北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 北京大学肝病研究所  > 期刊论文
学科主题: 临床医学
题名:
DCs Pulsed with Novel HLA-A2-Restricted CTL Epitopes against Hepatitis C Virus Induced a Broadly Reactive Anti-HCV-Specific T Lymphocyte Response
作者: Guo, Zhongsheng1,2; Zhang, Henghui1,2; Rao, Huiying1,2; Jiang, Dong1,2; Cong, Xu1,2; Feng, Bo1,2; Wang, Jianghua1,2; Wei, Lai1,2; Chen, Hongsong1,2
刊名: PLOS ONE
发表日期: 2012-06-12
DOI: 10.1371/journal.pone.0038390
卷: 7, 期:6
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: HEPATOCELLULAR-CARCINOMA PATIENTS ; I CLINICAL-TRIAL ; DENDRITIC CELLS ; THERAPEUTIC VACCINATION ; INFECTED INDIVIDUALS ; IMMUNE-RESPONSES ; PEPTIDE VACCINE ; B-VIRUS ; CYTOKINES ; VITRO
英文摘要:

Objective: To determine the capacity of dendritic cells (DCs) loaded with single or multiple-peptide mixtures of novel hepatitis C virus (HCV) epitopes to stimulate HCV-specific cytotoxic T lymphocyte (CTL) effector functions.

Methods: A bioinformatics approach was used to predict HLA-A2-restricted HCV-specific CTL epitopes, and the predicted peptides identified from this screen were synthesized. Subsequent IFN-gamma ELISPOT analysis detected the stimulating function of these peptides in peripheral blood mononuclear cells (PBMCs) from both chronic and self-limited HCV infected subjects (subjects exhibiting spontaneous HCV clearance). Mature DCs, derived in vitro from CD14(+) monocytes harvested from the study subjects by incubation with appropriate cytokine cocktails, were loaded with novel peptide or epitope peptide mixtures and co-cultured with autologous T lymphocytes. Granzyme B (GrB) and IFN-gamma ELISPOT analysis was used to test for epitope-specific CTL responses. T-cell-derived cytokines contained in the co-cultured supernatant were detected by flow cytometry.

Results: We identified 7 novel HLA-A2-restricted HCV-specific CTL epitopes that increased the frequency of IFN-gamma-producing T cells compared to other epitopes, as assayed by measuring spot forming cells (SFCs). Two epitopes had the strongest stimulating capability in the self-limited subjects, one found in the E2 and one in the NS2 region of HCV; five epitopes had a strong stimulating capacity in both chronic and self-limited HCV infection, but were stronger in the self-limited subjects. They were distributed in E2, NS2, NS3, NS4, and NS5 regions of HCV, respectively. We also found that mDCs loaded with novel peptide mixtures could significantly increase GrB and IFN-gamma SFCs as compared to single peptides, especially in chronic HCV infection subjects. Additionally, we found that DCs pulsed with multiple epitope peptide mixtures induced a Th1-biased immune response.

Conclusions: Seven novel and strongly stimulating HLA-A2-restricted HCV-specific CTL epitopes were identified. Furthermore, DCs loaded with multiple-epitope peptide mixtures induced epitope-specific CTLs responses.

语种: 英语
所属项目编号: 2008ZX10002-012 ; 2008ZX10002-013
项目资助者: National S&amp ; T Major Project for Infectious Diseases Control in China
WOS记录号: WOS:000305340000020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65755
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Inst Hepatol, Peoples Hosp, Beijing 100871, Peoples R China
2.Beijing Key Lab Hepatitis C & Immunotherapy Liver, Beijing, Peoples R China

Recommended Citation:
Guo, Zhongsheng,Zhang, Henghui,Rao, Huiying,et al. DCs Pulsed with Novel HLA-A2-Restricted CTL Epitopes against Hepatitis C Virus Induced a Broadly Reactive Anti-HCV-Specific T Lymphocyte Response[J]. PLOS ONE,2012,7(6).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Guo, Zhongsheng]'s Articles
[Zhang, Henghui]'s Articles
[Rao, Huiying]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Guo, Zhongsheng]‘s Articles
[Zhang, Henghui]‘s Articles
[Rao, Huiying]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace