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学科主题: 临床医学
题名:
Effects of adrenomedullin on cell proliferation in rat adventitia induced by aldosterone
作者: Jiang, W; Yang, JH; Pan, CS; Qi, YF; Pang, YZ; Tang, CS
关键词: aldosterone ; adrenomedullin ; proliferation ; adventitia
刊名: JOURNAL OF HYPERTENSION
发表日期: 2004-10-01
卷: 22, 期:10, 页:1953-1961
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Peripheral Vascular Disease
研究领域[WOS]: Cardiovascular System & Cardiology
关键词[WOS]: SMOOTH-MUSCLE-CELLS ; VASCULAR ALDOSTERONE ; IN-VITRO ; PEPTIDE ; RECEPTOR ; GENE ; SECRETION ; FIBROSIS ; TARGET ; PROADRENOMEDULLIN
英文摘要:

Objective Aldosterone is involved in cardiovascular diseases such as hypertension and heart failure by inducing sodium retention and vascular remodeling, which is characterized by fibroblast proliferation and migration in adventitia. It is well known that aldosterone stimulates vascular smooth muscle cells and fibroblasts to produce and secrete adrenomedullin (ADM), a multiple functional peptide with an important cytoprotective effect against cardiovascular damage. We examined the effect of aldosterone on ADM production and secretion and its mRNA expression in rat aortic adventitia to study the paracrine/autocrine interaction between endogenous ADM and aldosterone.

Methods ADM produced and secreted from adventitia stimulated by aldosterone in the absence or presence of spironolactone, RU486 or spironolactone together with RU486 were detected by radioimmunoassay, proliferation in adventitia cells was evaluated by the level of [H-3]-thymine incorporation, and preproADM gene expression was measured by semi-quantitative reverse transcriptase polymerase chain reaction.

Results Adventitial ADM secretion and mRNA expression stimulated by aldosterone were concentration-dependent as was the inhibitive effect of ADM on aldosterone-induced proliferation. The induction of aldosterone in ADM secretion was mediated by mineralocorticoid receptor. Antagonists of specific receptors of calcitonin gene-related peptide (CGRP) receptor type 1 and ADM both potentiated the proliferation effect induced by aldosterone; and thiorphan, an inhibitor of the enzyme for ADM degradation, inhibited the adventitial [H-3]-thymine incorporation induced by aldosterone. ADM inhibited the activity of extracellular signal related kinase (ERK) stimulated by aldosterone.

Conclusion Aldosterone stimulates adventitia to produce and secrete ADM, which in turn, antagonizes the aldosterone-induced proliferation in adventitia.

语种: 英语
WOS记录号: WOS:000224364900018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65544
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ First Hosp, Inst Cardiovasc Dis, Beijing 100034, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100083, Peoples R China
3.Minist Hlth Cardiovasc Mol Biol & Regulatory Pept, Reference Lab, Beijing 100083, Peoples R China

Recommended Citation:
Jiang, W,Yang, JH,Pan, CS,et al. Effects of adrenomedullin on cell proliferation in rat adventitia induced by aldosterone[J]. JOURNAL OF HYPERTENSION,2004,22(10):1953-1961.
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