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学科主题: 临床医学
题名:
Tissue engineered cartilage from hTGF beta 2 transduced human adipose derived stem cells seeded in PLGA/alginate compound in vitro and in vivo
作者: Jin, Xiao Bing1; Sun, Yong Sheng1; Zhang, Ke1; Wang, Jing2; Shi, Tai Ping3; Ju, Xiao Dong1; Lou, Si Quan1
关键词: human adipose derived stem cells (hASCs) ; transforming growth factor beta 2 (TGF beta 2) ; adenovirus ; cartilage ; tissue engineering
刊名: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
发表日期: 2008-09-15
DOI: 10.1002/jbm.a.31656
卷: 86A, 期:4, 页:1077-1087
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: ALGINATE-CHONDROCYTE CONSTRUCTS ; MESENCHYMAL PROGENITOR CELLS ; MARROW STROMAL CELLS ; BONE-MARROW ; ARTICULAR-CARTILAGE ; GENE-EXPRESSION ; CHONDROGENIC DIFFERENTIATION ; FIBER STRUCTURES ; HUMAN FAT ; CULTURE
英文摘要:

Chondrogenic potential of human adipose derived stem cells (hASCs) makes them a possible source of seeding cells for cartilage tissue engineering. We aim to examine the chondrogenic differentiation of human transforming growth factor beta 2 (hTGF beta 2) transduced hASCs seeded in three-dimensional scaffold ill vitro and ill vivo. In this study, hASCs were isolated from human subcutaneous adipose tissue and transduced with a replication deficient adenovirus carrying hTGF beta 2 (Ad5-hTGF beta 2), and then the transduced cells were seeded and cultured in PLGA/alginate compounds. RT-PCR analysis revealed that Ad5-hTGF beta 2 transduced hASCs produced aggrecan and collagen type II after 7-day induction ill vitro and continued throughout the culture period; this was also demonstrated by the positive staining of Alcian blue and immunohistochemistry for collagen type It. For ill vivo study, Ad5-hTGF beta 2 transduced hASCs seeded in PLGA/alginate compounds were implanted in subcutaneous pockets of nude mice; after 12 weeks, the implants were harvested and examined by haematoxylin and eosin staining, AB-PAS staining, and immunohistochemical analysis, and the results demonstrated the formation of cartilage tissue. As a control, all these were not observed in the constructs with Ad5-EGFP transduced hASCs. In conclusion, our study demonstrates that adenovirus-mediated hTGF beta 2 gene transfer is able to induce the hASCs into chondrogenic lineage both ill vitro and in vivo. Ads-hTGF beta 2 transduced hASCs combined with three-dimensional PLGA/alginate compound may be a viable method in treating injuries of cartilage. (c) 2007 Wiley Periodicals, Inc.

语种: 英语
所属项目编号: 30371431 ; 30500254
项目资助者: Chinese National Natural Science Foundation
WOS记录号: WOS:000258637100026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65577
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: 1.Chinese Natl Human Genome Ctr, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100871, Peoples R China
3.Fourth Mil Med Univ, Coll Stomatol, Dept Dent, Xian 710032, Peoples R China

Recommended Citation:
Jin, Xiao Bing,Sun, Yong Sheng,Zhang, Ke,et al. Tissue engineered cartilage from hTGF beta 2 transduced human adipose derived stem cells seeded in PLGA/alginate compound in vitro and in vivo[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2008,86A(4):1077-1087.
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