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学科主题临床医学
Adrenomedullin inhibits the endothelin production induced by urotensin II in rat vascular smooth muscle cells
Qi, YF; Bu, DF; Yang, J; Zhang, ZK; Shi, YR; Pang, YZ; Tang, CS
关键词adrenomedullin urotensin II endothelin mRNA peptide/interaction
刊名CHINESE SCIENCE BULLETIN
2002-09-01
47期:17页:1457-1461
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]ENDOGENOUS LIGAND ; ORPHAN RECEPTOR ; COMPETITIVE PCR ; GPR14
英文摘要

The aim of this study was to observe the effects of adrenomedullin (ADM) on endothelin (ET) production induced by urotensin II (U II) in rat vascular smooth muscle cells (VSMCs). Cultured VSMCs which were incubated with U II (10(-8) mol/L) and with various concentrations of ADM were used to measure the VSMCs H-3-TdR incorporation, the activity of extracellular signal-regulated kinase (ERK), the amount of ET mRNA and ET production in VSMCs. In this work we found that incubation with U II (10(-8) mol/L) increased obviously the amount of ET mRNA in VSMCs and ET production in medium, however, co-incubation with ADM (10(-10)-10(-8) mol/L) and U II (10(-8) mol/L) reduced the amount of ET mRNA by 15%, 24% and 45% (P< 0.01) respectively, compared with U I alone. The content of ET in medium was 14.13, 11.38 and 11.00 pg/mL. ADM alone (10(-8) mol/L) had no effect on ET production in VSMCs. U II (10(-8) mol/L) promoted the H-3-TdR incorporation and activity of ERK in VSMCs. ADM inhibited VSMCS H-3-TdR incorporation and activation of ERK in a concentration-dependent manner. Compared with U II group, after co-incubation with ADM (10(-10)-10(-8) mol/L) and U II (10(-8) mol/L) the VSMCS H-3-TdR incorporation was decreased by 7% (P > 0.05), 32% (P < 0.05) and 41% (P < 0.01), respectively, and the activity of ERK was decreased by 24% (P > 0.05), 32% (P < 0.05) and 36% (P < 0.05), respectively, in a concentration-dependent manner. The results show that in cultured VSMCs ADM inhibits ET mRNA expression, ET production and proliferation stimulated by U II, and that inhibitory effect of ADM on U II bioaction could be mediated through inhibiting MAPK pathway.

语种英语
WOS记录号WOS:000177653300011
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65504
专题北京大学第一临床医学院_心血管内科
作者单位1.Peking Univ, Inst Cardiovasc Dis, First Hosp, Beijing 100034, Peoples R China
2.Phoenix Pharmaceut Inc, Mt View, CA 94043 USA
3.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Qi, YF,Bu, DF,Yang, J,et al. Adrenomedullin inhibits the endothelin production induced by urotensin II in rat vascular smooth muscle cells[J]. CHINESE SCIENCE BULLETIN,2002,47(17):1457-1461.
APA Qi, YF.,Bu, DF.,Yang, J.,Zhang, ZK.,Shi, YR.,...&Tang, CS.(2002).Adrenomedullin inhibits the endothelin production induced by urotensin II in rat vascular smooth muscle cells.CHINESE SCIENCE BULLETIN,47(17),1457-1461.
MLA Qi, YF,et al."Adrenomedullin inhibits the endothelin production induced by urotensin II in rat vascular smooth muscle cells".CHINESE SCIENCE BULLETIN 47.17(2002):1457-1461.
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