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学科主题: 基础医学
题名:
Hyperthermia induces epileptiform discharges in cultured rat cortical neurons
作者: Wang, Ying-Ying3; Qin, Jiong3; Han, Ying3; Cai, Jie1,2; Xing, Guo-Gang1,2,4,5
关键词: Febrile seizure ; Epileptiform discharge ; Hyperthermia ; GABA(B) receptor ; Cortical neuron ; In vitro
刊名: BRAIN RESEARCH
发表日期: 2011-10-12
DOI: 10.1016/j.brainres.2011.08.027
卷: 1417, 期:15, 页:87-102
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: STROKE-INDUCED EPILEPSY ; IN-VITRO MODEL ; GABAERGIC SYNAPTIC-TRANSMISSION ; EXPERIMENTAL FEBRILE SEIZURES ; TEMPORAL-LOBE EPILEPSY ; METHYL-D-ASPARTATE ; ACID-B-RECEPTOR ; HIPPOCAMPAL-NEURONS ; IMMATURE RATS ; INDUCED EPILEPTOGENESIS
英文摘要:

Febrile seizures (FS), or fever-induced seizures, are the most common form of seizures during childhood. Although simple FS are usually considered benign, prolonged or recurrent FS are proposed to increase the risk for developing subsequent temporal lobe epilepsy (TLE) in adults. The pathophysiology of FS is still largely unknown. In this study, by using whole-cell patch-clamp recording techniques, we demonstrated that hyperthermia (39-40 degrees C) induced a "febrile seizure-like event" expressed as spontaneous, recurrent, epileptiform discharges (SREDs) followed by a series of sustained depolarizations (SDs) in cultured rat cortical neurons (7-14 DIV). The SREDs were characterized by abruptly developing, paroxysmal depolarizing shifts (PDS) of membrane potential with high-frequency spike firing characteristic of electrographic seizures. Furthermore, we also found that hyperthermia induced persistent neuronal hyperexcitability as assessed by their intrinsic electrogenic characteristics which include: 1) depolarized resting potential (RP); 2) decreased input resistance (R(in).); 3) a marked decrease in amplitude, duration and afterhyperpolarization (AHP) of spontaneous action potentials; 4) a prominent reduction in action potential (AP) current threshold (I(th)) and potential threshold (TP); and 5) a dramatic shortened duration, decreased inter-spike intervals (IS!), and increased firing frequency of evoked action potentials. Additionally, our present study also revealed that baclofen (100 mu M), a specific GABA(B) receptor agonist, significantly repressed the hyperthermia-induced neuronal hyperexcitability and epileptiform discharges in cultured cortical neurons. The results suggest that hyperthermia may induce epileptiform activities in cultured cortical neurons by suppression of the GABA(B) receptor-mediated inhibition, in turn leading to the development of persistent neuronal hyperexcitability when the cells suffered from heating insult. This study provides a novel cellular model for studying the pathogenetic mechanisms of febrile seizures in vitro. (C) 2011 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 81072951 ; 61027001 ; 2008310005 ; 20117112079 ; 2008150007 ; 20090001110075
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Chinese Ministry of Education
WOS记录号: WOS:000296309700010
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65471
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100191, Peoples R China
3.Minist Educ, Key Lab Neurosci, Beijing 100191, Peoples R China
4.Minist Publ Hlth, Beijing 100191, Peoples R China
5.Peking Univ, Dept Pediat, Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Wang, Ying-Ying,Qin, Jiong,Han, Ying,et al. Hyperthermia induces epileptiform discharges in cultured rat cortical neurons[J]. BRAIN RESEARCH,2011,1417(15):87-102.
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