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学科主题基础医学
MEK1-ERKs signal cascade is required for the replication of Enterovirus 71 (EV71)
Wang, Bo1; Zhang, Hao1; Zhu, Meng1; Luo, Zhijun2; Peng, Yihong1
关键词EV71 Viral replication cycle MEK1 MEK2 ERK siRNAs
刊名ANTIVIRAL RESEARCH
2012
DOI10.1016/j.antivira1.2011.11.001
93期:1页:110-117
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy ; Virology
资助者National High-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National High-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China
研究领域[WOS]Pharmacology & Pharmacy ; Virology
关键词[WOS]KINASE PATHWAYS ; VIRUS GROWTH ; PROTEIN ; ACTIVATION ; INFECTION ; ICP10 ; MEK2 ; PHOSPHORYLATION ; PROLIFERATION ; COMPARTMENTS
英文摘要

The role of the MEK1-ERK signaling cascade in the replication cycle of Enterovirus 71 (EV71), the primary cause of hand, foot and mouth disease (HFMD), has been analyzed. In vitro infection with EV71 induced a biphasic activation of ERK. The two phases of activation appeared to be triggered by different mechanisms, with the first phase being activated by the binding of viral particles to the membrane receptor of host cells and the second probably being in response to the production of new virus particles. Inhibition of ERK activation by U0126 was found to severely impair virus production. A similar reduction in EV71 replication was also observed when MEK1 expression was subject to knockdown using specific siRNAs. By contrast knockdown of MEK2 expression showed that it was dispensable for virus replication cycle, despite both MEK isoforms being activated and translocated to the nucleus equally well in response to virus infection. Overall, this study suggests distinct functions of the two isoforms of MEK in EV71 replication cycle, with an essential role for MEK1 in stimulating the ERK signaling cascade to promote virus replication. Taken together with our previous work on herpes simplex virus type 2 (HSV2) this study highlights MEK1 as a potential broad antiviral molecular target. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
所属项目编号2007AA02Z317 ; 30470084
资助者National High-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; National High-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China
WOS记录号WOS:000300802100014
引用统计
被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65517
专题基础医学院_病原生物学系
作者单位1.Peking Univ, Dept Microbiol, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA
推荐引用方式
GB/T 7714
Wang, Bo,Zhang, Hao,Zhu, Meng,et al. MEK1-ERKs signal cascade is required for the replication of Enterovirus 71 (EV71)[J]. ANTIVIRAL RESEARCH,2012,93(1):110-117.
APA Wang, Bo,Zhang, Hao,Zhu, Meng,Luo, Zhijun,&Peng, Yihong.(2012).MEK1-ERKs signal cascade is required for the replication of Enterovirus 71 (EV71).ANTIVIRAL RESEARCH,93(1),110-117.
MLA Wang, Bo,et al."MEK1-ERKs signal cascade is required for the replication of Enterovirus 71 (EV71)".ANTIVIRAL RESEARCH 93.1(2012):110-117.
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