IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
hALP, A Novel Transcriptional U Three Protein (t-UTP), Activates RNA Polymerase I Transcription by Binding and Acetylating the Upstream Binding Factor (UBF)
Kong, Ruirui2,3; Zhang, Liangliang1,4; Hu, Lelin1,4; Peng, Qunhui2,3; Han, Wei1; Du, Xiaojuan1,4; Ke, Yang1,2,3
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2011-03-04
DOI10.1074/jbc.M110.173393
286期:9页:7139-7148
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
资助者National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program) ; National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program) ; National Basic Research Program of China (973 Program)
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]PRE-RIBOSOMAL-RNA ; NUCLEOLAR TRANSCRIPTION ; RDNA TRANSCRIPTION ; GENE-TRANSCRIPTION ; COMPLEX SL1 ; HUMAN-CELLS ; PHOSPHORYLATION ; PROMOTER ; RECRUITMENT ; SUBUNIT
英文摘要

Transcription of ribosome RNA precursor (pre-rRNA) and pre-rRNA processing are coordinated by a subset of U three proteins (UTPs) known as transcriptional UTPs (t-UTPs), which participate in pre-rRNA transcription in addition to participation in 18 S rRNA processing. However, the mechanism by which t-UTPs function in pre-rRNA transcription remains undetermined. In the present study, we identified hALP, a histone acetyl-transferase as a novel t-UTP. We first showed that hALP is nucleolar, and is associated with U3 snoRNA and required for 18 S rRNA processing. Moreover, depletion of hALP resulted in a decreased level of 47 S pre-rRNA. Ectopic expression of hALP activated the rDNA promoter luciferase reporter and knockdown of hALP inhibited the reporter. In addition, hALP bound rDNA. Taken together these data identify hALP as a novel t-UTP. Immunoprecipitation and GST pulldown experiments showed that hALP binds the upstream binding factor (UBF) in vivo and in vitro. It is of importance that hALP acetylated UBF depending on HAT in vivo, and hALP but not hALP (Delta HAT) facilitated the nuclear translocation of the RNA polymerase I (Pol I)-associated factor 53 (PAF53) from the cytoplasm and promoted the association of UBF with PAF53. Thus, we provide a mechanism in which a novel t-UTP activates Pol I transcription by binding and acetylating UBF.

语种英语
所属项目编号30571038 ; 30771224 ; 2008AA02Z131 ; 2006AA02A402 ; 2010CB529303
资助者National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program) ; National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program) ; National Basic Research Program of China (973 Program)
WOS记录号WOS:000287737300031
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65542
Collection北京大学临床肿瘤学院
作者单位1.Peking Univ, Canc Res Ctr, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Dept Genet, Beijing 100142, Peoples R China
4.Peking Univ, Dept Cell Biol, Hlth Sci Ctr, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Kong, Ruirui,Zhang, Liangliang,Hu, Lelin,et al. hALP, A Novel Transcriptional U Three Protein (t-UTP), Activates RNA Polymerase I Transcription by Binding and Acetylating the Upstream Binding Factor (UBF)[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2011,286(9):7139-7148.
APA Kong, Ruirui.,Zhang, Liangliang.,Hu, Lelin.,Peng, Qunhui.,Han, Wei.,...&Ke, Yang.(2011).hALP, A Novel Transcriptional U Three Protein (t-UTP), Activates RNA Polymerase I Transcription by Binding and Acetylating the Upstream Binding Factor (UBF).JOURNAL OF BIOLOGICAL CHEMISTRY,286(9),7139-7148.
MLA Kong, Ruirui,et al."hALP, A Novel Transcriptional U Three Protein (t-UTP), Activates RNA Polymerase I Transcription by Binding and Acetylating the Upstream Binding Factor (UBF)".JOURNAL OF BIOLOGICAL CHEMISTRY 286.9(2011):7139-7148.
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