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学科主题: 临床医学
题名:
Modification of the RpoS network with a synthetic small RNA
作者: Jin, Ye1,2; Wu, Jianting3,4; Li, Yannan2; Cai, Zhiming4; Huang, Jian-Dong1
刊名: NUCLEIC ACIDS RESEARCH
发表日期: 2013-09-01
DOI: 10.1093/nar/gkt604
卷: 41, 期:17, 页:8332-8340
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: ESCHERICHIA-COLI HFQ ; SM-LIKE PROTEIN ; STATIONARY-PHASE ; MESSENGER-RNA ; SOLUBLE-RNA ; DSRA ; TRANSLATION ; BINDING ; EXPRESSION ; RESISTANCE
英文摘要:

Translation of the sigma factor RpoS is activated by DsrA, RprA and ArcA, three small non-coding sRNAs (sRNA) that expose the ribosome-binding site (RBS) by opening up an inhibitory loop. In the RpoS network, no sRNAs have been found to pair with the RBS, a most common sRNA target site in bacteria. Here, we generate Ribo-0, an artificial sRNA, which represses rpoS translation by pairing with the RBS. Ribo-0 bypasses the RNA chaperon Hfq but requires the RBS to be loosely blocked. Ribo-0 interacts with DsrA and reshapes the RpoS network. Specifically, in the intact RpoS network, DsrA activates rpoS translation by freeing up the RBS. In the modified RpoS network where Ribo-0 is introduced, the DsrA-caused RBS exposure facilitates Ribo-0 binding, thereby strengthening Ribo-0 inhibition. In other words, Ribo-0 changes DsrA from an activator to an accomplice for repressing rpoS translation. This work presents an artificial mechanism of rpoS regulation, reveals mutual effects of native and synthetic players and demonstrates genetic context-dependency of their functions.

语种: 英语
所属项目编号: HKU1/CRF/10 ; 201007176077
项目资助者: Research Grants Council Collaborative Research Fund ; University of Hong Kong
WOS记录号: WOS:000325175900035
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65675
Appears in Collections:北京大学深圳医院_泌尿外科_期刊论文

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作者单位: 1.Univ Hong Kong, Dept Biochem, Li Ka Shing Fac Med, Pok Fu Lam, Hong Kong, Peoples R China
2.Chinese Acad Sci, GIAT HKU Joint Ctr Synthet Biol Engn Res, Guangzhou Inst Adv Technol, Guangzhou, Guangdong, Peoples R China
3.Peking Univ, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol, Shenzhen Hosp,Shenzhen PKU HKUST Med Ctr, Shenzhen, Peoples R China
4.Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Shenzhen 518035, Guangdong, Peoples R China

Recommended Citation:
Jin, Ye,Wu, Jianting,Li, Yannan,et al. Modification of the RpoS network with a synthetic small RNA[J]. NUCLEIC ACIDS RESEARCH,2013,41(17):8332-8340.
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