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学科主题基础医学
Hyperthermia induces epileptiform discharges in cultured rat cortical neurons
Wang, Ying-Ying3; Qin, Jiong3; Han, Ying3; Cai, Jie1,2; Xing, Guo-Gang1,2,4,5
关键词Febrile seizure Epileptiform discharge Hyperthermia GABA(B) receptor Cortical neuron In vitro
刊名BRAIN RESEARCH
2011-10-12
DOI10.1016/j.brainres.2011.08.027
1417期:15页:87-102
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]STROKE-INDUCED EPILEPSY ; IN-VITRO MODEL ; GABAERGIC SYNAPTIC-TRANSMISSION ; EXPERIMENTAL FEBRILE SEIZURES ; TEMPORAL-LOBE EPILEPSY ; METHYL-D-ASPARTATE ; ACID-B-RECEPTOR ; HIPPOCAMPAL-NEURONS ; IMMATURE RATS ; INDUCED EPILEPTOGENESIS
英文摘要

Febrile seizures (FS), or fever-induced seizures, are the most common form of seizures during childhood. Although simple FS are usually considered benign, prolonged or recurrent FS are proposed to increase the risk for developing subsequent temporal lobe epilepsy (TLE) in adults. The pathophysiology of FS is still largely unknown. In this study, by using whole-cell patch-clamp recording techniques, we demonstrated that hyperthermia (39-40 degrees C) induced a "febrile seizure-like event" expressed as spontaneous, recurrent, epileptiform discharges (SREDs) followed by a series of sustained depolarizations (SDs) in cultured rat cortical neurons (7-14 DIV). The SREDs were characterized by abruptly developing, paroxysmal depolarizing shifts (PDS) of membrane potential with high-frequency spike firing characteristic of electrographic seizures. Furthermore, we also found that hyperthermia induced persistent neuronal hyperexcitability as assessed by their intrinsic electrogenic characteristics which include: 1) depolarized resting potential (RP); 2) decreased input resistance (R(in).); 3) a marked decrease in amplitude, duration and afterhyperpolarization (AHP) of spontaneous action potentials; 4) a prominent reduction in action potential (AP) current threshold (I(th)) and potential threshold (TP); and 5) a dramatic shortened duration, decreased inter-spike intervals (IS!), and increased firing frequency of evoked action potentials. Additionally, our present study also revealed that baclofen (100 mu M), a specific GABA(B) receptor agonist, significantly repressed the hyperthermia-induced neuronal hyperexcitability and epileptiform discharges in cultured cortical neurons. The results suggest that hyperthermia may induce epileptiform activities in cultured cortical neurons by suppression of the GABA(B) receptor-mediated inhibition, in turn leading to the development of persistent neuronal hyperexcitability when the cells suffered from heating insult. This study provides a novel cellular model for studying the pathogenetic mechanisms of febrile seizures in vitro. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000296309700010
项目编号81072951 ; 61027001 ; 2008310005 ; 20117112079 ; 2008150007 ; 20090001110075
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Chinese Ministry of Education
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65471
专题北京大学基础医学院_神经生物学系
北京大学基础医学院
北京大学第二临床医学院_儿科
北京大学口腔医学院_综合治疗科
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100191, Peoples R China
3.Minist Educ, Key Lab Neurosci, Beijing 100191, Peoples R China
4.Minist Publ Hlth, Beijing 100191, Peoples R China
5.Peking Univ, Dept Pediat, Hosp 1, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Wang, Ying-Ying,Qin, Jiong,Han, Ying,et al. Hyperthermia induces epileptiform discharges in cultured rat cortical neurons[J]. BRAIN RESEARCH,2011,1417(15):87-102.
APA Wang, Ying-Ying,Qin, Jiong,Han, Ying,Cai, Jie,&Xing, Guo-Gang.(2011).Hyperthermia induces epileptiform discharges in cultured rat cortical neurons.BRAIN RESEARCH,1417(15),87-102.
MLA Wang, Ying-Ying,et al."Hyperthermia induces epileptiform discharges in cultured rat cortical neurons".BRAIN RESEARCH 1417.15(2011):87-102.
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