IR@PKUHSC  > 北京大学深圳医院  > 泌尿外科
学科主题临床医学
Modification of the RpoS network with a synthetic small RNA
Jin, Ye1,2; Wu, Jianting3,4; Li, Yannan2; Cai, Zhiming4; Huang, Jian-Dong1
刊名NUCLEIC ACIDS RESEARCH
2013-09-01
DOI10.1093/nar/gkt604
41期:17页:8332-8340
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]ESCHERICHIA-COLI HFQ ; SM-LIKE PROTEIN ; STATIONARY-PHASE ; MESSENGER-RNA ; SOLUBLE-RNA ; DSRA ; TRANSLATION ; BINDING ; EXPRESSION ; RESISTANCE
英文摘要

Translation of the sigma factor RpoS is activated by DsrA, RprA and ArcA, three small non-coding sRNAs (sRNA) that expose the ribosome-binding site (RBS) by opening up an inhibitory loop. In the RpoS network, no sRNAs have been found to pair with the RBS, a most common sRNA target site in bacteria. Here, we generate Ribo-0, an artificial sRNA, which represses rpoS translation by pairing with the RBS. Ribo-0 bypasses the RNA chaperon Hfq but requires the RBS to be loosely blocked. Ribo-0 interacts with DsrA and reshapes the RpoS network. Specifically, in the intact RpoS network, DsrA activates rpoS translation by freeing up the RBS. In the modified RpoS network where Ribo-0 is introduced, the DsrA-caused RBS exposure facilitates Ribo-0 binding, thereby strengthening Ribo-0 inhibition. In other words, Ribo-0 changes DsrA from an activator to an accomplice for repressing rpoS translation. This work presents an artificial mechanism of rpoS regulation, reveals mutual effects of native and synthetic players and demonstrates genetic context-dependency of their functions.

语种英语
WOS记录号WOS:000325175900035
项目编号HKU1/CRF/10 ; 201007176077
资助机构Research Grants Council Collaborative Research Fund ; University of Hong Kong
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65675
专题北京大学深圳医院_泌尿外科
作者单位1.Univ Hong Kong, Dept Biochem, Li Ka Shing Fac Med, Pok Fu Lam, Hong Kong, Peoples R China
2.Chinese Acad Sci, GIAT HKU Joint Ctr Synthet Biol Engn Res, Guangzhou Inst Adv Technol, Guangzhou, Guangdong, Peoples R China
3.Peking Univ, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol, Shenzhen Hosp,Shenzhen PKU HKUST Med Ctr, Shenzhen, Peoples R China
4.Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Shenzhen 518035, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Jin, Ye,Wu, Jianting,Li, Yannan,et al. Modification of the RpoS network with a synthetic small RNA[J]. NUCLEIC ACIDS RESEARCH,2013,41(17):8332-8340.
APA Jin, Ye,Wu, Jianting,Li, Yannan,Cai, Zhiming,&Huang, Jian-Dong.(2013).Modification of the RpoS network with a synthetic small RNA.NUCLEIC ACIDS RESEARCH,41(17),8332-8340.
MLA Jin, Ye,et al."Modification of the RpoS network with a synthetic small RNA".NUCLEIC ACIDS RESEARCH 41.17(2013):8332-8340.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Jin, Ye]的文章
[Wu, Jianting]的文章
[Li, Yannan]的文章
百度学术
百度学术中相似的文章
[Jin, Ye]的文章
[Wu, Jianting]的文章
[Li, Yannan]的文章
必应学术
必应学术中相似的文章
[Jin, Ye]的文章
[Wu, Jianting]的文章
[Li, Yannan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。