|MicroRNA Expression Signatures of Bladder Cancer Revealed by Deep Sequencing|
|Han, Yonghua1,2; Chen, Jiahao3; Zhao, Xiaokun4; Liang, Chaozhao5; Wang, Yong2,6; Sun, Liang2,6; Jiang, Zhimao2,6; Zhang, Zhongfu2,6; Yang, Ruilin2,7; Chen, Jing2,6; Li, Zesong2,6; Tang, Aifa2,6; Li, Xianxin1,6; Ye, Jiongxian2,6; Guan, Zhichen1,6; Gui, Yaoting2,6; Cai, Zhiming1,2,8|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Science & Technology - Other Topics|
|关键词[WOS]||MESENCHYMAL TRANSITION ; IDENTIFICATION ; CARCINOMA ; DISEASE ; GENES|
Background: MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression. They are aberrantly expressed in many types of cancers. In this study, we determined the genome-wide miRNA profiles in bladder urothelial carcinoma by deep sequencing.
Methodology/Principal Findings: We detected 656 differentially expressed known human miRNAs and miRNA antisense sequences (miRNA*s) in nine bladder urothelial carcinoma patients by deep sequencing. Many miRNAs and miRNA*s were significantly upregulated or downregulated in bladder urothelial carcinoma compared to matched histologically normal urothelium. hsa-miR-96 was the most significantly upregulated miRNA and hsa-miR-490-5p was the most significantly downregulated one. Upregulated miRNAs were more common than downregulated ones. The hsa-miR-183, hsa-miR-200b similar to 429, hsa-miR-200c similar to 141 and hsa-miR-17 similar to 92 clusters were significantly upregulated. The hsa-miR-143 similar to 145 cluster was significantly downregulated. hsa-miR-182, hsa-miR-183, hsa-miR-200a, hsa-miR-143 and hsa-miR-195 were evaluated by Real-Time qPCR in a total of fifty-one bladder urothelial carcinoma patients. They were aberrantly expressed in bladder urothelial carcinoma compared to matched histologically normal urothelium (p < 0.001 for each miRNA).
Conclusions/Significance: To date, this is the first study to determine genome-wide miRNA expression patterns in human bladder urothelial carcinoma by deep sequencing. We found that a collection of miRNAs were aberrantly expressed in bladder urothelial carcinoma compared to matched histologically normal urothelium, suggesting that they might play roles as oncogenes or tumor suppressors in the development and/or progression of this cancer. Our data provide novel insights into cancer biology.
|项目编号||2006AA02A302 ; 2009AA022707 ; CXB200903090055A ; CXC201005260001A|
|资助机构||National High Technology Research and Development Program of China ; Promotion Program for Shenzhen Key Laboratory, Shenzhen, China ; Bank of Clinical Data of Major Diseases and Biological Specimens of Shenzhen|
|作者单位||1.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen, Peoples R China|
2.Peking Univ, Shenzhen Hosp, Guangdong Key Lab Male Reprod Med & Genet, Shenzhen, Peoples R China
3.Beijing Genom Inst Shenzhen, Shenzhen, Peoples R China
4.Cent S Univ, Xiangya Hosp 2, Dept Urol, Changsha, Hunan, Peoples R China
5.Shantou Univ, Coll Med, Shantou, Peoples R China
6.Anhui Med Univ, Affiliated Hosp 1, Dept Urol, Hefei, Peoples R China
7.Shenzhen PKU HKUST Med Ctr, Inst Urol, Shenzhen, Peoples R China
8.Second Peoples Hosp Shenzhen, Dept Urol, Shenzhen, Peoples R China
|Han, Yonghua,Chen, Jiahao,Zhao, Xiaokun,et al. MicroRNA Expression Signatures of Bladder Cancer Revealed by Deep Sequencing[J]. PLOS ONE,2011,6(3).|
|APA||Han, Yonghua.,Chen, Jiahao.,Zhao, Xiaokun.,Liang, Chaozhao.,Wang, Yong.,...&Cai, Zhiming.(2011).MicroRNA Expression Signatures of Bladder Cancer Revealed by Deep Sequencing.PLOS ONE,6(3).|
|MLA||Han, Yonghua,et al."MicroRNA Expression Signatures of Bladder Cancer Revealed by Deep Sequencing".PLOS ONE 6.3(2011).|