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iASPP is over-expressed in human non-small cell lung cancer and regulates the proliferation of lung cancer cells through a p53 associated pathway
Chen, Jinfeng1,2,3; Xie, Fei1,2; Zhang, Lijian1,2; Jiang, Wen G.3
刊名BMC CANCER
2010-12-30
DOI10.1186/1471-2407-10-694
10
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]ASPP PROTEINS ; PROMOTES APOPTOSIS ; LEUKEMIA ; FAMILY ; MUTATIONS ; BINDING ; MEMBERS ; LINES ; P73
英文摘要

Background: iASPP is a key inhibitor of tumour suppressor p53 and is found to be up-regulated in certain malignant conditions. The present study investigated the expression of iASPP in clinical lung cancer, a leading cancer type in the world, and the biological impact of this molecule on lung cancer cells.

Methods: iASPP protein levels in lung cancer tissues were evaluated using an immunohistochemical method. In vitro, iASPP gene expression was suppressed with a lentvirus-mediated shRNA method and the biological impact after knocking down iASSP on lung cancer cell lines was investigated in connection with the p53 expression status.

Results: We showed here that the expression of iASPP was significantly higher in lung cancer tissues compared with the adjacent normal tissues. iASPP shRNA treatment resulted in a down-regulation of iASPP in lung cancer cells. There was a subsequent reduction of cell proliferation of the two lung tumour cell lines A459 and 95D both of which had wild-type p53 expression. In contrast, reduction of iASPP in H1229 cells, a cell with little p53 expression, had no impact on its growth rate.

Conclusions: iASPP regulates the proliferation and motility of lung cancer cells. This effect is intimately associated with the p53 pathway. Together with the pattern of the over-expression in clinical lung cancers, it is concluded that iASPP plays an pivotal role in the progression of lung cancer and is a potential target for lung cancer therapy.

语种英语
WOS记录号WOS:000286315100001
资助机构UICC ; Cardiff University ; Albert Hung Foundation ; Cancer Research Wales
引用统计
被引频次:44[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65538
专题北京大学临床肿瘤学院_胸外科
北京大学第二临床医学院_乳腺外科
北京大学临床肿瘤学院_胸部肿瘤外二科
作者单位1.Peking Univ, Sch Oncol, Dept Thorac Surg, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
2.Beijing Canc Hosp & Inst, Beijing 100142, Peoples R China
3.Cardiff Univ, Sch Med, Metastasis & Angiogenesis Res Grp, Cardiff CF14 4XN, S Glam, Wales
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GB/T 7714
Chen, Jinfeng,Xie, Fei,Zhang, Lijian,et al. iASPP is over-expressed in human non-small cell lung cancer and regulates the proliferation of lung cancer cells through a p53 associated pathway[J]. BMC CANCER,2010,10.
APA Chen, Jinfeng,Xie, Fei,Zhang, Lijian,&Jiang, Wen G..(2010).iASPP is over-expressed in human non-small cell lung cancer and regulates the proliferation of lung cancer cells through a p53 associated pathway.BMC CANCER,10.
MLA Chen, Jinfeng,et al."iASPP is over-expressed in human non-small cell lung cancer and regulates the proliferation of lung cancer cells through a p53 associated pathway".BMC CANCER 10(2010).
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