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Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract
Tanaka, Hiroyuki1; Morinaga, Osamu2; Uto, Takuhiro2; Fuji, Shunsuke3; Aboagye, Frederick Asare4; Nguyen Huu Tung2; Li, Xiao Wei5; Putalun, Waraporn6; Shoyama, Yukihiro2
刊名INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY
2012
DOI10.1155/2012/260425
收录类别SCI
文章类型Review
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]IONIZATION MASS-SPECTROMETRY ; TRADITIONAL CHINESE MEDICINES ; IMMUNOAFFINITY COLUMN CHROMATOGRAPHY ; MAJOR GINSENG COMPONENT ; SERUM-ALBUMIN CONJUGATE ; NITRIC-OXIDE ; ENZYME-IMMUNOASSAY ; HERBAL MEDICINES ; PANAX-GINSENG ; GLYCYRRHIZIN
英文摘要

Matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry was used for the confirmation of hapten number in synthesized antigen. As application of MAb, the MAbs against ginsenosides and glycyrrhizin have been prepared resulting in the development of two new techniques that we named the eastern blotting method and the knockout extract preparation. In eastern blotting technique, glycosides like ginsenosides and glycyrrhizin separated by silica gel TLC were blotted to PVDF membrane that was treated with a NaIO4 solution followed by BSA resulted in glycoside-BSA conjugate on a PVDF membrane. The blotted spots were stained by MAb. Double staining of eastern blotting for ginsenosides using antiginsenoside Rb-1 and Rg(1) MAbs promoted complete identification of ginsenosides in Panax species. The immunoaffinity concentration of glycyrrhizin was determined by immunoaffinity column conjugated with antiglycyrrhizin MAb resulting in the glycyrrhizin-knockout extract, which was determined by the synergic effect with glycyrrhizin on NO production using the cell line.

语种英语
WOS记录号WOS:000323431400001
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65602
专题北京大学药学院
作者单位1.Kyushu Univ, Grad Sch Pharmaceut Sci, Higashi Ku, Fukuoka 8120855, Japan
2.Nagasaki Int Univ, Fac Pharmaceut Sci, Sasebo 8593298, Japan
3.Nagasaki Int Univ, Fac Hlth Management, Sasebo 8593298, Japan
4.Univ Ghana, Phytochem Dept, Ctr Sci Res Plant Med, Mampong Akuapem, Ghana
5.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
6.Khon Kaen Univ, Fac Pharmaceut Sci, Khon Kaen 40002, Thailand
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GB/T 7714
Tanaka, Hiroyuki,Morinaga, Osamu,Uto, Takuhiro,et al. Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract[J]. INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY,2012.
APA Tanaka, Hiroyuki.,Morinaga, Osamu.,Uto, Takuhiro.,Fuji, Shunsuke.,Aboagye, Frederick Asare.,...&Shoyama, Yukihiro.(2012).Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract.INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY.
MLA Tanaka, Hiroyuki,et al."Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract".INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY (2012).
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