IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Intratumoral injection of dendritic cells in combination with local hyperthermia induces systemic antitumor effect in patients with advanced melanoma
Guo, Jun; Zhu, Jun; Sheng, Xinan; Wang, Xiaopei; Qu, Li; Han, Yan; Liu, Yuexiang; Zhang, Hui; Huo, Ling; Zhang, Shuhui; Lin, Baohe; Yang, Zhi
关键词dendritic cell hyperthermia melanoma chemokine heat shock protein Treg antitumor immunity
刊名INTERNATIONAL JOURNAL OF CANCER
2007-06-01
DOI10.1002/ijc.22551
120期:11页:2418-2425
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]HEAT-SHOCK PROTEINS ; HEAT-SHOCK-PROTEIN-70 PEPTIDE COMPLEXES ; CHEMOKINE RECEPTOR EXPRESSION ; REGULATORY T-CELLS ; IN-VITRO ; ANTIGEN PRESENTATION ; MALIGNANT-MELANOMA ; RANDOMIZED-TRIAL ; TUMOR-CELLS ; IMMUNOTHERAPY
英文摘要

Dendritic cells (DC) are potent antigen-presenting cells that can present tumor antigens chaperoned by heat shock proteins (HSPs), while local hyperthermia (LHT) can increase the expression of HSPs. In this study, we determine if intratumoral injection of immature DC after LHT (LHT+IT-DC) induces systemic antitumor immunity in patients with advanced melanoma, and investigate the potential immunological mechanisms involved in the treatments. Patients were randomly assigned to intratumoral administration of autologous immature DC triweekly, with (LHT+IT-DC, arm A, n = 9) or without (IT-DC, arm B, n = 9) LHT. Our results showed that there were no grade 3/4 toxicities. The time to progress (TTP) of arm A was 5 months, significantly longer than that in arm B (2 months, p < 0.05). However, the overall survival time had no statistical difference (13 months vs. 6 months, p > 0.05) between the 2 groups. Our ELISPOT assay showed a significantly increased melanoma-specific IFN-gamma production in arm A, suggesting that LHT+IT-DC was more effective in the induction of cytotoxic T lymphocytes (CTL) than IT-DC alone. Furthermore, we detected an increased HSPs expression 4 hr after the first LHT, an enhanced Th1/Th2 chemokines production 24 hr after the first LHT+IT-DC treatment, a promoted migration of DC to afferent lymph nodes, and a decreased infiltration of regulatory T cells (CD4(+)CD25(+)) and an increased infiltration of active CTL (CD8(+)CD28(+)) 48 hr after the third DC injection in arm A patients. Therefore, LHT+IT-DC can induce effective specific antitumor immunity and facilitate a Th1-polarized immune response in patients with advanced melanoma. (c) 2007 Wiley-Liss, Inc.

语种英语
WOS记录号WOS:000245684100018
Citation statistics
Cited Times:40[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65605
Collection北京大学临床肿瘤学院
作者单位1.Peking Univ, Beijing Canc Hosp, Sch Oncol, Dept Renal Canc & Melanoma, Beijing 100036, Peoples R China
2.Peking Univ, Beijing Canc Hosp, Sch Oncol, Dept Centralab, Beijing 100036, Peoples R China
3.Peking Univ, Beijing Canc Hosp, Sch Oncol, Dept Ultrasound, Beijing 100036, Peoples R China
4.Peking Univ, Beijing Canc Hosp, Sch Oncol, Dept Radiotherapy & Hyperthermia, Beijing 100036, Peoples R China
5.Peking Univ, Beijing Canc Hosp, Sch Oncol, Dept Isotope, Beijing 100036, Peoples R China
Recommended Citation
GB/T 7714
Guo, Jun,Zhu, Jun,Sheng, Xinan,et al. Intratumoral injection of dendritic cells in combination with local hyperthermia induces systemic antitumor effect in patients with advanced melanoma[J]. INTERNATIONAL JOURNAL OF CANCER,2007,120(11):2418-2425.
APA Guo, Jun.,Zhu, Jun.,Sheng, Xinan.,Wang, Xiaopei.,Qu, Li.,...&Yang, Zhi.(2007).Intratumoral injection of dendritic cells in combination with local hyperthermia induces systemic antitumor effect in patients with advanced melanoma.INTERNATIONAL JOURNAL OF CANCER,120(11),2418-2425.
MLA Guo, Jun,et al."Intratumoral injection of dendritic cells in combination with local hyperthermia induces systemic antitumor effect in patients with advanced melanoma".INTERNATIONAL JOURNAL OF CANCER 120.11(2007):2418-2425.
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