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学科主题: 基础医学
题名:
MEK1-ERKs signal cascade is required for the replication of Enterovirus 71 (EV71)
作者: Wang, Bo1; Zhang, Hao1; Zhu, Meng1; Luo, Zhijun2; Peng, Yihong1
关键词: EV71 ; Viral replication cycle ; MEK1 ; MEK2 ; ERK ; siRNAs
刊名: ANTIVIRAL RESEARCH
发表日期: 2012
DOI: 10.1016/j.antivira1.2011.11.001
卷: 93, 期:1, 页:110-117
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Virology
研究领域[WOS]: Pharmacology & Pharmacy ; Virology
关键词[WOS]: KINASE PATHWAYS ; VIRUS GROWTH ; PROTEIN ; ACTIVATION ; INFECTION ; ICP10 ; MEK2 ; PHOSPHORYLATION ; PROLIFERATION ; COMPARTMENTS
英文摘要:

The role of the MEK1-ERK signaling cascade in the replication cycle of Enterovirus 71 (EV71), the primary cause of hand, foot and mouth disease (HFMD), has been analyzed. In vitro infection with EV71 induced a biphasic activation of ERK. The two phases of activation appeared to be triggered by different mechanisms, with the first phase being activated by the binding of viral particles to the membrane receptor of host cells and the second probably being in response to the production of new virus particles. Inhibition of ERK activation by U0126 was found to severely impair virus production. A similar reduction in EV71 replication was also observed when MEK1 expression was subject to knockdown using specific siRNAs. By contrast knockdown of MEK2 expression showed that it was dispensable for virus replication cycle, despite both MEK isoforms being activated and translocated to the nucleus equally well in response to virus infection. Overall, this study suggests distinct functions of the two isoforms of MEK in EV71 replication cycle, with an essential role for MEK1 in stimulating the ERK signaling cascade to promote virus replication. Taken together with our previous work on herpes simplex virus type 2 (HSV2) this study highlights MEK1 as a potential broad antiviral molecular target. (C) 2011 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2007AA02Z317 ; 30470084
项目资助者: National High-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China
WOS记录号: WOS:000300802100014
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65517
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Peking Univ, Dept Microbiol, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA

Recommended Citation:
Wang, Bo,Zhang, Hao,Zhu, Meng,et al. MEK1-ERKs signal cascade is required for the replication of Enterovirus 71 (EV71)[J]. ANTIVIRAL RESEARCH,2012,93(1):110-117.
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