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学科主题口腔医学
Secreted Phosphoprotein 24 kD (Spp24) and Spp14 Affect TGF-beta Induced Bone Formation Differently
Tian, Haijun1; Bi, Xiaoda2,7; Li, Chen-Shuang3; Zhao, Ke-Wei4; Brochmann, Elsa J.4,5,6; Montgomery, Scott R.8; Aghdasi, Bayan9; Chen, Deyu1; Daubs, Michael D.8; Wang, Jeffrey C.8; Murray, Samuel S.4,5,6
刊名PLOS ONE
2013-08-26
DOI10.1371/journal.pone.0072645
8期:8
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者Department of Veterans Affairs Biomedical Laboratory Research ; Development and Rehabilitation Research and Development Services ; Department of Veterans Affairs Biomedical Laboratory Research ; Development and Rehabilitation Research and Development Services
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]GROWTH-FACTOR-BETA ; MORPHOGENETIC PROTEIN-2 ; LIGAND-BINDING ; TRANSFORMING GROWTH-FACTOR-BETA-1 ; CRYSTAL-STRUCTURE ; RECEPTOR ; MATRIX ; ANTAGONISTS ; MUTAGENESIS ; EXPRESSION
英文摘要

Transforming growth factor-beta (TGF-beta) and bone morphogenetic proteins (BMPs) have opposing but complementary functions in directing bone growth, repair, and turnover. Both are found in the bone matrix. Proteins that bind to and affect the activity of these growth factors will determine the relative abundance of the growth factors and, therefore, regulate bone formation. Secreted phosphoprotein 24 kD (Spp24) is a bone matrix protein that has been demonstrated to bind to and affect the activity of BMPs. The arginine-rich carboxy terminus of Spp24 is proteolytically processed to produce three other predictable truncation products (Spp18.1, Spp16.0, and Spp14.5). In this work, we report that kinetic data obtained by surface plasmon resonance demonstrate that Spp24 and the three C-terminal truncation products all bind to TGF-beta 1 and TGF-beta 2 with a similar but somewhat less affinity than they bind BMP-2; that, as in the case of BMP-2, the full-length (FL) form of Spp24 binds TGF-beta with greater affinity than do the truncation products; that FL-Spp24 inhibits TGF-beta 2 induced bone formation in vivo, but Spp14.5 does not; and that co-administration of FL-Spp24 or Spp14.5 with TGF-beta 2 in vivo is associated with a reduction in the amount of cartilage, relative to new bone, present at the site of injection. This finding is consistent with the observation that low-dose TGF-beta administration in vivo is associated with greater bone formation than high-dose TGF-beta administration, and suggests that one function of Spp24 and its truncation products is to down-regulate local TGF-beta activity or availability during bone growth and development. The similarities and differences of the interactions between Spp24 proteins and TGF-beta compared to the interaction of the Spp24 proteins and BMPs have significant implications with respect to the regulation of bone metabolism and with respect to engineering therapeutic proteins for skeletal disorders.

语种英语
所属项目编号1I01BX000511 ; 1I0RX000383
资助者Department of Veterans Affairs Biomedical Laboratory Research ; Development and Rehabilitation Research and Development Services ; Department of Veterans Affairs Biomedical Laboratory Research ; Development and Rehabilitation Research and Development Services
WOS记录号WOS:000324228800081
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65449
专题北京大学口腔医学院_牙周科
作者单位1.Second Mil Med Univ, Changzheng Hosp, Dept Orthopaed Surg, Shanghai, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100871, Peoples R China
3.VA Greater Los Angeles Healthcare Syst, Res Serv, Educ & Clin Ctr, North Hills, CA USA
4.VA Greater Los Angeles Healthcare Syst, Educ & Clin Ctr, Geriatr Res, North Hills, CA USA
5.Univ Calif Los Angeles, Dept Med, Los Angeles, CA 90024 USA
6.Univ Calif Los Angeles, Dept Physiol, Los Angeles, CA 90024 USA
7.Univ Calif Los Angeles, Dept Orthopaed Surg, Los Angeles, CA USA
8.309th Hosp PLA, Dept Ophthalmol, Beijing, Peoples R China
9.Methodist Hosp Syst, Dept Orthopaed Surg, Houston, TX USA
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GB/T 7714
Tian, Haijun,Bi, Xiaoda,Li, Chen-Shuang,et al. Secreted Phosphoprotein 24 kD (Spp24) and Spp14 Affect TGF-beta Induced Bone Formation Differently[J]. PLOS ONE,2013,8(8).
APA Tian, Haijun.,Bi, Xiaoda.,Li, Chen-Shuang.,Zhao, Ke-Wei.,Brochmann, Elsa J..,...&Murray, Samuel S..(2013).Secreted Phosphoprotein 24 kD (Spp24) and Spp14 Affect TGF-beta Induced Bone Formation Differently.PLOS ONE,8(8).
MLA Tian, Haijun,et al."Secreted Phosphoprotein 24 kD (Spp24) and Spp14 Affect TGF-beta Induced Bone Formation Differently".PLOS ONE 8.8(2013).
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