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学科主题: 医学信息学
题名:
Molecular characterization of pncA gene mutations in Mycobacterium tuberculosis clinical isolates from China
作者: Hou, L; Osei-Hyiaman, D; Zhang, Z; Wang, B; Yang, A; Kano, K
刊名: EPIDEMIOLOGY AND INFECTION
发表日期: 2000-04-01
卷: 124, 期:2, 页:227-232
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Public, Environmental & Occupational Health ; Infectious Diseases
研究领域[WOS]: Public, Environmental & Occupational Health ; Infectious Diseases
关键词[WOS]: PYRAZINAMIDE RESISTANCE ; IDENTIFICATION
英文摘要:

A sample of 35 pyrazinamide (PZA)-resistant and 30 PZA-susceptible clinical isolates recovered from Beijing and Taiyuan City, China were characterized by SSCP and sequence analysis for mutations in the pncA gene that encodes the Mycobacterium tuberculosis PZase. The purpose of this study was to understand the molecular basis and the characteristics of pncA gene mutations and its relation to PZA resistance in M. tuberculosis strains from China. Several mutations with base changes leading to amino acid substitutions were found in the PZA-resistant isolates. No mutations were seen in the 243 PZA-susceptible isolates. Among the 35 PZA-resistant isolates, 32 isolates (91.4 %) had nucleotide substitutions, insertions and deletions that resulted in amino-acid substitution; or frameshifts in some strains. Other previously uncharacterized mutations were found as follows: Asn118-->Thr, CG insertion at position 501; CC insertion at nucleotide position 403; a 8 base-pair deletion at start codon; Pro54-->Thr; AG insertion at 368; Tyr41-->His, Ser88-->stop, and A insertion at nucleotide position 301. IS6110 subtyping revealed that each strain was unique; indicative of the epidemiologic independence of the isolates.

语种: 英语
WOS记录号: WOS:000086886700007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65511
Appears in Collections:北京大学医学信息学中心_期刊论文

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作者单位: 1.Univ Tsukuba, Dept Med Microbiol & Infect Dis, Tsukuba, Ibaraki 3058575, Japan
2.Univ Tsukuba, Inst Community Med, Tsukuba, Ibaraki 3058575, Japan
3.Beijing Med Univ, Med Informat Ctr, Beijing 100083, Peoples R China
4.Univ Tsukuba, Grad Sch Med, Tsukuba, Ibaraki 3058575, Japan

Recommended Citation:
Hou, L,Osei-Hyiaman, D,Zhang, Z,et al. Molecular characterization of pncA gene mutations in Mycobacterium tuberculosis clinical isolates from China[J]. EPIDEMIOLOGY AND INFECTION,2000,124(2):227-232.
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