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学科主题临床医学
Downregulation of SS18-SSX1 expression by small interfering RNA inhibits growth and induces apoptosis in human synovial sarcoma cell line HS-SY-II in vitro
Peng, Changliang; Guo, Wei; Yang, Yi; Zhao, Hui
关键词small interfering RNA SS18-SSX1 synovial sarcomas
刊名EUROPEAN JOURNAL OF CANCER PREVENTION
2008-10-01
DOI10.1097/CEJ.0b013e328305a11b
17期:5页:392-398
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]GENE-EXPRESSION ; MAMMALIAN-CELLS ; FUSION GENE ; SSX ; IDENTIFICATION ; TRANSLOCATION ; MECHANISMS ; SUPPRESS ; THERAPY ; SIRNAS
英文摘要

Substantial evidence indicates that the characteristic SS18-SSX fusion gene may play an important role in synovial sarcoma development and progression. For obtaining better insights into the genetic alterations and molecular mechanisms involved in synovial sarcomas and for developing novel therapeutic strategies for this disease, we first examined the efficiency of small interfering RNAs (siRNAs) targeting the SS18-SSX1 fusion gene in knocking down its expression in the human synovial sarcoma cell line HS-SY-II, and then evaluated the effects of downregulation of this gene on apoptosis, apoptosis-related gene expression, growth regulatory proteins, and the growth of tumor cells in vitro. We observed a marked decrease (by more than 87.6%) in SS18-SSX1 expression levels in cells transfected with a plasmid expressing hairpin siRNA for this gene, which was accompanied by (i) reduction in protein levels of cyclin D1 and cyclin A, (ii) reduction in antiapoptotic protein Bcl-2 and activation of caspase 3/apoptosis, and (iii) growth inhibition of HS-SY-II cells in vitro. Our results demonstrate that siRNA targeting of SS18-SSX1 may have therapeutic potential in the treatment of synovial sarcomas.

语种英语
WOS记录号WOS:000259013000003
项目编号A899
资助机构National Natural Science Foundation of China
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65456
专题北京大学第二临床医学院_骨肿瘤科
北京大学药学院_天然药物与仿生药物国家重点实验室
作者单位Peking Univ, Peoples Hosp, Musculoskeletal Tumor Ctr, Beijing 100044, Peoples R China
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GB/T 7714
Peng, Changliang,Guo, Wei,Yang, Yi,et al. Downregulation of SS18-SSX1 expression by small interfering RNA inhibits growth and induces apoptosis in human synovial sarcoma cell line HS-SY-II in vitro[J]. EUROPEAN JOURNAL OF CANCER PREVENTION,2008,17(5):392-398.
APA Peng, Changliang,Guo, Wei,Yang, Yi,&Zhao, Hui.(2008).Downregulation of SS18-SSX1 expression by small interfering RNA inhibits growth and induces apoptosis in human synovial sarcoma cell line HS-SY-II in vitro.EUROPEAN JOURNAL OF CANCER PREVENTION,17(5),392-398.
MLA Peng, Changliang,et al."Downregulation of SS18-SSX1 expression by small interfering RNA inhibits growth and induces apoptosis in human synovial sarcoma cell line HS-SY-II in vitro".EUROPEAN JOURNAL OF CANCER PREVENTION 17.5(2008):392-398.
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