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Three-dimensional quantitative analysis of chromosomes in the oocytes of aging mice during meiosis I in vitro
Tian, N.1; Zhang, L.2; Zheng, J. H.1; Lv, D. Y.2; Li, Y.2; Ma, W. Y.1
关键词Aging Oocytes Three-dimensional chromosomal morphology Meiosis I Two-photon laser scanning microscope
刊名THERIOGENOLOGY
2013-01-15
DOI10.1016/j.theriogenology.2012.08.010
79期:2页:249-256
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Reproductive Biology ; Veterinary Sciences
资助者National Nature Science Foundation of China ; Chinese National Basic Research Program ; Doctoral Program Research Fund of Chinese Ministry of Education ; Tsinghua University Initiative Scientific Research Program ; National Nature Science Foundation of China ; Chinese National Basic Research Program ; Doctoral Program Research Fund of Chinese Ministry of Education ; Tsinghua University Initiative Scientific Research Program
研究领域[WOS]Reproductive Biology ; Veterinary Sciences
关键词[WOS]SISTER-CHROMATID COHESION ; MOUSE OOCYTES ; HISTONE MODIFICATIONS ; GENE-EXPRESSION ; PORCINE OOCYTES ; ANEUPLOIDY ; AGE ; CONFIGURATIONS ; NONDISJUNCTION ; DEACETYLATION
英文摘要

The mechanism of senescence is very complicated and can involve formation of chromosome abnormalities and a decline in female fertility. In this study, 3-D visualization of fluorescently labeled chromosomes in oocytes from aging and pubertal mice during in vitro maturation was done with a two-photon laser scanning microscope. Differences between aging and pubertal groups at various maturation stages were analyzed quantitatively in terms of chromosomal morphology, shape, and spatial arrangement. Compared with the pubertal group, the chromosomal morphology of oocytes from aging mice changed: both the mean volume and the mean surface area of chromosomes increased by approximately 20% (P < 0.05) at prometaphase and metaphase of meiosis I (considered to be the weakly condensed folded form of the chromosomes). Furthermore, at these stages, the shape of the chromosomal array became rounder (roundness factor increased by approximately 10%; P < 0.001) and the adhesion among chromosomes became more severe (P < 0.001) at approximately the same stages. Additionally, trends over time for both chromosomal morphology and shape were quite distinct between oocytes from aging and pubertal mice. Interestingly, trends for mean distance were similar; therefore, aging did not seem to influence chromosome movement toward the metaphase plate. These morphologic results should be useful to study age-related degradation of oocyte quality and to interpret results derived from molecular biology. (C) 2013 Elsevier Inc. All rights reserved.

语种英语
所属项目编号90919012 ; 10874099 ; 2007CB5119004 ; 20090002110065 ; 2010THZ01
资助者National Nature Science Foundation of China ; Chinese National Basic Research Program ; Doctoral Program Research Fund of Chinese Ministry of Education ; Tsinghua University Initiative Scientific Research Program ; National Nature Science Foundation of China ; Chinese National Basic Research Program ; Doctoral Program Research Fund of Chinese Ministry of Education ; Tsinghua University Initiative Scientific Research Program
WOS记录号WOS:000313311300006
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65541
专题基础医学院
作者单位1.Tsinghua Univ, Dept Phys, State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Histol & Embryol, Beijing 100871, Peoples R China
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Tian, N.,Zhang, L.,Zheng, J. H.,et al. Three-dimensional quantitative analysis of chromosomes in the oocytes of aging mice during meiosis I in vitro[J]. THERIOGENOLOGY,2013,79(2):249-256.
APA Tian, N.,Zhang, L.,Zheng, J. H.,Lv, D. Y.,Li, Y.,&Ma, W. Y..(2013).Three-dimensional quantitative analysis of chromosomes in the oocytes of aging mice during meiosis I in vitro.THERIOGENOLOGY,79(2),249-256.
MLA Tian, N.,et al."Three-dimensional quantitative analysis of chromosomes in the oocytes of aging mice during meiosis I in vitro".THERIOGENOLOGY 79.2(2013):249-256.
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