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学科主题: 临床医学
题名:
hALP, A Novel Transcriptional U Three Protein (t-UTP), Activates RNA Polymerase I Transcription by Binding and Acetylating the Upstream Binding Factor (UBF)
作者: Kong, Ruirui2,3; Zhang, Liangliang1,4; Hu, Lelin1,4; Peng, Qunhui2,3; Han, Wei1; Du, Xiaojuan1,4; Ke, Yang1,2,3
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
发表日期: 2011-03-04
DOI: 10.1074/jbc.M110.173393
卷: 286, 期:9, 页:7139-7148
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: PRE-RIBOSOMAL-RNA ; NUCLEOLAR TRANSCRIPTION ; RDNA TRANSCRIPTION ; GENE-TRANSCRIPTION ; COMPLEX SL1 ; HUMAN-CELLS ; PHOSPHORYLATION ; PROMOTER ; RECRUITMENT ; SUBUNIT
英文摘要:

Transcription of ribosome RNA precursor (pre-rRNA) and pre-rRNA processing are coordinated by a subset of U three proteins (UTPs) known as transcriptional UTPs (t-UTPs), which participate in pre-rRNA transcription in addition to participation in 18 S rRNA processing. However, the mechanism by which t-UTPs function in pre-rRNA transcription remains undetermined. In the present study, we identified hALP, a histone acetyl-transferase as a novel t-UTP. We first showed that hALP is nucleolar, and is associated with U3 snoRNA and required for 18 S rRNA processing. Moreover, depletion of hALP resulted in a decreased level of 47 S pre-rRNA. Ectopic expression of hALP activated the rDNA promoter luciferase reporter and knockdown of hALP inhibited the reporter. In addition, hALP bound rDNA. Taken together these data identify hALP as a novel t-UTP. Immunoprecipitation and GST pulldown experiments showed that hALP binds the upstream binding factor (UBF) in vivo and in vitro. It is of importance that hALP acetylated UBF depending on HAT in vivo, and hALP but not hALP (Delta HAT) facilitated the nuclear translocation of the RNA polymerase I (Pol I)-associated factor 53 (PAF53) from the cytoplasm and promoted the association of UBF with PAF53. Thus, we provide a mechanism in which a novel t-UTP activates Pol I transcription by binding and acetylating UBF.

语种: 英语
所属项目编号: 30571038 ; 30771224 ; 2008AA02Z131 ; 2006AA02A402 ; 2010CB529303
项目资助者: National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program) ; National Basic Research Program of China (973 Program)
WOS记录号: WOS:000287737300031
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65542
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Canc Res Ctr, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Dept Genet, Beijing 100142, Peoples R China
4.Peking Univ, Dept Cell Biol, Hlth Sci Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Kong, Ruirui,Zhang, Liangliang,Hu, Lelin,et al. hALP, A Novel Transcriptional U Three Protein (t-UTP), Activates RNA Polymerase I Transcription by Binding and Acetylating the Upstream Binding Factor (UBF)[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2011,286(9):7139-7148.
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