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Application of high-performance liquid chromatography-mass spectrometry to detection of diuretics in human urine
Qin, Y; Wang, XB; Wang, C; Zhao, M; Wu, MT; Xu, YX; Peng, SQ
关键词diuretics
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2003-08-25
DOI10.1016/S1570-0232(03)00422-7
794期:1页:193-203
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]DOPING CONTROL ; AGENTS ; HPLC
英文摘要

A rapid. sensitive and reliable high-performance liquid chromatographic-mass spectrometric method for the detection of 25 diuretics in human urine has been developed. Atmosphere pressure chemical ionization (APCI) and electrospray ionization (ESI) modes were evaluated. A 2-ml volume of urine was extracted under basic conditions and separated on an Agilent Zorbax SB-C-18 column (150 x 2.1 mm, 5 mum). The mobile phase consisted of formic ammonium-formic acid buffer (pH 3.5) and acetonitrile. The effects of capillary temperature, sheath gas pressure and compositions of mobile phase on the sensitivity were studied. The recoveries of most of the diuretics were 75-95%. In the full scan mode, the limits of detection of the 25 diuretics were 0.25-25 ng/ml for APCI and 0.6-250 ng/ml for ESI. Under the optimal conditions, 14 diuretics from authentic urine samples were detected successfully by LC-APCI-MS. To obtain more fragmentation information on the chemical structure for positive confirmation, tandem mass analysis was also investigated. (C) 2003 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000184526000021
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65458
专题北京大学药学院
作者单位1.Peking Univ, Coll Pharmaceut Sci, Beijing 100083, Peoples R China
2.Natl Res Inst Sports Med, China Doping Control Ctr, Beijing 100029, Peoples R China
推荐引用方式
GB/T 7714
Qin, Y,Wang, XB,Wang, C,et al. Application of high-performance liquid chromatography-mass spectrometry to detection of diuretics in human urine[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2003,794(1):193-203.
APA Qin, Y.,Wang, XB.,Wang, C.,Zhao, M.,Wu, MT.,...&Peng, SQ.(2003).Application of high-performance liquid chromatography-mass spectrometry to detection of diuretics in human urine.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,794(1),193-203.
MLA Qin, Y,et al."Application of high-performance liquid chromatography-mass spectrometry to detection of diuretics in human urine".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 794.1(2003):193-203.
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