北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 骨肿瘤科  > 期刊论文
学科主题: 临床医学
题名:
Downregulation of SS18-SSX1 expression by small interfering RNA inhibits growth and induces apoptosis in human synovial sarcoma cell line HS-SY-II in vitro
作者: Peng, Changliang; Guo, Wei; Yang, Yi; Zhao, Hui
关键词: small interfering RNA ; SS18-SSX1 ; synovial sarcomas
刊名: EUROPEAN JOURNAL OF CANCER PREVENTION
发表日期: 2008-10-01
DOI: 10.1097/CEJ.0b013e328305a11b
卷: 17, 期:5, 页:392-398
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: GENE-EXPRESSION ; MAMMALIAN-CELLS ; FUSION GENE ; SSX ; IDENTIFICATION ; TRANSLOCATION ; MECHANISMS ; SUPPRESS ; THERAPY ; SIRNAS
英文摘要:

Substantial evidence indicates that the characteristic SS18-SSX fusion gene may play an important role in synovial sarcoma development and progression. For obtaining better insights into the genetic alterations and molecular mechanisms involved in synovial sarcomas and for developing novel therapeutic strategies for this disease, we first examined the efficiency of small interfering RNAs (siRNAs) targeting the SS18-SSX1 fusion gene in knocking down its expression in the human synovial sarcoma cell line HS-SY-II, and then evaluated the effects of downregulation of this gene on apoptosis, apoptosis-related gene expression, growth regulatory proteins, and the growth of tumor cells in vitro. We observed a marked decrease (by more than 87.6%) in SS18-SSX1 expression levels in cells transfected with a plasmid expressing hairpin siRNA for this gene, which was accompanied by (i) reduction in protein levels of cyclin D1 and cyclin A, (ii) reduction in antiapoptotic protein Bcl-2 and activation of caspase 3/apoptosis, and (iii) growth inhibition of HS-SY-II cells in vitro. Our results demonstrate that siRNA targeting of SS18-SSX1 may have therapeutic potential in the treatment of synovial sarcomas.

语种: 英语
所属项目编号: A899
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000259013000003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65456
Appears in Collections:北京大学第二临床医学院_骨肿瘤科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Peoples Hosp, Musculoskeletal Tumor Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Peng, Changliang,Guo, Wei,Yang, Yi,et al. Downregulation of SS18-SSX1 expression by small interfering RNA inhibits growth and induces apoptosis in human synovial sarcoma cell line HS-SY-II in vitro[J]. EUROPEAN JOURNAL OF CANCER PREVENTION,2008,17(5):392-398.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Peng, Changliang]'s Articles
[Guo, Wei]'s Articles
[Yang, Yi]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Peng, Changliang]‘s Articles
[Guo, Wei]‘s Articles
[Yang, Yi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace