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High density lipoprotein suppresses lipoprotein associated phospholipase A2 in human monocytes-derived macrophages through peroxisome proliferator-activated receptor-gamma pathway
Han Guan-ping1; Ren Jing-yi1; Qin Li2; Song Jun-xian1; Wang Lan1; Chen Hong1
关键词atherosclerosis high-density lipoprotein lipoprotein-associated phospholipase A2 peroxisome proliferator-activated receptor-gamma
刊名CHINESE MEDICAL JOURNAL
2012-12-20
DOI10.3760/cma.j.issn.0366-6999.2012.24.028
125期:24页:4474-4480
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]CORONARY-HEART-DISEASE ; C-REACTIVE PROTEIN ; MIDDLE-AGED MEN ; FACTOR-ACETYLHYDROLASE ; GENE-EXPRESSION ; A(2) ; ATHEROSCLEROSIS ; RISK ; INFLAMMATION ; TARGET
英文摘要

Background Lipoprotein-associated phospholipase A2 (Lp-PLA2) is mainly secreted by macrophages, serving as a specific marker of atherosclerotic plaque and exerting pro-atherogenic effects. It is known that high-density lipoprotein (HDL) plays an important role against atherosclerosis by inhibiting pro-inflammatory factors, however, the relationship between HDL and Lp-PLA2 remains elusive.

Methods In this study, reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and a platelet-activating factor (PAF) acetylhydrolase assay were performed to determine the Lp-PLA2 mRNA level, protein expression and activity in human monocyte-derived macrophages upon HDL treatment of different concentrations and durations. To investigate the underlying mechanism of HDL-induced Lp-PLA2 action, pioglitazone, a peroxisome proliferator-activated receptor-gamma (PPAR gamma) ligand, was introduced to human monocyte-derived macrophages and mRNA and protein levels of Lp-PLA2, as well as its activity, were determined.

Results Lp-PLA2 mRNA levels, protein expression and activity were significantly inhibited in response to HDL treatment in a dose and time dependent manner in human monocyte-derived macrophages. Pioglitazone treatment (1-10 ng/ml) upregulated the Lp-PLA2 mRNA level, protein expression and activity in human monocyte-derived macrophages, while the effects were markedly reversed by HDL. In addition, pioglitazone resulted in a significant increase in PPAR gamma phosphorylation in human monocyte-derived macrophages, which could be inhibited by HDL.

Conclusion These findings indicate that HDL suppresses the expression and activity of Lp-PLA2 in human monocyte-derived macrophages, and the underlying mechanisms may be mediated through the PPAR gamma pathway. Chin Med J 2012;125(24):4474-4480

语种英语
WOS记录号WOS:000313933000029
项目编号30570712 ; 30840040 ; 7092109
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65523
专题北京大学第一临床医学院_药剂科
北京大学第二临床医学院_心血管内科
作者单位1.Peking Univ, Peoples Hosp, Dept Cardiol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Lab Med, Beijing 100044, Peoples R China
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GB/T 7714
Han Guan-ping,Ren Jing-yi,Qin Li,et al. High density lipoprotein suppresses lipoprotein associated phospholipase A2 in human monocytes-derived macrophages through peroxisome proliferator-activated receptor-gamma pathway[J]. CHINESE MEDICAL JOURNAL,2012,125(24):4474-4480.
APA Han Guan-ping,Ren Jing-yi,Qin Li,Song Jun-xian,Wang Lan,&Chen Hong.(2012).High density lipoprotein suppresses lipoprotein associated phospholipase A2 in human monocytes-derived macrophages through peroxisome proliferator-activated receptor-gamma pathway.CHINESE MEDICAL JOURNAL,125(24),4474-4480.
MLA Han Guan-ping,et al."High density lipoprotein suppresses lipoprotein associated phospholipase A2 in human monocytes-derived macrophages through peroxisome proliferator-activated receptor-gamma pathway".CHINESE MEDICAL JOURNAL 125.24(2012):4474-4480.
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