IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
T-type Ca2+ channel expression in human esophageal carcinomas: A functional role in proliferation
Lu, Fengmin3; Chen, Hairu1,2; Zhou, Chun1,2; Liu, Shuang4; Guo, Mingzhou5; Chen, Pingping6; Zhuang, Hui3; Xie, Dong6; Wu, Songwei1,2
关键词T-type calcium channel esophageal cancer proliferation p21(CIPI)
刊名CELL CALCIUM
2008
DOI10.1016/j.ceca.2007.03.006
43期:1页:49-58
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]CALCIUM-CHANNELS ; CANCER-CELLS ; SKELETAL-MUSCLE ; PRIMARY CULTURE ; CYCLIN D1 ; CURRENTS ; MIBEFRADIL ; GENE ; P53 ; DIFFERENTIATION
英文摘要

In the present study the role of T-type Ca2+ channels in cancer cell proliferation was examined. Seventeen human esophageal cancer cell lines were screened for T-type channels using RT-PCR and voltage-clamp recordings. mRNAs for all three T-type channel alpha(1)-subunits (alpha(IG), alpha(IH), and alpha(II)) were detected in all 17 cell lines: either alpha(1H) alone, alpha(IH) and alpha(IG), or all three T-type alpha(I)-subunits. Eleven cell lines were further subjected to voltage-clamp recordings: one, i.e. the TE8 cell line, was found to exhibit a typical T-type current while others exhibited a minimal or no T-type current. Cell proliferation assays were performed in the presence or absence of T-type channel blocker mibefradil in KYSE150, KYSE 180 and TE 1 cells expressing mRNA for T-type channel alpha(1)-subunits Eleven cell lines were further subjected to voltage-clamp recordings: one, i.e. the TE8 cell line, was found to exhibit a typical T-type current while others exhibited a minimal or no T-type current. Cell proliferation assays were performed in the presence or absence of T-type channel blocker mibefradil in KYSE150, KYSE180 and TEI cells expressing mRNA for T-type channel alpha(1)-subunits but lacking T-type current, and TE8 cells exhibiting T-type current. Only TE8 cell proliferation was reduced by mibefradil. Silencing the alpha(IG)-gene that encodes functional T-type Ca2+ channels in TE8 cells with type-specific shRNA transduction also significantly decreased TE8 cell proliferation. The reduction of cell proliferation in TE8 cells was found to be associated with an up-regulation of p21(CIPI). Moreover, p53 silencing nearly abolished the up-regulation of p21(CIPI) resulting from mibefradil T-type channel blockade. Together, these findings suggest a functional role of T-type channels in certain esophageal carcinomas, and that inhibition of T-type channels reduces cell proliferation via a p53-dependent p21(CIPI) pathway. (c) 2007 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000253281300006
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被引频次:39[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65704
专题北京大学基础医学院_病原生物学系
作者单位1.Otsuka Beijing Res Inst, Beijing 100738, Peoples R China
2.Univ S Alabama, Coll Med, Ctr Lung Biol, Mobile, AL 36608 USA
3.Univ S Alabama, Coll Med, Dept Pharmacol, Mobile, AL 36608 USA
4.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100083, Peoples R China
5.Sidney Kimmel Comprehens Canc Ctr Johns Hopkins, Dept Oncol, Baltimore, MD 21231 USA
6.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200031, Peoples R China
推荐引用方式
GB/T 7714
Lu, Fengmin,Chen, Hairu,Zhou, Chun,et al. T-type Ca2+ channel expression in human esophageal carcinomas: A functional role in proliferation[J]. CELL CALCIUM,2008,43(1):49-58.
APA Lu, Fengmin.,Chen, Hairu.,Zhou, Chun.,Liu, Shuang.,Guo, Mingzhou.,...&Wu, Songwei.(2008).T-type Ca2+ channel expression in human esophageal carcinomas: A functional role in proliferation.CELL CALCIUM,43(1),49-58.
MLA Lu, Fengmin,et al."T-type Ca2+ channel expression in human esophageal carcinomas: A functional role in proliferation".CELL CALCIUM 43.1(2008):49-58.
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