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学科主题口腔医学
Secreted phosphoprotein 24 kD inhibits nerve root inflammation induced by bone morphogenetic protein-2
Tian, Haijun1,2; Li, Chen-Shuang3; Scott, Trevor P.2; Montgomery, Scott R.2; Phan, Kevin2; Lao, Lifeng2; Zhang, Wei2; Li, Yawei2; Hayashi, Tetsuo2; Takahashi, Shinji2; Alobaidaan, Raed2; Ruangchainikom, Monchai2; Zhao, Ke-Wei4; Brochmann, Elsa J.4,5,6; Murray, Samuel S.4,5,6; Wang, Jeffrey C.7; Daubs, Michael D.8
关键词Secreted phosphoprotein 24 kD Bone morphogenetic protein-2 Nerve root inflammation Spine surgery Posterior lumbar fusion Complications
刊名SPINE JOURNAL
2015-02-01
DOI10.1016/j.spinee.2014.09.021
15期:2页:314-321
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Clinical Neurology ; Orthopedics
资助者AO Spine ; Dept. VA ; AO Foundation ; Cervical Spine Research Society ; AO Spine of North America ; AO Spine ; Dept. VA ; AO Foundation ; Cervical Spine Research Society ; AO Spine of North America
研究领域[WOS]Neurosciences & Neurology ; Orthopedics
关键词[WOS]GENE-RELATED PEPTIDE ; BINDING PEPTIDE ; SENSORY NEURONS ; RODENT MODEL ; SURGERY ; FUSION ; ARTHRODESIS ; EXPRESSION ; RECOVERY ; INJURY
英文摘要

BACKGROUND CONTEXT: Bone morphogenetic protein-2 (BMP-2) has been used to successfully promote spine fusion, but side-effects including nerve inflammation have been observed.

PURPOSE: To investigate the direct neurotoxic effects of BMP-2 and test the hypotheses that the use of BMP binding proteins, such as secreted phosphoprotein 24 kD (Spp24), can reduce or eliminate these effects.

STUDY DESIGN: In vitro experiments and in vivo analysis in a rodent model.

METHODS: In vitro, dorsal root ganglion cells were cultured in the presence of BMP-2 with and without Spp24 and calcitonin gene-related peptide and Substance P, markers of neuroinflammation, were measured by immunohistochemistry. In vivo, rats underwent a left-sided laminotomy at L5 to expose the S1 nerve root and were randomized into four different groups according to the intervention at the laminotomy site: collagen sponge only (no BMP-2 or Spp24), BMP-2 in a collagen sponge only, BMP-2 in a collagen sponge+an empty collagen sponge to act as a barrier, and BMP-2 in a collagen sponge+Spp24 in a collagen sponge to act as a barrier. Functional evaluation was done using the Basso, Beattie, and Bresnahan scale and immunohistochemical analyses were performed using calcitonin gene-related peptide and Substance P staining.

RESULTS: The neuroinflammatory effects of BMP-2 in vitro were ameliorated by the addition of Spp24. Similarly, in vivo, Spp24 reduced the expression of markers on neuroinflammation in animals treated with BMP-2 and also improved the function after BMP-2 administration.

CONCLUSIONS: These results confirm that BMP binding proteins have great potential as adjuvant therapies to limit BMP-2 related side-effects in spine surgery. (C) 2015 Elsevier Inc. All rights reserved.

语种英语
资助者AO Spine ; Dept. VA ; AO Foundation ; Cervical Spine Research Society ; AO Spine of North America ; AO Spine ; Dept. VA ; AO Foundation ; Cervical Spine Research Society ; AO Spine of North America
WOS记录号WOS:000348045900019
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65558
专题北京大学口腔医学院_牙周科
作者单位1.Second Mil Med Univ, Shanghai Changzheng Hosp, Dept Orthopaed Surg, Shanghai 200003, Peoples R China
2.Univ Calif Los Angeles, Dept Orthopaed Surg, Los Angeles, CA 90024 USA
3.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100081, Peoples R China
4.VA Greater Los Angeles Healthcare Syst, Res Serv, North Hills, CA 91343 USA
5.VA Greater Los Angeles Healthcare Syst, Geriatr Res Educ & Clin Ctr, North Hills, CA 91343 USA
6.Univ Calif Los Angeles, Dept Med, Los Angeles, CA 90024 USA
7.Univ So Calif, Keck Sch Med, Dept Orthopaed Surg, Los Angeles, CA 90033 USA
8.Univ Nevada, Sch Med, Dept Surg, Div Orthopaed Surg, Las Vegas, NV 89102 USA
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Tian, Haijun,Li, Chen-Shuang,Scott, Trevor P.,et al. Secreted phosphoprotein 24 kD inhibits nerve root inflammation induced by bone morphogenetic protein-2[J]. SPINE JOURNAL,2015,15(2):314-321.
APA Tian, Haijun.,Li, Chen-Shuang.,Scott, Trevor P..,Montgomery, Scott R..,Phan, Kevin.,...&Daubs, Michael D..(2015).Secreted phosphoprotein 24 kD inhibits nerve root inflammation induced by bone morphogenetic protein-2.SPINE JOURNAL,15(2),314-321.
MLA Tian, Haijun,et al."Secreted phosphoprotein 24 kD inhibits nerve root inflammation induced by bone morphogenetic protein-2".SPINE JOURNAL 15.2(2015):314-321.
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