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学科主题临床医学
MicroRNA Expression Signatures of Bladder Cancer Revealed by Deep Sequencing
Han, Yonghua1,2; Chen, Jiahao3; Zhao, Xiaokun4; Liang, Chaozhao5; Wang, Yong2,6; Sun, Liang2,6; Jiang, Zhimao2,6; Zhang, Zhongfu2,6; Yang, Ruilin2,7; Chen, Jing2,6; Li, Zesong2,6; Tang, Aifa2,6; Li, Xianxin1,6; Ye, Jiongxian2,6; Guan, Zhichen1,6; Gui, Yaoting2,6; Cai, Zhiming1,2,8
刊名PLOS ONE
2011-03-28
DOI10.1371/journal.pone.0018286
6期:3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]MESENCHYMAL TRANSITION ; IDENTIFICATION ; CARCINOMA ; DISEASE ; GENES
英文摘要

Background: MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression. They are aberrantly expressed in many types of cancers. In this study, we determined the genome-wide miRNA profiles in bladder urothelial carcinoma by deep sequencing.

Methodology/Principal Findings: We detected 656 differentially expressed known human miRNAs and miRNA antisense sequences (miRNA*s) in nine bladder urothelial carcinoma patients by deep sequencing. Many miRNAs and miRNA*s were significantly upregulated or downregulated in bladder urothelial carcinoma compared to matched histologically normal urothelium. hsa-miR-96 was the most significantly upregulated miRNA and hsa-miR-490-5p was the most significantly downregulated one. Upregulated miRNAs were more common than downregulated ones. The hsa-miR-183, hsa-miR-200b similar to 429, hsa-miR-200c similar to 141 and hsa-miR-17 similar to 92 clusters were significantly upregulated. The hsa-miR-143 similar to 145 cluster was significantly downregulated. hsa-miR-182, hsa-miR-183, hsa-miR-200a, hsa-miR-143 and hsa-miR-195 were evaluated by Real-Time qPCR in a total of fifty-one bladder urothelial carcinoma patients. They were aberrantly expressed in bladder urothelial carcinoma compared to matched histologically normal urothelium (p < 0.001 for each miRNA).

Conclusions/Significance: To date, this is the first study to determine genome-wide miRNA expression patterns in human bladder urothelial carcinoma by deep sequencing. We found that a collection of miRNAs were aberrantly expressed in bladder urothelial carcinoma compared to matched histologically normal urothelium, suggesting that they might play roles as oncogenes or tumor suppressors in the development and/or progression of this cancer. Our data provide novel insights into cancer biology.

语种英语
WOS记录号WOS:000289053800037
引用统计
被引频次:156[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65555
专题北京大学深圳医院_泌尿外科
北京大学第二临床医学院_麻醉科
作者单位1.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen, Peoples R China
2.Peking Univ, Shenzhen Hosp, Guangdong Key Lab Male Reprod Med & Genet, Shenzhen, Peoples R China
3.Beijing Genom Inst Shenzhen, Shenzhen, Peoples R China
4.Cent S Univ, Xiangya Hosp 2, Dept Urol, Changsha, Hunan, Peoples R China
5.Shantou Univ, Coll Med, Shantou, Peoples R China
6.Anhui Med Univ, Affiliated Hosp 1, Dept Urol, Hefei, Peoples R China
7.Shenzhen PKU HKUST Med Ctr, Inst Urol, Shenzhen, Peoples R China
8.Second Peoples Hosp Shenzhen, Dept Urol, Shenzhen, Peoples R China
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GB/T 7714
Han, Yonghua,Chen, Jiahao,Zhao, Xiaokun,et al. MicroRNA Expression Signatures of Bladder Cancer Revealed by Deep Sequencing[J]. PLOS ONE,2011,6(3).
APA Han, Yonghua.,Chen, Jiahao.,Zhao, Xiaokun.,Liang, Chaozhao.,Wang, Yong.,...&Cai, Zhiming.(2011).MicroRNA Expression Signatures of Bladder Cancer Revealed by Deep Sequencing.PLOS ONE,6(3).
MLA Han, Yonghua,et al."MicroRNA Expression Signatures of Bladder Cancer Revealed by Deep Sequencing".PLOS ONE 6.3(2011).
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