北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 骨科  > 期刊论文
学科主题: 临床医学
题名:
Tissue engineered cartilage from hTGF beta 2 transduced human adipose derived stem cells seeded in PLGA/alginate compound in vitro and in vivo
作者: Jin, Xiao Bing1; Sun, Yong Sheng1; Zhang, Ke1; Wang, Jing2; Shi, Tai Ping3; Ju, Xiao Dong1; Lou, Si Quan1
关键词: human adipose derived stem cells (hASCs) ; transforming growth factor beta 2 (TGF beta 2) ; adenovirus ; cartilage ; tissue engineering
刊名: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
发表日期: 2008-09-15
DOI: 10.1002/jbm.a.31656
卷: 86A, 期:4, 页:1077-1087
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: ALGINATE-CHONDROCYTE CONSTRUCTS ; MESENCHYMAL PROGENITOR CELLS ; MARROW STROMAL CELLS ; BONE-MARROW ; ARTICULAR-CARTILAGE ; GENE-EXPRESSION ; CHONDROGENIC DIFFERENTIATION ; FIBER STRUCTURES ; HUMAN FAT ; CULTURE
英文摘要:

Chondrogenic potential of human adipose derived stem cells (hASCs) makes them a possible source of seeding cells for cartilage tissue engineering. We aim to examine the chondrogenic differentiation of human transforming growth factor beta 2 (hTGF beta 2) transduced hASCs seeded in three-dimensional scaffold ill vitro and ill vivo. In this study, hASCs were isolated from human subcutaneous adipose tissue and transduced with a replication deficient adenovirus carrying hTGF beta 2 (Ad5-hTGF beta 2), and then the transduced cells were seeded and cultured in PLGA/alginate compounds. RT-PCR analysis revealed that Ad5-hTGF beta 2 transduced hASCs produced aggrecan and collagen type II after 7-day induction ill vitro and continued throughout the culture period; this was also demonstrated by the positive staining of Alcian blue and immunohistochemistry for collagen type It. For ill vivo study, Ad5-hTGF beta 2 transduced hASCs seeded in PLGA/alginate compounds were implanted in subcutaneous pockets of nude mice; after 12 weeks, the implants were harvested and examined by haematoxylin and eosin staining, AB-PAS staining, and immunohistochemical analysis, and the results demonstrated the formation of cartilage tissue. As a control, all these were not observed in the constructs with Ad5-EGFP transduced hASCs. In conclusion, our study demonstrates that adenovirus-mediated hTGF beta 2 gene transfer is able to induce the hASCs into chondrogenic lineage both ill vitro and in vivo. Ads-hTGF beta 2 transduced hASCs combined with three-dimensional PLGA/alginate compound may be a viable method in treating injuries of cartilage. (c) 2007 Wiley Periodicals, Inc.

语种: 英语
所属项目编号: 30371431 ; 30500254
项目资助者: Chinese National Natural Science Foundation
WOS记录号: WOS:000258637100026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65577
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Natl Human Genome Ctr, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100871, Peoples R China
3.Fourth Mil Med Univ, Coll Stomatol, Dept Dent, Xian 710032, Peoples R China

Recommended Citation:
Jin, Xiao Bing,Sun, Yong Sheng,Zhang, Ke,et al. Tissue engineered cartilage from hTGF beta 2 transduced human adipose derived stem cells seeded in PLGA/alginate compound in vitro and in vivo[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2008,86A(4):1077-1087.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Jin, Xiao Bing]'s Articles
[Sun, Yong Sheng]'s Articles
[Zhang, Ke]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Jin, Xiao Bing]‘s Articles
[Sun, Yong Sheng]‘s Articles
[Zhang, Ke]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace