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学科主题: 临床医学
题名:
In vivo evaluation of bone marrow stromal-derived osteoblasts-porous calcium phosphate ceramic composites as bone graft substitute for lumbar intervertebral spinal fusion
作者: Kai, T; Shao-qing, G; Geng-ting, D
关键词: bone marrow stromal cell ; osteoblasts ; calcium phosphate ceramics ; recombinant human bone morphogenetic protein-2 ; interbody fusion ; lumbar spine ; bone graft substitutes
刊名: SPINE
发表日期: 2003-08-01
卷: 28, 期:15, 页:1653-1658
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Clinical Neurology ; Orthopedics
研究领域[WOS]: Neurosciences & Neurology ; Orthopedics
关键词[WOS]: MESENCHYMAL STEM-CELLS ; CORALLINE HYDROXYAPATITE ; MORPHOGENETIC PROTEIN-2 ; MODEL ; DIFFERENTIATION ; DEFECTS ; CARRIER ; GROWTH ; MATRIX ; VITRO
英文摘要:

Study Design. Autogenous bone marrow stromal-derived osteoblasts-porous calcium phosphate ceramic composites were constructed in vitro under cell culture for 48 hours and implanted as a bone graft substitute for lumbar intervertebral spinal fusion in rabbits.

Objectives. To evaluate the efficacy of autogenous bone marrow stromal-derived osteoblasts-porous calcium phosphate ceramic composites as an alternative to autogenous graft materials in a lumbar interbody spinal fusion model.

Summary of Background Data. Bone marrow contains a population of rare progenitor cells capable of differentiating into bone, cartilage, muscle, tendon, and other connective tissues. These cells can be induced and differentiated into osteogenic osteoblasts with addition of osteogenic supplements. Combining bone marrow stromal-derived osteoblasts with porous ceramics gave rise to bone tissue in subcutaneous sites and repaired critical size segmental femoral defects. Little work has been done in the spine to assess fusion rates and associated biomechanical characteristics.

Methods. Five experimental groups were evaluated: sham operation ( Group I); porous calcium phosphate ceramics alone ( Group II); autogenous tricortical iliac crest ( Group III); bone marrow stromal-derived osteoblasts-calcium phosphate ceramic composites ( Group IV); bone marrow stromal-derived osteoblasts-calcium phosphate ceramic composites with rhBMP-2 ( Group V). All rabbits were killed 12 weeks after surgery, and the spinal fusion segments underwent the evaluation of gross inspection, manual palpation, radiography, computed tomography, nondestructive biomechanical testing, and histologic analysis.

Results. Successful spinal fusion was achieved by manual palpation in 100% (6/6) of animals in Group IV and Group V, 66.7% (4/6) in Group III, 50% (3/6) in Group II, and 0% (0/6) in Group I. Radiographic studies showed that minimal disc height loss was observed with ceramic blocks than with autograft. Biomechanical testingconfirmed that spines from Group IV and Group V were statistically significantly stiffer in flexion, extension, left and right bending, and left and right torsion than Group III and Group II. Histologic analysis demonstrated a qualitative increase of bone formation in fusion mass in Group IV and Group V versus all other groups. The size of fusion mass and the stiffness of fusion segments were greatest in Group V.

Conclusion. The results indicate that bone marrow stromal-derived osteoblasts-calcium phosphate ceramic composites may provide an alternative to autogenous graft materials for lumbar interbody spinal fusion. Adding recombinant human bone morphogenetic protein-2 into the composites may reinforce the biomechanical stiffness for spinal fusion segments. Porous calcium phosphate ceramics alone were not suitable as a bone graft substitute for lumbar interbody spinal fusion.

语种: 英语
WOS记录号: WOS:000184511900005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65454
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100083, Peoples R China

Recommended Citation:
Kai, T,Shao-qing, G,Geng-ting, D. In vivo evaluation of bone marrow stromal-derived osteoblasts-porous calcium phosphate ceramic composites as bone graft substitute for lumbar intervertebral spinal fusion[J]. SPINE,2003,28(15):1653-1658.
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