|Histone deacetylase 6 activity is critical for the metastasis of Burkitt s lymphoma cells|
|Ding, Ning; Ping, Lingyan; Feng, Lixia; Zheng, Xiaohui; Song, Yuqin; Zhu, Jun|
|关键词||Burkitt s lymphoma histone deacetylase 6 Cell shape elongation Metastasis Microtubule dynamics|
|刊名||CANCER CELL INTERNATIONAL|
|WOS标题词||Science & Technology|
|关键词[WOS]||HDAC6 ; INHIBITORS ; MICROTUBULES ; ACETYLATION ; MOTILITY ; PROTEIN ; CANCER ; P53|
Background: Burkitt s lymphoma is an aggressive malignancy with high risk of metastasis to extranodal sites, such as bone marrow and central nervous system. The prognosis of metastatic Burkitt s lymphoma is poor. Here we sought to identify a role of histone deacetylase 6 (HDAC6) in the metastasis of Burkitt s lymphoma cells.
Methods: Burkitt s lymphoma cells were pharmacologically treated with niltubacin, tubacin or sodium butyrate (NaB) or transfected with siRNAs to knock down the expression of HDAC6. Cell migration and invasion ability were measured by transwell assay, and cell cycle progression was analyzed by flow cytometry. Cell adhesion and proliferation was determined by CellTiter-Glo luminescent cell viability assay kit. Cell morphological alteration and microtubule stability were analyzed by immunofluorescence staining. Effect of niltubacin, tubacin and NaB on acetylated tubulin and siRNA efficacy were measured by western blotting.
Results: Suppression of histone deacetylase 6 activity significantly compromised the migration and invasion of Burkitt s lymphoma cells, without affecting cell proliferation and cell cycle progression. Mechanistic study revealed that HDAC6 modulated chemokine induced cell shape elongation and cell adhesion probably through its action on microtubule dynamics.
Conclusions: We identified a critical role of HDAC6 in the metastasis of Burkitt s lymphoma cells, suggesting that pharmacological inhibition of HDAC6 could be a promising strategy for the management of metastatic Burkitt s lymphoma.
|项目编号||81201873 ; 81470368 ; 7132050 ; 2011CB504303|
|资助机构||NSFC ; Beijing Natural Science Foundation ; 973 program|
|作者单位||Peking Univ, Canc Hosp & Inst, Dept Lymphoma, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China|
|Ding, Ning,Ping, Lingyan,Feng, Lixia,et al. Histone deacetylase 6 activity is critical for the metastasis of Burkitt s lymphoma cells[J]. CANCER CELL INTERNATIONAL,2014,14.|
|APA||Ding, Ning,Ping, Lingyan,Feng, Lixia,Zheng, Xiaohui,Song, Yuqin,&Zhu, Jun.(2014).Histone deacetylase 6 activity is critical for the metastasis of Burkitt s lymphoma cells.CANCER CELL INTERNATIONAL,14.|
|MLA||Ding, Ning,et al."Histone deacetylase 6 activity is critical for the metastasis of Burkitt s lymphoma cells".CANCER CELL INTERNATIONAL 14(2014).|