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Cluster characterization of mouse embryonic stem cell-derived pluripotent embryoid bodies in four distinct developmental stages
Qin, J.1,2; Guo, X.1; Cui, G. H.1,2; Zhou, Y. C.1; Zhou, D. R.1; Tang, A. R.1; Yu, Z. D.1; Gui, Y. T.1; Cai, Z. M.1
关键词Embryonic stem cells Embryoid body Differentiation
刊名BIOLOGICALS
2009-08-01
DOI10.1016/j.biologicals.2009.03.001
37期:4页:235-244
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Pharmacology & Pharmacy
资助者Natural Science Funds of Guangdong Province ; Medical &amp ; Science Funds of Guangdong Province ; "985" foundation ; Natural Science Funds of Guangdong Province ; Medical &amp ; Science Funds of Guangdong Province ; "985" foundation
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Pharmacology & Pharmacy
关键词[WOS]IN-VITRO DIFFERENTIATION ; GERM-CELLS ; GENE-EXPRESSION ; BODY ; LINEAGES ; DERIVATION ; SYSTEM ; MODEL ; KIT
英文摘要

The formation of embryoid bodies (EBs) is the principal step in the differentiation of embryonic stem (ES) cells. In this study, the morphological characteristics and gene expression patterns of EBs related to the sequential stages of embryonic development were well defined in four distinct developmental groups over 112 days of culture: early-stage EBs groups (1-7 days of differentiation), mid-stage EBs groups (9-15 days of differentiation), maturing EBs groups (17-45 days of differentiation) and matured EBs groups (50 days of differentiation). We first determined definite histological location of apoptosis within EBs and the sequential expression of molecular markers representing stem cells (Oct4, SSEA-1, Sox-2 and AKP), germ cells (Fragilis, Dazl, c-kit, StellaR, Mvh and Stra8), ectoderm (Neurod, Nestin and Neurofilament), mesoderm (Gata-1, Flk-1 and Hbb) and endoderm (AFP and Transthyretin). Our results revealed that developing EBs possess either pluripotent stem cell or germ cell states and that three-dimensional aggregates of EBs initiate mES cell differentiation during prolonged culture in vitro. Therefore, we suggest that this EB system to some extent recapitulates the early developmental processes occurring in vivo. (C) 2009 The International Association for Biologicals. Published by Elsevier Ltd. All fights reserved.

语种英语
所属项目编号7300931 ; 132007158 ; 985-2-054-29
资助者Natural Science Funds of Guangdong Province ; Medical &amp ; Science Funds of Guangdong Province ; "985" foundation ; Natural Science Funds of Guangdong Province ; Medical &amp ; Science Funds of Guangdong Province ; "985" foundation
WOS记录号WOS:000269936400006
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65657
专题北京大学深圳医院
作者单位1.Peking Univ, Shenzhen Hosp, Key Lab Male Reprod & Genet Guangdong Prov, Shenzhen 518036, Peoples R China
2.Med Ctr PKU HKUST, Shenzhen 518036, Peoples R China
推荐引用方式
GB/T 7714
Qin, J.,Guo, X.,Cui, G. H.,et al. Cluster characterization of mouse embryonic stem cell-derived pluripotent embryoid bodies in four distinct developmental stages[J]. BIOLOGICALS,2009,37(4):235-244.
APA Qin, J..,Guo, X..,Cui, G. H..,Zhou, Y. C..,Zhou, D. R..,...&Cai, Z. M..(2009).Cluster characterization of mouse embryonic stem cell-derived pluripotent embryoid bodies in four distinct developmental stages.BIOLOGICALS,37(4),235-244.
MLA Qin, J.,et al."Cluster characterization of mouse embryonic stem cell-derived pluripotent embryoid bodies in four distinct developmental stages".BIOLOGICALS 37.4(2009):235-244.
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