IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
The telomerase activity and expression of hTERT gene can serve as indicators in the anti-cancer treatment of human ovarian cancer
Sun, Peng-Ming; Wei, Li-Hui; Luo, Mei-Yu; Liu, Guang; Wang, Jiang-Liu; Mustea, Alexander; Koensgen, Dorninique; Lichtenegger, Werner; Sehouli, Jaild
关键词ovarian cancers telomearase activity hTERT mRNA biomarker apoptosis
刊名EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY
2007-02-01
DOI10.1016/j.ejogrb.2006.01.028
130期:2页:249-257
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]CELL-LINES ; CHEMOSENSITIVITY ; CARCINOMA ; IDENTIFICATION ; CHEMOTHERAPY ; EXTENSION ; SUBUNITS ; BENIGN
英文摘要

Objective: The aim of this study was to evaluate the clinicopahtological significance of telomerase activity and expression of hTERT gene in human ovarian cancer. The potential value of them as indicators for chemotherapy in ovarian cancer cells was also studied.

Materials and methods: A total of 73 samples and ovarian cancer cell lines HO-8910 and COC1 were studied. Telomerase activity was detected by PCR-TRAP-ELISA assay and the expression of the hTERT mRNA was analyzed by semi-quatititative RT-PCR. Alteration of the telomerase activity and hTERT mRNA were also analyzed in the ovarian cancer cells treated with different concentration and different time of cisplatin. Cytogenetic analysis was performed to compare the telomere status in the OH-8910 cells pre- and post-cisplatin treatment. The associations between these two markers and cisplatin induced-apoptosis were respectively analyzed in COC1 cells by the flow cytometry.

Results: Telomerase activity are highly increased in malignancy (0.795 +/- 0.168(A450-655 nm)) than borderline (0.389 +/- 0.174(A450-655) nm), benign tumors (0.236 +/- 0.102(A450-655) (nm)) and normal ovary (0.213 +/- 0.070(A450-655 nm)) (p < 0.05). Twenty samples showed detectable levels of hTERT. The hTERT gene positive lesion showed significantly higher telomerase activity than negative (p = 0.004). There is a significant correlation between the telomerase activity and expression of hTERT (r = 0.921). Both telomerase activity and expression of hTERT can reflect the chemotherapeutic effect of cisplatin in a time-dependent and dose-dependent manner. Treatment with 10 mu M cisplatin, the hTERT mRNA decreased after 12 h and completely disappeared after 48 h, whereas the telomerase activity did not decrease until 24 h. Results front cytogenetic analysis and flow cytometry assay confirmed that the alterations of these two markers are associated with the anticancer treatment of cisplatin.

Conclusion: Expression of hTERT gene is rate-limiting with the activation of telomerase. Both of they may be useful in the predicting of chemotherapeutic effect in ovarian cancer. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000244283600018
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65652
专题北京大学第二临床医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Peking Univ Peoples Hosp, Dept Obstet & Gynecol, Beijing 100044, Peoples R China
2.Charite Univ Med Berlin, Dept Obstet & Gynecol, D-13353 Berlin, Germany
3.Fujian Provincial Maternal & Children Hlth Hosp, Dept Gynecol, Fuzhou 350001, fujian, Peoples R China
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GB/T 7714
Sun, Peng-Ming,Wei, Li-Hui,Luo, Mei-Yu,et al. The telomerase activity and expression of hTERT gene can serve as indicators in the anti-cancer treatment of human ovarian cancer[J]. EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY,2007,130(2):249-257.
APA Sun, Peng-Ming.,Wei, Li-Hui.,Luo, Mei-Yu.,Liu, Guang.,Wang, Jiang-Liu.,...&Sehouli, Jaild.(2007).The telomerase activity and expression of hTERT gene can serve as indicators in the anti-cancer treatment of human ovarian cancer.EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY,130(2),249-257.
MLA Sun, Peng-Ming,et al."The telomerase activity and expression of hTERT gene can serve as indicators in the anti-cancer treatment of human ovarian cancer".EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY 130.2(2007):249-257.
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