IR@PKUHSC  > 北京大学基础医学院  > 心血管所
学科主题基础医学
C-type natriuretic peptide inhibiting vascular calcification might involve decreasing bone morphogenic protein 2 and osteopontin levels
Chen, Jing-Jing1,2; Zhang, Jing3; Cai, Yan4; Zhou, Ye-Bo4; Wen, Ge-Bo1; Tang, Chao-Shu4; Qi, Yong-Fen4; Jiang, Zhi-Sheng1
关键词C-type natriuretic peptide Vascular calcification Vascular smooth muscle cells
刊名MOLECULAR AND CELLULAR BIOCHEMISTRY
2014-07-01
DOI10.1007/s11010-014-2019-1
392期:1-2页:65-76
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]SMOOTH-MUSCLE-CELLS ; MATRIX GLA PROTEIN ; VITAMIN-D-3 PLUS NICOTINE ; HORMONE-RELATED PEPTIDE ; IN-VITRO ; ARTERIAL CALCIFICATION ; CARDIOVASCULAR-DISEASE ; OSTEOBLASTIC CELLS ; DOWN-REGULATION ; DIFFERENTIATION
英文摘要

Vascular calcification (VC) is highly associated with increased morbidity and mortality in patients with advanced chronic kidney disease. Paracrine/autocrine factors such as vasoactive peptides are involved in VC development. Here, we investigated the expression of the novel peptide C-type natriuretic peptide (CNP) in the vasculature, tested its ability to prevent VC in vivo and in vitro, and examined the mechanism involved. Rat aortic VC was induced by vitamin D3 plus nicotine (VDN). CNP (500 ng/kg/h) was administered by mini-osmotic pump. Calcification was examined by von Kossa staining; CNP and cyclic guanosine monophosphate (cGMP) contents were detected by radioimmunoassay, and mRNA and protein levels were examined by real-time PCR and Western blot analysis in aortas and calcified vascular smooth muscle cells (VSMCs). VDN-treated rat aortas showed higher CNP content and decreased expression of its receptor natriuretic peptide receptor B, along with increased vascular calcium deposition and alkaline phosphatase (ALP) activity. Low CNP levels were accompanied by increased vascular calcium deposition and ALP activity in VDN-treated rats when compared to vehicle treatment, which was further confirmed in cultured VSMCs. Administration of CNP greatly reduced VC in VDN-treated aortas compared with controls, which was confirmed in calcified VSMCs. The decrease in alpha-actin expression was ameliorated by CNP in vitro. Moreover, protein expression levels of osteopontin (OPN) were significantly up-regulated in calcified aortas, and CNP increased OPN expression in calcified aortas. Furthermore, CNP downregulated OPN and bone morphogenic protein 2 (BMP-2) expression in calcified aortas and VSMCs. Modulation of OPN and BMP-2 expression by CNP and the beneficial effects of CNP on calcified VSMCs were blocked significantly by protein kinase G inhibitor H7. Impaired local endogenous CNP and its receptor system may be associated with increased mineralization in vivo in rat aortas with VC, and administration of CNP inhibits VC development in vivo and in vitro, at least in part, via a cGMP/PKG pathway.

语种英语
WOS记录号WOS:000337233900006
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65635
Collection北京大学基础医学院_心血管所
作者单位1.Jinan Fifth Peoples Hosp, Jinan, Shandong, Peoples R China
2.Univ South China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, Hengyang 421001, Hunan, Peoples R China
3.Beijing Normal Univ, Sch PE & Sports Sci, Beijing 100875, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Chen, Jing-Jing,Zhang, Jing,Cai, Yan,et al. C-type natriuretic peptide inhibiting vascular calcification might involve decreasing bone morphogenic protein 2 and osteopontin levels[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2014,392(1-2):65-76.
APA Chen, Jing-Jing.,Zhang, Jing.,Cai, Yan.,Zhou, Ye-Bo.,Wen, Ge-Bo.,...&Jiang, Zhi-Sheng.(2014).C-type natriuretic peptide inhibiting vascular calcification might involve decreasing bone morphogenic protein 2 and osteopontin levels.MOLECULAR AND CELLULAR BIOCHEMISTRY,392(1-2),65-76.
MLA Chen, Jing-Jing,et al."C-type natriuretic peptide inhibiting vascular calcification might involve decreasing bone morphogenic protein 2 and osteopontin levels".MOLECULAR AND CELLULAR BIOCHEMISTRY 392.1-2(2014):65-76.
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