IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Effects of cobalt-60 gamma-radiation on the synthesis of adrenomedullin and endothelin in rat vascular smooth muscle cells
Zhong, GZ; Chen, FR; Bu, DF; Li, JX; Qi, YF; Niu, D; Wang, SH; Pang, YZ; Tang, CS
关键词radiation adrenomedullin endothelin restenosis
刊名HEART AND VESSELS
2003-09-01
DOI10.1007/s00380-003-0709-9
18期:4页:207-212
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems ; Peripheral Vascular Disease
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]GENE-EXPRESSION ; INHIBITION ; RESTENOSIS
英文摘要

We studied the effects of cobalt-60 gamma-radiation on the gene expression and secretion of adrenomedullin (Adm) and endothelin (ET) in cultured rat vascular smooth muscle cells (VSMCs). Rat VSMCs cultured in Dulbecoo′s modified Eagle′s medium containing 10% FBS were radiated with cobalt-60 gamma-radiation at doses of 1, 14, and 25 Gy, respectively. Then the mRNA of Adm and ET in VSMCs were detected by the reverse-transcriptative competitive polymerase chain reaction. Adm and ET levels in rat VSMCs were measured by radioimmunoassay. As compared with that of the control, the secretions of Adm in rat VSMCs radiated at doses of 14 and 25 Gy were increased by 270% (P < 0.05) and 233% (P < 0.05), respectively. The mRNA levels of Adm were increased by 82.4% (P < 0.01) and 101% (P < 0.01), respectively. Meanwhile, the secretions of ET were decreased by 27.3% (P < 0.01) and 58.0% (P < 0.01) in VSMCs radiated at doses of 14 and 25 Gy, respectively. In parallel, the mRNA levels of ET were decreased by 47.1% (P < 0.01) and 40.2% (P < 0.01), respectively. Radiation at a dose of 1 Gy had no significant effect on Adm and ET at the gene and protein levels. As compared with the control, the Adm/ET ratios in VSMCs increased by 65% (P > 0.05), 409% (P < 0.01), and 693% (P < 0.01), respectively, with radiation at doses of 1,14 and 25 Gy, respectively. The balance of Adm/ET in VSMCs could be changed by cobalt-60 gamma-radiation, which might play an important role in the use of radiotherapy for restenosis.

语种英语
WOS记录号WOS:000185649600009
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65617
Collection北京大学第三临床医学院_心血管内科
作者单位1.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100083, Peoples R China
2.Peking Univ, Inst Cardiovasc Dis Res, Hosp 1, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Cardiovasc Res Inst, Dept Physiol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Zhong, GZ,Chen, FR,Bu, DF,et al. Effects of cobalt-60 gamma-radiation on the synthesis of adrenomedullin and endothelin in rat vascular smooth muscle cells[J]. HEART AND VESSELS,2003,18(4):207-212.
APA Zhong, GZ.,Chen, FR.,Bu, DF.,Li, JX.,Qi, YF.,...&Tang, CS.(2003).Effects of cobalt-60 gamma-radiation on the synthesis of adrenomedullin and endothelin in rat vascular smooth muscle cells.HEART AND VESSELS,18(4),207-212.
MLA Zhong, GZ,et al."Effects of cobalt-60 gamma-radiation on the synthesis of adrenomedullin and endothelin in rat vascular smooth muscle cells".HEART AND VESSELS 18.4(2003):207-212.
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