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学科主题: 临床医学
题名:
High density lipoprotein suppresses lipoprotein associated phospholipase A2 in human monocytes-derived macrophages through peroxisome proliferator-activated receptor-gamma pathway
作者: Han Guan-ping1; Ren Jing-yi1; Qin Li2; Song Jun-xian1; Wang Lan1; Chen Hong1
关键词: atherosclerosis ; high-density lipoprotein ; lipoprotein-associated phospholipase A2 ; peroxisome proliferator-activated receptor-gamma
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2012-12-20
DOI: 10.3760/cma.j.issn.0366-6999.2012.24.028
卷: 125, 期:24, 页:4474-4480
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: CORONARY-HEART-DISEASE ; C-REACTIVE PROTEIN ; MIDDLE-AGED MEN ; FACTOR-ACETYLHYDROLASE ; GENE-EXPRESSION ; A(2) ; ATHEROSCLEROSIS ; RISK ; INFLAMMATION ; TARGET
英文摘要:

Background Lipoprotein-associated phospholipase A2 (Lp-PLA2) is mainly secreted by macrophages, serving as a specific marker of atherosclerotic plaque and exerting pro-atherogenic effects. It is known that high-density lipoprotein (HDL) plays an important role against atherosclerosis by inhibiting pro-inflammatory factors, however, the relationship between HDL and Lp-PLA2 remains elusive.

Methods In this study, reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and a platelet-activating factor (PAF) acetylhydrolase assay were performed to determine the Lp-PLA2 mRNA level, protein expression and activity in human monocyte-derived macrophages upon HDL treatment of different concentrations and durations. To investigate the underlying mechanism of HDL-induced Lp-PLA2 action, pioglitazone, a peroxisome proliferator-activated receptor-gamma (PPAR gamma) ligand, was introduced to human monocyte-derived macrophages and mRNA and protein levels of Lp-PLA2, as well as its activity, were determined.

Results Lp-PLA2 mRNA levels, protein expression and activity were significantly inhibited in response to HDL treatment in a dose and time dependent manner in human monocyte-derived macrophages. Pioglitazone treatment (1-10 ng/ml) upregulated the Lp-PLA2 mRNA level, protein expression and activity in human monocyte-derived macrophages, while the effects were markedly reversed by HDL. In addition, pioglitazone resulted in a significant increase in PPAR gamma phosphorylation in human monocyte-derived macrophages, which could be inhibited by HDL.

Conclusion These findings indicate that HDL suppresses the expression and activity of Lp-PLA2 in human monocyte-derived macrophages, and the underlying mechanisms may be mediated through the PPAR gamma pathway. Chin Med J 2012;125(24):4474-4480

语种: 英语
所属项目编号: 30570712 ; 30840040 ; 7092109
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000313933000029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65523
Appears in Collections:北京大学第二临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Cardiol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Lab Med, Beijing 100044, Peoples R China

Recommended Citation:
Han Guan-ping,Ren Jing-yi,Qin Li,et al. High density lipoprotein suppresses lipoprotein associated phospholipase A2 in human monocytes-derived macrophages through peroxisome proliferator-activated receptor-gamma pathway[J]. CHINESE MEDICAL JOURNAL,2012,125(24):4474-4480.
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