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Optimization of the extraction conditions and simultaneous quantification by RP-LC of six alkaloids in evodiae fructus
Zhao, Meng-yao; Yang, Xiu-wei
关键词column liquid chromatography quantification orthogonal array design lkaloids evodiae fructus
刊名CHROMATOGRAPHIA
2008-04-01
DOI10.1365/s10337-008-0543-4
67期:7-8页:543-550
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]INDOLOQUINAZOLINE ALKALOIDS ; VANILLOID RECEPTORS ; IN-VITRO ; RUTAECARPA ; DEHYDROEVODIAMINE ; PEPTIDE ; FRUITS ; CELLS ; MICE ; VIVO
英文摘要

A reversed phase liquid chromatographic (LC) method coupled with DAD (250 nm) has been developed and validated for simultaneous quantification of six alkaloids, dehydroevodiamine (1), wuzhuyuamide-I (2), 5-hydroxyrutaecarpine (3), 14-formyldihydrorutaecarpine (4), evodiamine (5) and rutaecarpine (6), in 12 batches evodiae fructus [the dried, unripe fruits of Evodia rutaecarpa (Juss.) Benth. or E. rutaecarpa (Juss.) Benth. var. officinalis (Dode) Huang, E. rutaecarpa (Juss.) Benth. var. bodinieri (Dode) Huang] as a traditional Chinese medicine. The method was carried out by a C-18 column (250 x 4.6 mm) with a gradient mobile phase of methanol, acetonitrile, and phosphoric acid-triethylamine-buffer solution. The contents of 1-6 in the evodiae fructus could easily be determined within 70 min. The experimental results were satisfactory for the intra-day and inter-day precision and accuracy of the method for simultaneous determination. The linear calibration ranges of 1-6 were 40-1,000, 20-500, 1-100, 10-500, 40-1,000 and 80-1,000 mu g mL(-1). The recoveries of 1-6 were 97.43-103.73% with RSDs from 0.21 to 1.99%. The limits of detection for 1-6 were 2.0, 2.0, 0.1, 1.0, 5.0 and 5.0 mu g mL(-1), and the limits of quantification were 6.6, 6.6, 0.3, 3.3, 16.5 and 16.5 mu g mL(-1). The method was successfully applied to the quantification of six alkaloids in the evodiae fructus.

语种英语
WOS记录号WOS:000254750200006
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65756
专题北京大学药学院
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Meng-yao,Yang, Xiu-wei. Optimization of the extraction conditions and simultaneous quantification by RP-LC of six alkaloids in evodiae fructus[J]. CHROMATOGRAPHIA,2008,67(7-8):543-550.
APA Zhao, Meng-yao,&Yang, Xiu-wei.(2008).Optimization of the extraction conditions and simultaneous quantification by RP-LC of six alkaloids in evodiae fructus.CHROMATOGRAPHIA,67(7-8),543-550.
MLA Zhao, Meng-yao,et al."Optimization of the extraction conditions and simultaneous quantification by RP-LC of six alkaloids in evodiae fructus".CHROMATOGRAPHIA 67.7-8(2008):543-550.
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