IR@PKUHSC  > 北京大学基础医学院  > 心血管所
学科主题基础医学
Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance
Dou, Dou1,2; Ma, Huijuan1; Zheng, Xiaoxu1; Ying, Lei1; Guo, Yixuan1; Yu, Xiaoxing1; Gao, Yuansheng1,2
关键词PKG Myosin light chain phosphatase Proteasome
刊名CARDIOVASCULAR RESEARCH
2010-04-01
DOI10.1093/cvr/cvp376
86期:1页:151-159
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]DEPENDENT PROTEIN-KINASE ; LIGHT-CHAIN PHOSPHATASE ; MITOCHONDRIAL ALDEHYDE DEHYDROGENASE ; SMOOTH-MUSCLE RELAXATION ; NITRIC-OXIDE ; MYOSIN PHOSPHATASE ; CYCLIC-GMP ; NITROGLYCERIN TOLERANCE ; GLYCERYL TRINITRATE ; GUANYLATE-CYCLASE
英文摘要

A depressed cGMP-dependent protein kinase (PKG) activity is implicated in nitrate tolerance. The present study determines whether the leucine zipper-positive (LZ+) isoform of myosin phosphatase target subunit 1 (MYPT1), a key target protein for PKG actions, is involved in the development of nitrate tolerance.

Nitrate tolerance in in vitro preparations was obtained by a 24 h incubation with nitroglycerin (NTG). Nitrate tolerance in in vivo preparations was obtained by subcutaneous injection of mice with NTG, and the aortas were used. Protein levels of total MYPT1, MYPT1 (LZ+), PP1C delta, myosin light chain (MLC), and phosphorylated MLC were determined by Western blot analysis. Isometric vessel tension was determined by an organ chamber technique. Protein levels of MYPT1 (LZ+), but not of PP1C delta, were significantly reduced in in vitro and in vivo nitrate-tolerant arteries. The decrease in the MYPT1 (LZ+) protein level of coronary artery was also induced by a nitric oxide donor and a cGMP analogue, which was prevented by the inhibitors of soluble guanylyl cyclase and PKG. The decrease in MYPT1 (LZ+) protein levels was not affected by the inhibitor of protein synthesis, but was prevented by the inhibitors of proteasomes. The diminished inhibition of dephosphorylation of MLC as well as the attenuated relaxation of porcine coronary artery and mouse aorta to NTG was improved by proteasome inhibitors.

This study demonstrates that a reduction in the protein level of MYPT1 (LZ+) is involved in nitrate tolerance. This may result in part from a proteasome-dependent degradation of MYPT1 (LZ+).

语种英语
WOS记录号WOS:000275566400020
项目编号30770789 ; 30870938 ; 2006CB503802
资助机构National Natural Science Foundation of China ; Major National Basic Research Program of China
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65548
专题北京大学基础医学院_心血管所
北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
2.Peking Univ, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Dou, Dou,Ma, Huijuan,Zheng, Xiaoxu,et al. Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance[J]. CARDIOVASCULAR RESEARCH,2010,86(1):151-159.
APA Dou, Dou.,Ma, Huijuan.,Zheng, Xiaoxu.,Ying, Lei.,Guo, Yixuan.,...&Gao, Yuansheng.(2010).Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance.CARDIOVASCULAR RESEARCH,86(1),151-159.
MLA Dou, Dou,et al."Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance".CARDIOVASCULAR RESEARCH 86.1(2010):151-159.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
151.full.pdf(451KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Dou, Dou]的文章
[Ma, Huijuan]的文章
[Zheng, Xiaoxu]的文章
百度学术
百度学术中相似的文章
[Dou, Dou]的文章
[Ma, Huijuan]的文章
[Zheng, Xiaoxu]的文章
必应学术
必应学术中相似的文章
[Dou, Dou]的文章
[Ma, Huijuan]的文章
[Zheng, Xiaoxu]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 151.full.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。