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学科主题: 临床医学
题名:
Detection of frameshift mutations of RIZ in gastric cancers with microsatellite instability
作者: Pan, Kai-Feng1; Lu, You-Yong2; Liu, Wan-Guo3; Zhang, Lian1; You, Wei-Cheng1
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2004-09-15
卷: 10, 期:18, 页:2719-2722
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
英文摘要:

AIM: To study the frameshift mutations of the retinoblastoma protein-interacting zinc finger gene RIZ in gastric cancer with microsatellite instability, and to identify two coding polyadenosine tracts of RIZ.

METHODS: Frameshift mutations at (A)(8) and (A)(9) tracts of RIZ were detected in 70 human gastric cancer (HGC) specimens by DHPLC and DNA sequencing. Microsatellite instability (MSI) status was assessed by two mononucleotide markers, BAT26 and BAT25, by means of denaturing high-performance liquid chromatography (DHPLC).

RESULTS: In 70 HGC samples, 8 (11.4%) were found positive for instabilities at BAT26 and BAT25. In 7 of the 8 cases with instabilities at both BAT26 and BAT25 (MSI-H), 1 was unstable at BAT26 but stable at BAT25. Frameshift mutations were identified in 4 (57.1%) of the 7 samples with MSI-H in the (A) 9 tract of RIZ without mutations in the (A)(8) tract. In contrast, frameshift mutations were found in neither of the polyadenosine tracts in 63 samples of MSI-L or MSI stable tumors. Pro704 LOH detection in 4 cases with frameshift mutations did not find LOH in these cases.

CONCLUSION: Frameshift mutations of RIZ may play an important role in gastric cancers with MSI.

语种: 英语
所属项目编号: G1998051203 ; H020920030130
项目资助者: Ministry of Science and Technology of China ; Beijing Municipal Commission Foundation for Science and Technology
WOS记录号: WOS:000208097100021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65576
Appears in Collections:北京大学临床肿瘤学院_流行病学研究室_期刊论文

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作者单位: 1.Peking Univ, Sch Oncol, Dept Canc Epidemiol, Beijing Inst Canc Res,Beijing Canc Hosp, Beijing 100034, Peoples R China
2.Peking Univ, Sch Oncol, Beijing Lab Mol Oncol, Beijing Inst Canc Res,Beijing Canc Hosp, Beijing 100034, Peoples R China
3.Mayo Clin & Mayo Grad Sch Med, Mayo Clin, Div Expt Pathol, Dept Lab Med & Pathol, Rochester, MN 55905 USA

Recommended Citation:
Pan, Kai-Feng,Lu, You-Yong,Liu, Wan-Guo,et al. Detection of frameshift mutations of RIZ in gastric cancers with microsatellite instability[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(18):2719-2722.
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