|Porphyromonas gingivalis infection enhances Th17 responses for development of atherosclerosis|
|Cai, Yu1,2; Kobayashi, Ryoki1; Hashizume-Takizawa, Tomomi1; Kurita-Ochiai, Tomoko1|
|关键词||Atherosclerosis Porphyromonas gingivalis Regulatory T cells T-helper 1 cells T-helper 17 cells|
|刊名||ARCHIVES OF ORAL BIOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Dentistry, Oral Surgery & Medicine|
|研究领域[WOS]||Dentistry, Oral Surgery & Medicine|
|关键词[WOS]||REGULATORY T-CELLS ; DEFICIENT MICE ; TGF-BETA ; INFLAMMATION ; DISEASE ; MECHANISMS ; CYTOKINES ; PATHWAYS ; T(H)17 ; DIFFERENTIATION|
Objectives: Porphyromonas gingivalis has been shown to associate with the development of atherosclerosis. Recent studies indicate that IL-17-producing T helper 17 (Th17) cells have been correlated with the emergence of atherosclerosis. Therefore, we investigated whether the Th17 cell response and expression of Th17-related molecules, in contrast with Th1- and Treg cells, are enhanced by P. gingivalis-challenge in Apolipoprotein E knockout (ApoE KO) mice.
Design: Five mice were intravenously injected with P. gingivalis three times a week for 3 weeks and killed at 15 weeks of age. The proximal aorta lesion area, flow cytometry analysis and IL-17, IL-10, IFN-gamma, and IL-1 beta levels in splenic cultures, and expression of Th17-related molecules in spleen and hearts were examined.
Results: P. gingivalis-challenge showed notable accumulation of atherosclerotic plaques by Oil Red O-staining in ApoE KO mice. Intracellular cytokine staining revealed that significantly elevated CD4(+) interleukin (IL)-17A(+) T cells and slightly increased CD4(+)Foxp3(+) T cells was recognized in spleen cells of P. gingivalis-challenged mice compared with those from non-infected mice. P. gingivalis-challenge significantly increased IL-17 and IL-1 beta production and ROR gamma t expression in splenic cells. Furthermore, the expression of Th17-related genes such as IL-6, TGF-beta, ROR gamma t and STAT3 were elevated in splenic cells as well as heart tissue of P. gingivalis-challenged mice.
Conclusion: These results suggest that P. gingivalis infection may enhance pro-inflammatory Th17 cell responses in lesion areas and spleen, thereby accelerating atherosclerosis. (C) 2014 Elsevier Ltd. All rights reserved.
|项目编号||22390398 ; S1001024|
|资助机构||Japan Society for the Promotion of Science ; Japan [MEXT for Private Universities of the Ministry of Education, Culture, Sports, Science and Technology, Japan|
|作者单位||1.Nihon Univ Sch Dent Matsudo, Dept Microbiol & Immunol, Matsudo, Chiba 2718587, Japan|
2.Peking Univ Sch & Hosp Stomatol, Dept Periodontol, Beijing 100081, Peoples R China
|Cai, Yu,Kobayashi, Ryoki,Hashizume-Takizawa, Tomomi,et al. Porphyromonas gingivalis infection enhances Th17 responses for development of atherosclerosis[J]. ARCHIVES OF ORAL BIOLOGY,2014,59(11):1183-1191.|
|APA||Cai, Yu,Kobayashi, Ryoki,Hashizume-Takizawa, Tomomi,&Kurita-Ochiai, Tomoko.(2014).Porphyromonas gingivalis infection enhances Th17 responses for development of atherosclerosis.ARCHIVES OF ORAL BIOLOGY,59(11),1183-1191.|
|MLA||Cai, Yu,et al."Porphyromonas gingivalis infection enhances Th17 responses for development of atherosclerosis".ARCHIVES OF ORAL BIOLOGY 59.11(2014):1183-1191.|