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Study on the interaction between HIV reverse transcriptase and its non-nucleoside inhibitor nevirapine by capillary electrophoresis
Chen, Wenjing1; Li, Wujie1; Ling, Xiaomei1,2; Wang, Xiaowei1; Liu, Junyi1
关键词HIV reverse transcriptase Nevirapine Binding constant Capillary electrophoresis
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2010-06-15
DOI10.1016/j.jchromb.2010.04.013
878期:20页:1714-1717
收录类别SCI ; ISTP
文章类型Proceedings Paper
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]ZONE-ELECTROPHORESIS ; BINDING-SITE ; DNA ; RESISTANCE
英文摘要

The HIV reverse transcriptase (RT) is an important antiviral target for the chemotherapy of AIDS because of its key role in virus replication Nevirapine is a first generation of non-nucleoside reverse transcriptase inhibitors (NNRTIs), which is usually used for the therapy of AIDS In this study, a high-performance analytical method based on capillary electrophoresis (CE) to investigate interactions between HIV RT and nevirapine was developed Samples containing HIV RT and nevirapine at various ratios were incubated at 37 degrees C for 45 min and then separated by CE with Tris-acetate buffer at pH 7 3 containing 0 15% SDS Both qualitative and quantitative characterizations of the binding were determined by CE for the first time the binding constants of the interactions between HIV RT and nevirapine were calculated as (3 25 +/- 0.16) x 10(4) and (1 25 +/- 0 07) x 10(2) M(-1) by Scatchard analysis HIV fa and nevirapine have two binding sites The presented methodology should be generally applicable to study the interactions between HIV RT and nevirapine quantitatively and qualitatively (C) 2010 Elsevier B.V All rights reserved

语种英语
WOS记录号WOS:000279134600011
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65616
专题北京大学药学院_化学生物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Chen, Wenjing,Li, Wujie,Ling, Xiaomei,et al. Study on the interaction between HIV reverse transcriptase and its non-nucleoside inhibitor nevirapine by capillary electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2010,878(20):1714-1717.
APA Chen, Wenjing,Li, Wujie,Ling, Xiaomei,Wang, Xiaowei,&Liu, Junyi.(2010).Study on the interaction between HIV reverse transcriptase and its non-nucleoside inhibitor nevirapine by capillary electrophoresis.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,878(20),1714-1717.
MLA Chen, Wenjing,et al."Study on the interaction between HIV reverse transcriptase and its non-nucleoside inhibitor nevirapine by capillary electrophoresis".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 878.20(2010):1714-1717.
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