IR@PKUHSC  > 北京大学第三临床医学院  > 骨科
学科主题临床医学
Tissue engineered cartilage from hTGF beta 2 transduced human adipose derived stem cells seeded in PLGA/alginate compound in vitro and in vivo
Jin, Xiao Bing1; Sun, Yong Sheng1; Zhang, Ke1; Wang, Jing2; Shi, Tai Ping3; Ju, Xiao Dong1; Lou, Si Quan1
关键词human adipose derived stem cells (hASCs) transforming growth factor beta 2 (TGF beta 2) adenovirus cartilage tissue engineering
刊名JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
2008-09-15
DOI10.1002/jbm.a.31656
86A期:4页:1077-1087
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
资助者Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]ALGINATE-CHONDROCYTE CONSTRUCTS ; MESENCHYMAL PROGENITOR CELLS ; MARROW STROMAL CELLS ; BONE-MARROW ; ARTICULAR-CARTILAGE ; GENE-EXPRESSION ; CHONDROGENIC DIFFERENTIATION ; FIBER STRUCTURES ; HUMAN FAT ; CULTURE
英文摘要

Chondrogenic potential of human adipose derived stem cells (hASCs) makes them a possible source of seeding cells for cartilage tissue engineering. We aim to examine the chondrogenic differentiation of human transforming growth factor beta 2 (hTGF beta 2) transduced hASCs seeded in three-dimensional scaffold ill vitro and ill vivo. In this study, hASCs were isolated from human subcutaneous adipose tissue and transduced with a replication deficient adenovirus carrying hTGF beta 2 (Ad5-hTGF beta 2), and then the transduced cells were seeded and cultured in PLGA/alginate compounds. RT-PCR analysis revealed that Ad5-hTGF beta 2 transduced hASCs produced aggrecan and collagen type II after 7-day induction ill vitro and continued throughout the culture period; this was also demonstrated by the positive staining of Alcian blue and immunohistochemistry for collagen type It. For ill vivo study, Ad5-hTGF beta 2 transduced hASCs seeded in PLGA/alginate compounds were implanted in subcutaneous pockets of nude mice; after 12 weeks, the implants were harvested and examined by haematoxylin and eosin staining, AB-PAS staining, and immunohistochemical analysis, and the results demonstrated the formation of cartilage tissue. As a control, all these were not observed in the constructs with Ad5-EGFP transduced hASCs. In conclusion, our study demonstrates that adenovirus-mediated hTGF beta 2 gene transfer is able to induce the hASCs into chondrogenic lineage both ill vitro and in vivo. Ads-hTGF beta 2 transduced hASCs combined with three-dimensional PLGA/alginate compound may be a viable method in treating injuries of cartilage. (c) 2007 Wiley Periodicals, Inc.

语种英语
所属项目编号30371431 ; 30500254
资助者Chinese National Natural Science Foundation ; Chinese National Natural Science Foundation
WOS记录号WOS:000258637100026
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65577
Collection北京大学第三临床医学院_骨科
作者单位1.Chinese Natl Human Genome Ctr, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100871, Peoples R China
3.Fourth Mil Med Univ, Coll Stomatol, Dept Dent, Xian 710032, Peoples R China
Recommended Citation
GB/T 7714
Jin, Xiao Bing,Sun, Yong Sheng,Zhang, Ke,et al. Tissue engineered cartilage from hTGF beta 2 transduced human adipose derived stem cells seeded in PLGA/alginate compound in vitro and in vivo[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2008,86A(4):1077-1087.
APA Jin, Xiao Bing.,Sun, Yong Sheng.,Zhang, Ke.,Wang, Jing.,Shi, Tai Ping.,...&Lou, Si Quan.(2008).Tissue engineered cartilage from hTGF beta 2 transduced human adipose derived stem cells seeded in PLGA/alginate compound in vitro and in vivo.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,86A(4),1077-1087.
MLA Jin, Xiao Bing,et al."Tissue engineered cartilage from hTGF beta 2 transduced human adipose derived stem cells seeded in PLGA/alginate compound in vitro and in vivo".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 86A.4(2008):1077-1087.
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