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学科主题: 药学
题名:
Lanthanide ions promote the hydrolysis of 2,3-bisphosphoglycerate
作者: Zhu, B; Xue, DP; Wang, K
关键词: lanthanide ions ; 2,3-bisphosphoglycerate ; phosphomonoester hydrolysis ; P-31 NMR
刊名: BIOMETALS
发表日期: 2004-08-01
卷: 17, 期:4, 页:423-433
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: NUCLEAR-MAGNETIC-RESONANCE ; HUMAN RED-CELL ; OXYGEN-AFFINITY ; DNA HYDROLYSIS ; METABOLIC DYNAMICS ; ADULT HEMOGLOBIN ; METAL-ION ; 2,3-DIPHOSPHOGLYCERATE ; CERIUM(IV) ; COMPLEXES
英文摘要:

The P-31 NMR studies showed that lanthanide ions promote the site-specific hydrolysis of 2,3-Bisphosphoglycerate (BPG) at pH 7.4 by cleaving the 2′ phosphomonoester bond. The effect of fourteen trivalent lanthanide ions and Sc3+, and Y3+ were compared by the percentage of hydrolysis obtained by determining the inorganic phosphate produced. All the trivalent lanthanide ions promote the hydrolysis, but Sc3+ not. Among them, Ce3+ affects the reaction mostly. This was mainly attributed to the autooxidation of Ce3+ to Ce4+, since the promoting effect of Ce3+ is related to the increasing Ce4+ amount in the solution and depressed by adding sulphite. Ce4+ promotes the hydrolysis more efficiently than Ce3+ do. The pseudo first-order rate constant for the hydrolysis of BPG by Ce(SO4)(2) (18.7 mM) at pH 1 and pH 2, 37degreesC is 3.1 h(-1) and 0.65 h(-1) respectively. A mechanism with a hydroxo species as reactive intermediate was proposed for the trivalent lanthanide ions. The site-specificity was explainable by this mechanism.

语种: 英语
WOS记录号: WOS:000221687900009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65606
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Inst J Paoli I Calmettes, INSERM, U119, F-13009 Marseille, France
2.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China

Recommended Citation:
Zhu, B,Xue, DP,Wang, K. Lanthanide ions promote the hydrolysis of 2,3-bisphosphoglycerate[J]. BIOMETALS,2004,17(4):423-433.
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