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学科主题: 药学
题名:
Furanodiene, a Natural Product, Inhibits Breast Cancer Growth Both in vitro and in vivo
作者: Zhong, Zhangfeng1,2; Dang, Yuanye1,2; Yuan, Xia3; Guo, Wei; Li, Yingbo1,2; Tan, Wen1,2; Cui, Jingrong3; Lu, Jinjian1,2; Zhang, Qingwen1,2; Chen, Xiuping1,2; Wang, Yitao1,2
关键词: Furanodiene ; Breast cancer ; Proliferation ; Cell cycle ; Apoptosis
刊名: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
发表日期: 2012
DOI: 10.1159/000341457
卷: 30, 期:3, 页:778-790
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Physiology
研究领域[WOS]: Cell Biology ; Physiology
关键词[WOS]: CELL-CYCLE ARREST ; PRESSURIZED-LIQUID-EXTRACTION ; TRADITIONAL CHINESE MEDICINE ; D-GALACTOSAMINE/LIPOPOLYSACCHARIDE ; ZEDOARIAE RHIZOMA ; CURCUMA RHIZOMES ; LIVER-INJURY ; APOPTOSIS ; ACTIVATION ; SESQUITERPENES
英文摘要:

Purpose: Previous studies have reported that the Curcuma wenyujin Y.H. Chen et C. Ling extract, which has a high furanodiene content, showed anti-cancer effects in breast cancer cells in vitro. The present study was designed to evaluate the in vitro and in vivo anti-cancer activity of furanodiene. Methods: The in vitro effects of furanodiene were examined on two human breast cancer cell lines, MCF-7 and MDA-MB-231 cells. Assays of proliferation, LDH release, mitochondrial membrane potential (Delta Psi m), cell cycle distribution, apoptosis and relevant signaling pathways were performed. The in vivo effect was determined with MCF-7 tumor xenograft model in nude mice. Results: Furanodiene significantly inhibited the proliferation and increased the LDH release in both cell lines in a dose-dependent manner. Delta Psi m depolarization, chromatin condensation, and DNA fragmentation were also observed after furanodiene treatment. Furanodiene dose-dependently induced cell cycle arrest at the G0/G1 phase. The protein expressions of p-cyclin D1, total cyclin D1, p-CDK2, total CDK2, p-Rb, total Rb, Bcl-xL, and Akt were significantly inhibited by furanodiene, whereas the protein expressions of Bad and Bax, and the proteolytic cleavage of caspase-9, caspase-7, and poly-ADP-ribose polymerase (PARP) were dramatically increased. Furthermore, the z-VAD-fmk markedly reversed the furanodiene-induced cell cytotoxicity, the proteolytic cleavage of caspase-9, and DNA fragmentation but did not affect the proteolytic cleavage of PARP, whereas the Akt inhibitor VIII increased the furanodiene-induced cytotoxicity and PARP cleavage. In addition, furanodiene dose-dependently suppressed the tumor growth in vivo, achieving 32% and 54% inhibition rates after intraperitoneal injection of 15 mg/kg and 30 mg/kg, respectively. Conclusions: Taken together, we concluded that furanodiene suppresses breast cancer cell growth both in vitro and in vivo and could be a new lead compound for breast cancer chemotherapy. Copyright (C) 2012 S. Karger AG, Basel

语种: 英语
所属项目编号: 045/2011/A ; 077/2011/A3 ; UL016/09-Y4/CMS/WYT01/ICMS ; MYRG208(Y2-L4)-ICMS11-WYT
项目资助者: Macao Science and Technology Development Fund ; University of Macau
WOS记录号: WOS:000307533600025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65581
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Univ Macau, Inst Chinese Med Sci, Macao, Peoples R China
2.Univ Macau, State Key Lab Qual Res Chinese Med, Macao, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China

Recommended Citation:
Zhong, Zhangfeng,Dang, Yuanye,Yuan, Xia,et al. Furanodiene, a Natural Product, Inhibits Breast Cancer Growth Both in vitro and in vivo[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2012,30(3):778-790.
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