北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
High density lipoprotein suppresses lipoprotein associated phospholipase A2 in human monocytes-derived macrophages through peroxisome proliferator-activated receptor-gamma pathway
作者: Han Guan-ping1; Ren Jing-yi1; Qin Li2; Song Jun-xian1; Wang Lan1; Chen Hong1
关键词: atherosclerosis ; high-density lipoprotein ; lipoprotein-associated phospholipase A2 ; peroxisome proliferator-activated receptor-gamma
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2012-12-20
DOI: 10.3760/cma.j.issn.0366-6999.2012.24.028
卷: 125, 期:24, 页:4474-4480
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: CORONARY-HEART-DISEASE ; C-REACTIVE PROTEIN ; MIDDLE-AGED MEN ; FACTOR-ACETYLHYDROLASE ; GENE-EXPRESSION ; A(2) ; ATHEROSCLEROSIS ; RISK ; INFLAMMATION ; TARGET
英文摘要:

Background Lipoprotein-associated phospholipase A2 (Lp-PLA2) is mainly secreted by macrophages, serving as a specific marker of atherosclerotic plaque and exerting pro-atherogenic effects. It is known that high-density lipoprotein (HDL) plays an important role against atherosclerosis by inhibiting pro-inflammatory factors, however, the relationship between HDL and Lp-PLA2 remains elusive.

Methods In this study, reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and a platelet-activating factor (PAF) acetylhydrolase assay were performed to determine the Lp-PLA2 mRNA level, protein expression and activity in human monocyte-derived macrophages upon HDL treatment of different concentrations and durations. To investigate the underlying mechanism of HDL-induced Lp-PLA2 action, pioglitazone, a peroxisome proliferator-activated receptor-gamma (PPAR gamma) ligand, was introduced to human monocyte-derived macrophages and mRNA and protein levels of Lp-PLA2, as well as its activity, were determined.

Results Lp-PLA2 mRNA levels, protein expression and activity were significantly inhibited in response to HDL treatment in a dose and time dependent manner in human monocyte-derived macrophages. Pioglitazone treatment (1-10 ng/ml) upregulated the Lp-PLA2 mRNA level, protein expression and activity in human monocyte-derived macrophages, while the effects were markedly reversed by HDL. In addition, pioglitazone resulted in a significant increase in PPAR gamma phosphorylation in human monocyte-derived macrophages, which could be inhibited by HDL.

Conclusion These findings indicate that HDL suppresses the expression and activity of Lp-PLA2 in human monocyte-derived macrophages, and the underlying mechanisms may be mediated through the PPAR gamma pathway. Chin Med J 2012;125(24):4474-4480

语种: 英语
所属项目编号: 30570712 ; 30840040 ; 7092109
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000313933000029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65523
Appears in Collections:北京大学第二临床医学院_心血管内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Peoples Hosp, Dept Cardiol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Lab Med, Beijing 100044, Peoples R China

Recommended Citation:
Han Guan-ping,Ren Jing-yi,Qin Li,et al. High density lipoprotein suppresses lipoprotein associated phospholipase A2 in human monocytes-derived macrophages through peroxisome proliferator-activated receptor-gamma pathway[J]. CHINESE MEDICAL JOURNAL,2012,125(24):4474-4480.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Han Guan-ping]'s Articles
[Ren Jing-yi]'s Articles
[Qin Li]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Han Guan-ping]‘s Articles
[Ren Jing-yi]‘s Articles
[Qin Li]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace