IR@PKUHSC  > 北京大学基础医学院  > 医学信息学系
学科主题医学信息学
Systematic identification of Class I HDAC substrates
Li, Tingting1; Song, Boyan2; Wu, Zheng1; Lu, Ming1; Zhu, Wei-Guo2
关键词Class I HDACs deacetylation prediction sequence feature specificity
刊名BRIEFINGS IN BIOINFORMATICS
2014-11-01
DOI10.1093/bib/bbt060
15期:6页:963-972
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Mathematical & Computational Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Mathematical & Computational Biology
关键词[WOS]HUMAN HISTONE DEACETYLASE ; SUPPORT VECTOR MACHINES ; LARGE GENE LISTS ; LYSINE ACETYLATION ; TRANSCRIPTION FACTORS ; BETA ENHANCEOSOME ; SIRT1 ; CLONING ; PHOSPHORYLATION ; PGC-1-ALPHA
英文摘要

Lysine acetylation is a common post-translational modification of histone and non-histone proteins. This process has an important function in regulating transcriptional activities and other biological processes. Although several computer programs have been developed to predict protein acetylation sites, deacetylases responsible for known or predicted acetylation sites remain unknown. In this research, Class I histone deacetylases (HDACs) substrates were manually obtained, and sequence features of deacetylation sites were analyzed. We found that three members of Class I HDACs (HDAC1, HDAC2 and HDAC3) shared similar sequence features. Therefore, a method was proposed to identify the substrates of Class I HDACs. We evaluated the efficiency of the prediction based on P-value distribution analysis and leave-one-out test. To validate the result of the prediction, we overexpressed Class I HDACs in cells and detected the acetylation levels of potential substrates. In the experiment, five of the seven predicted proteins were deacetylated by Class I HDACs. These results suggested that our method could effectively predict protein deacetylation sites. The work has been integrated to the website ASEB, which was freely available at http://cmbi.bjmu.edu.cn/huac.

语种英语
WOS记录号WOS:000345386100008
项目编号2011CBA01104 ; 2012AA020401 ; 60905014 ; 31030041
资助机构National Basic Research Program of China ; National High-Tech Research and Development Program of China ; National Natural Science Foundation of China
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65670
专题北京大学基础医学院_医学信息学系
北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Biomed Informat, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Li, Tingting,Song, Boyan,Wu, Zheng,et al. Systematic identification of Class I HDAC substrates[J]. BRIEFINGS IN BIOINFORMATICS,2014,15(6):963-972.
APA Li, Tingting,Song, Boyan,Wu, Zheng,Lu, Ming,&Zhu, Wei-Guo.(2014).Systematic identification of Class I HDAC substrates.BRIEFINGS IN BIOINFORMATICS,15(6),963-972.
MLA Li, Tingting,et al."Systematic identification of Class I HDAC substrates".BRIEFINGS IN BIOINFORMATICS 15.6(2014):963-972.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Systematic identific(580KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Tingting]的文章
[Song, Boyan]的文章
[Wu, Zheng]的文章
百度学术
百度学术中相似的文章
[Li, Tingting]的文章
[Song, Boyan]的文章
[Wu, Zheng]的文章
必应学术
必应学术中相似的文章
[Li, Tingting]的文章
[Song, Boyan]的文章
[Wu, Zheng]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Systematic identification of Class I HDAC substrates.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。