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学科主题: 临床医学
题名:
Histone deacetylase 6 activity is critical for the metastasis of Burkitt s lymphoma cells
作者: Ding, Ning; Ping, Lingyan; Feng, Lixia; Zheng, Xiaohui; Song, Yuqin; Zhu, Jun
关键词: Burkitt s lymphoma ; histone deacetylase 6 ; Cell shape elongation ; Metastasis ; Microtubule dynamics
刊名: CANCER CELL INTERNATIONAL
发表日期: 2014-12-05
DOI: 10.1186/s12935-014-0139-z
卷: 14
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: HDAC6 ; INHIBITORS ; MICROTUBULES ; ACETYLATION ; MOTILITY ; PROTEIN ; CANCER ; P53
英文摘要:

Background: Burkitt s lymphoma is an aggressive malignancy with high risk of metastasis to extranodal sites, such as bone marrow and central nervous system. The prognosis of metastatic Burkitt s lymphoma is poor. Here we sought to identify a role of histone deacetylase 6 (HDAC6) in the metastasis of Burkitt s lymphoma cells.

Methods: Burkitt s lymphoma cells were pharmacologically treated with niltubacin, tubacin or sodium butyrate (NaB) or transfected with siRNAs to knock down the expression of HDAC6. Cell migration and invasion ability were measured by transwell assay, and cell cycle progression was analyzed by flow cytometry. Cell adhesion and proliferation was determined by CellTiter-Glo luminescent cell viability assay kit. Cell morphological alteration and microtubule stability were analyzed by immunofluorescence staining. Effect of niltubacin, tubacin and NaB on acetylated tubulin and siRNA efficacy were measured by western blotting.

Results: Suppression of histone deacetylase 6 activity significantly compromised the migration and invasion of Burkitt s lymphoma cells, without affecting cell proliferation and cell cycle progression. Mechanistic study revealed that HDAC6 modulated chemokine induced cell shape elongation and cell adhesion probably through its action on microtubule dynamics.

Conclusions: We identified a critical role of HDAC6 in the metastasis of Burkitt s lymphoma cells, suggesting that pharmacological inhibition of HDAC6 could be a promising strategy for the management of metastatic Burkitt s lymphoma.

语种: 英语
所属项目编号: 81201873 ; 81470368 ; 7132050 ; 2011CB504303
项目资助者: NSFC ; Beijing Natural Science Foundation ; 973 program
WOS记录号: WOS:000364238700001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65478
Appears in Collections:北京大学临床肿瘤学院_淋巴肿瘤内科_期刊论文

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作者单位: Peking Univ, Canc Hosp & Inst, Dept Lymphoma, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China

Recommended Citation:
Ding, Ning,Ping, Lingyan,Feng, Lixia,et al. Histone deacetylase 6 activity is critical for the metastasis of Burkitt s lymphoma cells[J]. CANCER CELL INTERNATIONAL,2014,14.
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