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学科主题: 药学
题名:
Targeted Cellular Uptake and siRNA Silencing by Quantum-Dot Nanoparticles Coated with beta-Cyclodextrin Coupled to Amino Acids
作者: Zhao, Mei-Xia2; Li, Jin-Ming2; Du, Lingyan1; Tan, Cai-Ping2; Xia, Qing1; Mao, Zong-Wan1,2; Ji, Liang-Nian2
关键词: cellular uptake ; gene delivery ; nanoparticles ; quantum dots ; siRNA
刊名: CHEMISTRY-A EUROPEAN JOURNAL
发表日期: 2011-04-01
DOI: 10.1002/chem.201003523
卷: 17, 期:18, 页:5171-5179
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: CDSE/CDS CORE/SHELL NANOCRYSTALS ; IN-VIVO ; INTRACELLULAR DELIVERY ; GENE DELIVERY ; LIVING CELLS ; CYTOTOXICITY ; VITRO ; TRANSFECTION ; STABILITY ; RELEASE
英文摘要:

Quantum dots (QDs) have the potential to serve as photostable beacons to track siRNA delivery, which is fast becoming an attractive approach to probe gene function in cells. In this paper, we synthesized QD nanoparticles coated with beta-cyclodextrin (beta-CD) coupled to amino acids with different surface charges (positive, negative, and neutral) through direct ligand-exchange reactions and used them to deliver siRNA. We found that these QDs are diffluent in biological buffer with high colloidal stability and have strong optical emission properties similar to those of tri-n-octylphosphine oxide (TOPO)-coated QDs and also have a long fluorescence lifetime 12.5 ns for L-His-beta-CD-coated CdSe/ZnSe QDs). The results of in vitro cytotoxicity and internalization of these modified QDs in normal and cancer cells showed that the b-CD coupled to amino acid outlayers greatly improved the biocompatibility of QDs, and conferred with lower cytotoxicity even at very high concentration. In particular, the L-His-beta-CD-coated CdSe/ZnSe QDs presented lower cytotoxicity to these cells (CC50 value is 180.6 +/- 3.4 mu gmL(-1) in ECV-304 cells for 48 h). Transmission electron microscope (TEM) images showed that the QDs were localized in vesicles in the cytoplasm of the cells. Furthermore, compared with existing transfection agents, gene-silencing efficiency of the modified QDs was slightly improved for HPV18 E6 gene in HeLa cells by gel electrophoresis analysis. Finally, the unique optical properties of QDs allow visible imaging of siRNA delivery in live cells. Taken together, our study not only provides new insights into the mechanisms of amino acid mediated delivery, but also greatly facilities the monitoring of gene-silencing studies.

语种: 英语
所属项目编号: 20725103 ; 20831006 ; 20821001 ; 20901005 ; 9351027501000003 ; 2007CB815306
项目资助者: National Natural Science Foundation of China ; Guangdong Provincial Natural Science Foundation ; National Basic Research Program of China (973 Program) ; Fundamental Research Funds for the Central Universities
WOS记录号: WOS:000290213100029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65639
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, State Key Lab Nat & Biomimet Drug, Beijing 100191, Peoples R China
2.Sun Yat Sen Univ, Sch Chem & Chem Engn, MOE Key Lab Bioinorgan & Synthet Chem, Guangzhou 510275, Guangdong, Peoples R China

Recommended Citation:
Zhao, Mei-Xia,Li, Jin-Ming,Du, Lingyan,et al. Targeted Cellular Uptake and siRNA Silencing by Quantum-Dot Nanoparticles Coated with beta-Cyclodextrin Coupled to Amino Acids[J]. CHEMISTRY-A EUROPEAN JOURNAL,2011,17(18):5171-5179.
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