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学科主题临床医学
MDA-9 and GRP78 as potential diagnostic biomarkers for early detection of melanoma metastasis
Guan, Ming1; Chen, Xiaofan2,3; Ma, Yingyu4; Tang, Lihua5,6; Guan, Lei7; Ren, Xuefeng8; Yu, Bo3,5,9; Zhang, Wei2,3,9; Su, Bing2,3,8,9
关键词Melanoma MDA-9 GRP78 Exosomes Two-dimensional gel electrophoresis
刊名TUMOR BIOLOGY
2015-04-01
DOI10.1007/s13277-014-2930-9
36期:4页:2973-2982
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]CELL-DERIVED EXOSOMES ; PROTEIN GRP78 ; CANCER-CELLS ; MDA-9/SYNTENIN ; GROWTH ; LYMPHOCYTES ; PROGRESSION ; ACTIVATION ; EXPRESSION ; SURVIVAL
英文摘要

Metastatic melanoma, the primary cause of skin cancer-related death, warrants new diagnostic and therapeutic approaches that target the regulatory machinery at molecular level. The heterogeneity and complexity of melanoma result in the difficulty to find biomarkers and targets for early detection and treatment. Here, we investigated metastasis-associated proteins by comparing the proteomic profiles of primary cutaneous melanomas to their matched lymph node metastases, which minimizes heterogeneity among samples from different patients. Results of two-dimensional gel electrophoresis (2-DE) followed by proteomic analysis revealed eight differentially expressed proteins. Among them, seven proteins (alpha-enolase, cofilin-1, LDH, m-beta-actin, Nm23, GRP78, and MDA-9) showed increased and one (annexin A2) showed decreased expression in metastatic lymph node tissues than in primary melanomas. MDA-9 and GRP78 were the most highly expressed proteins in lymph node metastases, which was validated by immunohistochemical staining. Moreover, exosomes from serum samples of metastatic melanoma patients contained higher levels of MDA-9 and GRP78 than those of patients without metastases, indicating the potential of MDA-9 and GRP78 to be biomarkers for early detection of metastasis. Further, small interfering RNA (siRNA)-mediated knockdown confirmed a functional role for MDA-9 and GRP78 to promote cell invasion in the A375 cells. Finally, we showed that GRP78 co-localized with MDA-9 in 293T cells. Taken together, our findings support MDA-9, co-expressed with GRP78, as a melanoma protein associated with lymph node metastasis. Investigating how MDA-9 and GRP78 interact to contribute to melanoma metastasis and disease progression could reveal new potential avenues of targeted therapy and/or useful biomarkers for diagnosis and prognosis.

语种英语
WOS记录号WOS:000352885900087
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65629
专题北京大学深圳医院_皮肤性病科
作者单位1.Roswell Pk Canc Inst, Dept Pharmacol & Therapeut, Buffalo, NY 14263 USA
2.Fudan Univ, Shanghai Med Coll, Huashan Hosp, Dept Lab Med, Shanghai 200040, Peoples R China
3.Shenzhen Peking Univ, Biomed Res Inst, Shenzhen 518036, Guangdong, Peoples R China
4.Shenzhen Peking Univ, Biomed Res Inst, Shenzhen Key Lab Translat Med Dermatol, Shenzhen 518036, Guangdong, Peoples R China
5.Peking Univ, Shenzhen Hosp, Dept Dermatol, Shenzhen 518036, Guangdong, Peoples R China
6.Peoples Liberat Army, Hosp 303, Dept Dermatol, Nanning 530021, Guangxi Zhuang, Peoples R China
7.Skin Res Ctr Guangzhou Landproof, Guangzhou 510630, Guangdong, Peoples R China
8.SUNY Buffalo, Sch Publ Hlth & Hlth Profess, Dept Epidemiol & Environm Hlth, Buffalo, NY 14214 USA
9.Peking Univ, Shenzhen Hosp, Shenzhen Key Discipline Dermatol, Shenzhen 518036, Guangdong, Peoples R China
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Guan, Ming,Chen, Xiaofan,Ma, Yingyu,et al. MDA-9 and GRP78 as potential diagnostic biomarkers for early detection of melanoma metastasis[J]. TUMOR BIOLOGY,2015,36(4):2973-2982.
APA Guan, Ming.,Chen, Xiaofan.,Ma, Yingyu.,Tang, Lihua.,Guan, Lei.,...&Su, Bing.(2015).MDA-9 and GRP78 as potential diagnostic biomarkers for early detection of melanoma metastasis.TUMOR BIOLOGY,36(4),2973-2982.
MLA Guan, Ming,et al."MDA-9 and GRP78 as potential diagnostic biomarkers for early detection of melanoma metastasis".TUMOR BIOLOGY 36.4(2015):2973-2982.
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