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学科主题: 基础医学
题名:
T-type Ca2+ channel expression in human esophageal carcinomas: A functional role in proliferation
作者: Lu, Fengmin3; Chen, Hairu1,2; Zhou, Chun1,2; Liu, Shuang4; Guo, Mingzhou5; Chen, Pingping6; Zhuang, Hui3; Xie, Dong6; Wu, Songwei1,2
关键词: T-type calcium channel ; esophageal cancer ; proliferation ; p21(CIPI)
刊名: CELL CALCIUM
发表日期: 2008
DOI: 10.1016/j.ceca.2007.03.006
卷: 43, 期:1, 页:49-58
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: CALCIUM-CHANNELS ; CANCER-CELLS ; SKELETAL-MUSCLE ; PRIMARY CULTURE ; CYCLIN D1 ; CURRENTS ; MIBEFRADIL ; GENE ; P53 ; DIFFERENTIATION
英文摘要:

In the present study the role of T-type Ca2+ channels in cancer cell proliferation was examined. Seventeen human esophageal cancer cell lines were screened for T-type channels using RT-PCR and voltage-clamp recordings. mRNAs for all three T-type channel alpha(1)-subunits (alpha(IG), alpha(IH), and alpha(II)) were detected in all 17 cell lines: either alpha(1H) alone, alpha(IH) and alpha(IG), or all three T-type alpha(I)-subunits. Eleven cell lines were further subjected to voltage-clamp recordings: one, i.e. the TE8 cell line, was found to exhibit a typical T-type current while others exhibited a minimal or no T-type current. Cell proliferation assays were performed in the presence or absence of T-type channel blocker mibefradil in KYSE150, KYSE 180 and TE 1 cells expressing mRNA for T-type channel alpha(1)-subunits Eleven cell lines were further subjected to voltage-clamp recordings: one, i.e. the TE8 cell line, was found to exhibit a typical T-type current while others exhibited a minimal or no T-type current. Cell proliferation assays were performed in the presence or absence of T-type channel blocker mibefradil in KYSE150, KYSE180 and TEI cells expressing mRNA for T-type channel alpha(1)-subunits but lacking T-type current, and TE8 cells exhibiting T-type current. Only TE8 cell proliferation was reduced by mibefradil. Silencing the alpha(IG)-gene that encodes functional T-type Ca2+ channels in TE8 cells with type-specific shRNA transduction also significantly decreased TE8 cell proliferation. The reduction of cell proliferation in TE8 cells was found to be associated with an up-regulation of p21(CIPI). Moreover, p53 silencing nearly abolished the up-regulation of p21(CIPI) resulting from mibefradil T-type channel blockade. Together, these findings suggest a functional role of T-type channels in certain esophageal carcinomas, and that inhibition of T-type channels reduces cell proliferation via a p53-dependent p21(CIPI) pathway. (c) 2007 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000253281300006
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65704
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Otsuka Beijing Res Inst, Beijing 100738, Peoples R China
2.Univ S Alabama, Coll Med, Ctr Lung Biol, Mobile, AL 36608 USA
3.Univ S Alabama, Coll Med, Dept Pharmacol, Mobile, AL 36608 USA
4.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100083, Peoples R China
5.Sidney Kimmel Comprehens Canc Ctr Johns Hopkins, Dept Oncol, Baltimore, MD 21231 USA
6.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200031, Peoples R China

Recommended Citation:
Lu, Fengmin,Chen, Hairu,Zhou, Chun,et al. T-type Ca2+ channel expression in human esophageal carcinomas: A functional role in proliferation[J]. CELL CALCIUM,2008,43(1):49-58.
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