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学科主题: 药学
题名:
Study on the interaction between HIV reverse transcriptase and its non-nucleoside inhibitor nevirapine by capillary electrophoresis
作者: Chen, Wenjing1; Li, Wujie1; Ling, Xiaomei1,2; Wang, Xiaowei1; Liu, Junyi1
关键词: HIV reverse transcriptase ; Nevirapine ; Binding constant ; Capillary electrophoresis
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2010-06-15
DOI: 10.1016/j.jchromb.2010.04.013
卷: 878, 期:20, 页:1714-1717
收录类别: SCI ; ISTP
文章类型: Proceedings Paper
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: ZONE-ELECTROPHORESIS ; BINDING-SITE ; DNA ; RESISTANCE
英文摘要:

The HIV reverse transcriptase (RT) is an important antiviral target for the chemotherapy of AIDS because of its key role in virus replication Nevirapine is a first generation of non-nucleoside reverse transcriptase inhibitors (NNRTIs), which is usually used for the therapy of AIDS In this study, a high-performance analytical method based on capillary electrophoresis (CE) to investigate interactions between HIV RT and nevirapine was developed Samples containing HIV RT and nevirapine at various ratios were incubated at 37 degrees C for 45 min and then separated by CE with Tris-acetate buffer at pH 7 3 containing 0 15% SDS Both qualitative and quantitative characterizations of the binding were determined by CE for the first time the binding constants of the interactions between HIV RT and nevirapine were calculated as (3 25 +/- 0.16) x 10(4) and (1 25 +/- 0 07) x 10(2) M(-1) by Scatchard analysis HIV fa and nevirapine have two binding sites The presented methodology should be generally applicable to study the interactions between HIV RT and nevirapine quantitatively and qualitatively (C) 2010 Elsevier B.V All rights reserved

语种: 英语
WOS记录号: WOS:000279134600011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65616
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Chen, Wenjing,Li, Wujie,Ling, Xiaomei,et al. Study on the interaction between HIV reverse transcriptase and its non-nucleoside inhibitor nevirapine by capillary electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2010,878(20):1714-1717.
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