IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
A Systematic Analysis on DNA Methylation and the Expression of Both mRNA and microRNA in Bladder Cancer
Zhu, Jialou1,2; Jiang, Zhimao3; Gao, Fei1; Hu, Xueda1,4,5; Zhou, Liang3; Chen, Jiahao1; Luo, Huijuan1; Sun, Jihua1; Wu, Song3; Han, Yonghua3; Yin, Guangliang1; Chen, Maoshan1; Han, Zujing1; Li, Xianxin3; Huang, Yi3; Zhang, Weixing7; Zhou, Fangjian8; Chen, Tong9; Fa, Pingping3; Wang, Yong3; Sun, Liang3; Leng, Huimin3; Sun, Fenghao3,10; Liu, Yuchen3,9; Ye, Mingzhi1; Yang, Huanming1; Cai, Zhiming3,6; Gui, Yaoting3; Zhang, Xiuqing1
刊名PLOS ONE
2011-11-30
DOI10.1371/journal.pone.0028223
6期:11
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National High Technology Research and Development Program of China (863 Program) ; Promotion Program for Shenzhen Key Laboratory, Shenzhen, China ; National High Technology Research and Development Program of China (863 Program) ; Promotion Program for Shenzhen Key Laboratory, Shenzhen, China
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]SQUAMOUS-CELL CARCINOMA ; TUMOR-SUPPRESSOR GENE ; CERVICAL INTRAEPITHELIAL NEOPLASIA ; PROMOTER HYPERMETHYLATION ; BREAST-CANCER ; TARGET PREDICTIONS ; AXON GUIDANCE ; SLIT2 GENE ; GROWTH ; PROGRESSION
英文摘要

Background: DNA methylation aberration and microRNA (miRNA) deregulation have been observed in many types of cancers. A systematic study of methylome and transcriptome in bladder urothelial carcinoma has never been reported.

Methodology/Principal Findings: The DNA methylation was profiled by modified methylation-specific digital karyotyping (MMSDK) and the expression of mRNAs and miRNAs was analyzed by digital gene expression (DGE) sequencing in tumors and matched normal adjacent tissues obtained from 9 bladder urothelial carcinoma patients. We found that a set of significantly enriched pathways disrupted in bladder urothelial carcinoma primarily related to "neurogenesis" and "cell differentiation" by integrated analysis of -omics data. Furthermore, we identified an intriguing collection of cancer-related genes that were deregulated at the levels of DNA methylation and mRNA expression, and we validated several of these genes (HIC1, SLIT2, RASAL1, and KRT17) by Bisulfite Sequencing PCR and Reverse Transcription qPCR in a panel of 33 bladder cancer samples.

Conclusions/Significance: We characterized the profiles between methylome and transcriptome in bladder urothelial carcinoma, identified a set of significantly enriched key pathways, and screened four aberrantly methylated and expressed genes. Conclusively, our findings shed light on a new avenue for basic bladder cancer research.

语种英语
所属项目编号2009AA022707 ; CXB200903090055A ; CXB201005250016A
资助者National High Technology Research and Development Program of China (863 Program) ; Promotion Program for Shenzhen Key Laboratory, Shenzhen, China ; National High Technology Research and Development Program of China (863 Program) ; Promotion Program for Shenzhen Key Laboratory, Shenzhen, China
WOS记录号WOS:000298168100053
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65519
Collection北京大学深圳医院
作者单位1.Beijing Genom Inst Shenzhen, Shenzhen, Peoples R China
2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
3.Shenzhen Peoples Hosp, Dept Urol, Shenzhen, Peoples R China
4.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol,Shenzhen PKU HKUST Med Ctr, Shenzhen, Peoples R China
5.Chinese Acad Sci, Beijing Inst Genom, Beijing, Peoples R China
6.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
7.Second Peoples Hosp Shenzhen, Dept Urol, Shenzhen, Peoples R China
8.Zhengzhou Univ, Affiliated Hosp 1, Dept Urol, Zhengzhou, Peoples R China
9.Sun Yat Sen Univ, Ctr Canc, Dept Urol, Guangzhou 510275, Guangdong, Peoples R China
10.Shantou Univ, Coll Med, Shantou, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Jialou,Jiang, Zhimao,Gao, Fei,et al. A Systematic Analysis on DNA Methylation and the Expression of Both mRNA and microRNA in Bladder Cancer[J]. PLOS ONE,2011,6(11).
APA Zhu, Jialou.,Jiang, Zhimao.,Gao, Fei.,Hu, Xueda.,Zhou, Liang.,...&Zhang, Xiuqing.(2011).A Systematic Analysis on DNA Methylation and the Expression of Both mRNA and microRNA in Bladder Cancer.PLOS ONE,6(11).
MLA Zhu, Jialou,et al."A Systematic Analysis on DNA Methylation and the Expression of Both mRNA and microRNA in Bladder Cancer".PLOS ONE 6.11(2011).
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