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学科主题: 药学
题名:
Lanthanum Enhances In Vitro Osteoblast Differentiation via Pertussis Toxin-Sensitive Gi Protein and ERK Signaling Pathway
作者: Wang, Xi1; Yuan, Lan2; Huang, Jian1; Zhang, Tian-Lan1; Wang, Kui1
关键词: LA(3+) ; OSTEOBLAST ; DIFFERENTIATION ; GI PROTEIN ; ERK PATHWAY
刊名: JOURNAL OF CELLULAR BIOCHEMISTRY
发表日期: 2008-12-01
DOI: 10.1002/jcb.21932
卷: 105, 期:5, 页:1307-1315
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: CATION-SENSING MECHANISM ; CHRONIC-RENAL-FAILURE ; REGULATED KINASE ERK ; COUPLED RECEPTORS ; PHOSPHATE BINDER ; BONE-CELLS ; PROLIFERATION ; EXPRESSION ; IDENTIFICATION ; ACTIVATION
英文摘要:

Converging lines of evidence suggest that lanthanum tends to deposit in bone. The influence of lanthanum ion (La3+) on osteoblast differentiation and the related mechanism are essential to understanding its effect on bone metabolism. In this study, La3+ treatment enhanced in vitro osteoblast differentiation as evidenced by promoting alkaline phosphatase (ALP) activity, osteocalcin (OC) secretion, and matrix mineralization. The expressions of osteoblast-specific genes of Cbfa-1, osteopontin (OPN), and bone sialoprotein (BSP) were all increased in the presence of La3+, but no change was observed in that of type I collagen (COL-I). Further studies demonstrated that La3+ treatment enhanced phosphorylation of extracellular signal-regulated kinase (ERK). Inhibition of ERK activation by U0126 suppressed the effects of La3+ on osteoblast activity. Moreover, pretreatment of the cells with pertussis toxin (PTx), a Gi protein inhibitor, suppressed the La3+-enhanced ERK phosphorylation and osteoblast differentiation. These findings suggest that La3+ exposure enhances in vitro osteoblast differentiation and the effect depends on ERK phosphorylation via PTx-sensitive Gi protein signaling. J. Cell. Biochem. 105: 1307-1315, 2008. (c) 2008 Wiley-Liss, Inc.

语种: 英语
所属项目编号: 2007CB516806 ; 20571006 ; 20637010 ; 2062007).
项目资助者: National Basic Research Program of China ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing
WOS记录号: WOS:000261539100018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65676
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Peking Univ, Med & Hlth Anal Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Wang, Xi,Yuan, Lan,Huang, Jian,et al. Lanthanum Enhances In Vitro Osteoblast Differentiation via Pertussis Toxin-Sensitive Gi Protein and ERK Signaling Pathway[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2008,105(5):1307-1315.
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