学科主题临床医学
Murine antibody against E2 can capture hepatitis C virus in vitro
Ma, WB; Feng, BF; Tao, QM
关键词hepatitis C virus neutralizing antibody envelop protein
刊名CHINESE MEDICAL JOURNAL
2001-08-01
114期:8页:860-863
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]HYPERVARIABLE REGION-1 ; HYPERIMMUNE SERUM ; INFECTION
英文摘要

Objective To find neutralizing antibody candidates against hepatitis C virus (HCV) infection.

Methods We constructed two eukaryotic expression vectors which contained the E1 and E2 gene of HCV, and detected their expression in mammalian cells with transient expression. BALB/c mice were given subculaneous injections of constructed vectors combined with the IL-2 gene intraepidermally and evaluated for induced humoral immune responses by enzyme-linked immunosorbent assay (ELISA). We used an antibody-virus interaction assay to analyze the interaction of the antisera and HCV viral particles in vitro.

Results Anti E1 and anti-E2 antisera were obtained from immunized mice. The serum of mice immunized with the E2 gene immunoprecipitated the HCV isolate in source serum and reacted with the isolates unrelated to the original one.

Conclusions Anti-E2 antibody in induced mice can cross-reactively capture HCV particles, highlighting the possibility of generating broadly reactive anti-E2 antibodies.

语种英语
WOS记录号WOS:000170838400019
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65437
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位Beijing Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
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Ma, WB,Feng, BF,Tao, QM. Murine antibody against E2 can capture hepatitis C virus in vitro[J]. CHINESE MEDICAL JOURNAL,2001,114(8):860-863.
APA Ma, WB,Feng, BF,&Tao, QM.(2001).Murine antibody against E2 can capture hepatitis C virus in vitro.CHINESE MEDICAL JOURNAL,114(8),860-863.
MLA Ma, WB,et al."Murine antibody against E2 can capture hepatitis C virus in vitro".CHINESE MEDICAL JOURNAL 114.8(2001):860-863.
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