北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Application of high-performance liquid chromatography-mass spectrometry to detection of diuretics in human urine
作者: Qin, Y; Wang, XB; Wang, C; Zhao, M; Wu, MT; Xu, YX; Peng, SQ
关键词: diuretics
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2003-08-25
DOI: 10.1016/S1570-0232(03)00422-7
卷: 794, 期:1, 页:193-203
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: DOPING CONTROL ; AGENTS ; HPLC
英文摘要:

A rapid. sensitive and reliable high-performance liquid chromatographic-mass spectrometric method for the detection of 25 diuretics in human urine has been developed. Atmosphere pressure chemical ionization (APCI) and electrospray ionization (ESI) modes were evaluated. A 2-ml volume of urine was extracted under basic conditions and separated on an Agilent Zorbax SB-C-18 column (150 x 2.1 mm, 5 mum). The mobile phase consisted of formic ammonium-formic acid buffer (pH 3.5) and acetonitrile. The effects of capillary temperature, sheath gas pressure and compositions of mobile phase on the sensitivity were studied. The recoveries of most of the diuretics were 75-95%. In the full scan mode, the limits of detection of the 25 diuretics were 0.25-25 ng/ml for APCI and 0.6-250 ng/ml for ESI. Under the optimal conditions, 14 diuretics from authentic urine samples were detected successfully by LC-APCI-MS. To obtain more fragmentation information on the chemical structure for positive confirmation, tandem mass analysis was also investigated. (C) 2003 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000184526000021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65458
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Coll Pharmaceut Sci, Beijing 100083, Peoples R China
2.Natl Res Inst Sports Med, China Doping Control Ctr, Beijing 100029, Peoples R China

Recommended Citation:
Qin, Y,Wang, XB,Wang, C,et al. Application of high-performance liquid chromatography-mass spectrometry to detection of diuretics in human urine[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2003,794(1):193-203.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Qin, Y]'s Articles
[Wang, XB]'s Articles
[Wang, C]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Qin, Y]‘s Articles
[Wang, XB]‘s Articles
[Wang, C]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace