an antibody-virus interaction assay to analyze the interaction of the antisera and HCV viral particles in vitro.

Results Anti E1 and anti-E2 antisera were obtained from immunized mice. The serum of mice immunized with the E2 gene immunoprecipitated the HCV isolate in source serum and reacted with the isolates unrelated to the original one.

Conclusions Anti-E2 antibody in induced mice can cross-reactively capture HCV particles, highlighting the possibility of generating broadly reactive anti-E2 antibodies.

语种: 英语
WOS记录号: WOS:000170838400019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65437
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: Beijing Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China

Recommended Citation:
Ma, WB,Feng, BF,Tao, QM. Murine antibody against E2 can capture hepatitis C virus in vitro[J]. CHINESE MEDICAL JOURNAL,2001,114(8):860-863.
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