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学科主题临床医学
Effects of adrenomedullin on cell proliferation in rat adventitia induced by aldosterone
Jiang, W; Yang, JH; Pan, CS; Qi, YF; Pang, YZ; Tang, CS
关键词aldosterone adrenomedullin proliferation adventitia
刊名JOURNAL OF HYPERTENSION
2004-10-01
22期:10页:1953-1961
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Peripheral Vascular Disease
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]SMOOTH-MUSCLE-CELLS ; VASCULAR ALDOSTERONE ; IN-VITRO ; PEPTIDE ; RECEPTOR ; GENE ; SECRETION ; FIBROSIS ; TARGET ; PROADRENOMEDULLIN
英文摘要

Objective Aldosterone is involved in cardiovascular diseases such as hypertension and heart failure by inducing sodium retention and vascular remodeling, which is characterized by fibroblast proliferation and migration in adventitia. It is well known that aldosterone stimulates vascular smooth muscle cells and fibroblasts to produce and secrete adrenomedullin (ADM), a multiple functional peptide with an important cytoprotective effect against cardiovascular damage. We examined the effect of aldosterone on ADM production and secretion and its mRNA expression in rat aortic adventitia to study the paracrine/autocrine interaction between endogenous ADM and aldosterone.

Methods ADM produced and secreted from adventitia stimulated by aldosterone in the absence or presence of spironolactone, RU486 or spironolactone together with RU486 were detected by radioimmunoassay, proliferation in adventitia cells was evaluated by the level of [H-3]-thymine incorporation, and preproADM gene expression was measured by semi-quantitative reverse transcriptase polymerase chain reaction.

Results Adventitial ADM secretion and mRNA expression stimulated by aldosterone were concentration-dependent as was the inhibitive effect of ADM on aldosterone-induced proliferation. The induction of aldosterone in ADM secretion was mediated by mineralocorticoid receptor. Antagonists of specific receptors of calcitonin gene-related peptide (CGRP) receptor type 1 and ADM both potentiated the proliferation effect induced by aldosterone; and thiorphan, an inhibitor of the enzyme for ADM degradation, inhibited the adventitial [H-3]-thymine incorporation induced by aldosterone. ADM inhibited the activity of extracellular signal related kinase (ERK) stimulated by aldosterone.

Conclusion Aldosterone stimulates adventitia to produce and secrete ADM, which in turn, antagonizes the aldosterone-induced proliferation in adventitia.

语种英语
WOS记录号WOS:000224364900018
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65544
专题北京大学第一临床医学院_心血管内科
作者单位1.Peking Univ First Hosp, Inst Cardiovasc Dis, Beijing 100034, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100083, Peoples R China
3.Minist Hlth Cardiovasc Mol Biol & Regulatory Pept, Reference Lab, Beijing 100083, Peoples R China
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GB/T 7714
Jiang, W,Yang, JH,Pan, CS,et al. Effects of adrenomedullin on cell proliferation in rat adventitia induced by aldosterone[J]. JOURNAL OF HYPERTENSION,2004,22(10):1953-1961.
APA Jiang, W,Yang, JH,Pan, CS,Qi, YF,Pang, YZ,&Tang, CS.(2004).Effects of adrenomedullin on cell proliferation in rat adventitia induced by aldosterone.JOURNAL OF HYPERTENSION,22(10),1953-1961.
MLA Jiang, W,et al."Effects of adrenomedullin on cell proliferation in rat adventitia induced by aldosterone".JOURNAL OF HYPERTENSION 22.10(2004):1953-1961.
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