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学科主题: 口腔医学
题名:
Porphyromonas gingivalis infection enhances Th17 responses for development of atherosclerosis
作者: Cai, Yu1,2; Kobayashi, Ryoki1; Hashizume-Takizawa, Tomomi1; Kurita-Ochiai, Tomoko1
关键词: Atherosclerosis ; Porphyromonas gingivalis ; Regulatory T cells ; T-helper 1 cells ; T-helper 17 cells
刊名: ARCHIVES OF ORAL BIOLOGY
发表日期: 2014-11-01
DOI: 10.1016/j.archoralbio.2014.07.012
卷: 59, 期:11, 页:1183-1191
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: REGULATORY T-CELLS ; DEFICIENT MICE ; TGF-BETA ; INFLAMMATION ; DISEASE ; MECHANISMS ; CYTOKINES ; PATHWAYS ; T(H)17 ; DIFFERENTIATION
英文摘要:

Objectives: Porphyromonas gingivalis has been shown to associate with the development of atherosclerosis. Recent studies indicate that IL-17-producing T helper 17 (Th17) cells have been correlated with the emergence of atherosclerosis. Therefore, we investigated whether the Th17 cell response and expression of Th17-related molecules, in contrast with Th1- and Treg cells, are enhanced by P. gingivalis-challenge in Apolipoprotein E knockout (ApoE KO) mice.

Design: Five mice were intravenously injected with P. gingivalis three times a week for 3 weeks and killed at 15 weeks of age. The proximal aorta lesion area, flow cytometry analysis and IL-17, IL-10, IFN-gamma, and IL-1 beta levels in splenic cultures, and expression of Th17-related molecules in spleen and hearts were examined.

Results: P. gingivalis-challenge showed notable accumulation of atherosclerotic plaques by Oil Red O-staining in ApoE KO mice. Intracellular cytokine staining revealed that significantly elevated CD4(+) interleukin (IL)-17A(+) T cells and slightly increased CD4(+)Foxp3(+) T cells was recognized in spleen cells of P. gingivalis-challenged mice compared with those from non-infected mice. P. gingivalis-challenge significantly increased IL-17 and IL-1 beta production and ROR gamma t expression in splenic cells. Furthermore, the expression of Th17-related genes such as IL-6, TGF-beta, ROR gamma t and STAT3 were elevated in splenic cells as well as heart tissue of P. gingivalis-challenged mice.

Conclusion: These results suggest that P. gingivalis infection may enhance pro-inflammatory Th17 cell responses in lesion areas and spleen, thereby accelerating atherosclerosis. (C) 2014 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 22390398 ; S1001024
项目资助者: Japan Society for the Promotion of Science ; Japan [MEXT for Private Universities of the Ministry of Education, Culture, Sports, Science and Technology, Japan
WOS记录号: WOS:000342244400008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65537
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Nihon Univ Sch Dent Matsudo, Dept Microbiol & Immunol, Matsudo, Chiba 2718587, Japan
2.Peking Univ Sch & Hosp Stomatol, Dept Periodontol, Beijing 100081, Peoples R China

Recommended Citation:
Cai, Yu,Kobayashi, Ryoki,Hashizume-Takizawa, Tomomi,et al. Porphyromonas gingivalis infection enhances Th17 responses for development of atherosclerosis[J]. ARCHIVES OF ORAL BIOLOGY,2014,59(11):1183-1191.
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