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学科主题: 基础医学
题名:
C-type natriuretic peptide inhibiting vascular calcification might involve decreasing bone morphogenic protein 2 and osteopontin levels
作者: Chen, Jing-Jing1,2; Zhang, Jing3; Cai, Yan4; Zhou, Ye-Bo4; Wen, Ge-Bo1; Tang, Chao-Shu4; Qi, Yong-Fen4; Jiang, Zhi-Sheng1
关键词: C-type natriuretic peptide ; Vascular calcification ; Vascular smooth muscle cells
刊名: MOLECULAR AND CELLULAR BIOCHEMISTRY
发表日期: 2014-07-01
DOI: 10.1007/s11010-014-2019-1
卷: 392, 期:1-2, 页:65-76
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: SMOOTH-MUSCLE-CELLS ; MATRIX GLA PROTEIN ; VITAMIN-D-3 PLUS NICOTINE ; HORMONE-RELATED PEPTIDE ; IN-VITRO ; ARTERIAL CALCIFICATION ; CARDIOVASCULAR-DISEASE ; OSTEOBLASTIC CELLS ; DOWN-REGULATION ; DIFFERENTIATION
英文摘要:

Vascular calcification (VC) is highly associated with increased morbidity and mortality in patients with advanced chronic kidney disease. Paracrine/autocrine factors such as vasoactive peptides are involved in VC development. Here, we investigated the expression of the novel peptide C-type natriuretic peptide (CNP) in the vasculature, tested its ability to prevent VC in vivo and in vitro, and examined the mechanism involved. Rat aortic VC was induced by vitamin D3 plus nicotine (VDN). CNP (500 ng/kg/h) was administered by mini-osmotic pump. Calcification was examined by von Kossa staining; CNP and cyclic guanosine monophosphate (cGMP) contents were detected by radioimmunoassay, and mRNA and protein levels were examined by real-time PCR and Western blot analysis in aortas and calcified vascular smooth muscle cells (VSMCs). VDN-treated rat aortas showed higher CNP content and decreased expression of its receptor natriuretic peptide receptor B, along with increased vascular calcium deposition and alkaline phosphatase (ALP) activity. Low CNP levels were accompanied by increased vascular calcium deposition and ALP activity in VDN-treated rats when compared to vehicle treatment, which was further confirmed in cultured VSMCs. Administration of CNP greatly reduced VC in VDN-treated aortas compared with controls, which was confirmed in calcified VSMCs. The decrease in alpha-actin expression was ameliorated by CNP in vitro. Moreover, protein expression levels of osteopontin (OPN) were significantly up-regulated in calcified aortas, and CNP increased OPN expression in calcified aortas. Furthermore, CNP downregulated OPN and bone morphogenic protein 2 (BMP-2) expression in calcified aortas and VSMCs. Modulation of OPN and BMP-2 expression by CNP and the beneficial effects of CNP on calcified VSMCs were blocked significantly by protein kinase G inhibitor H7. Impaired local endogenous CNP and its receptor system may be associated with increased mineralization in vivo in rat aortas with VC, and administration of CNP inhibits VC development in vivo and in vitro, at least in part, via a cGMP/PKG pathway.

语种: 英语
所属项目编号: 30570766 ; 30971169 ; 81170277 ; 05A043 ; 2008-244
项目资助者: National Natural Science Foundation of China ; Department of Education of Hunan Province ; Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Human Province ; Ministry of Education, P. R. China ; University of South China
WOS记录号: WOS:000337233900006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65635
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: 1.Jinan Fifth Peoples Hosp, Jinan, Shandong, Peoples R China
2.Univ South China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, Hengyang 421001, Hunan, Peoples R China
3.Beijing Normal Univ, Sch PE & Sports Sci, Beijing 100875, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100871, Peoples R China

Recommended Citation:
Chen, Jing-Jing,Zhang, Jing,Cai, Yan,et al. C-type natriuretic peptide inhibiting vascular calcification might involve decreasing bone morphogenic protein 2 and osteopontin levels[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2014,392(1-2):65-76.
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