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学科主题: 临床医学
题名:
Different patterns of agonist-induced modulation of alpha(1B)-adrenoceptor density at different initial expression levels
作者: Lei, BL; Han, QD
关键词: alpha(1B)-adrenoceptor ; human embryonic kidney cells ; regulation ; signal transduction
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2000-09-01
卷: 45, 期:17, 页:1575-1578
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: PROTEIN
英文摘要:

We compared the norepinephrine (NE) induced alpha(1B)-adrenoceptor (alpha(1B)-AR) expression modulation between two transfected human embryonic kidney (HEK) 293 cell lines in which alpha(1B)-AR densities were (6336 +/- 913) and (773 +/- 164) fmol.mg(-1), respectively. Treatment of cells with NE (10 mu mol.L-1) for 48 h decreased high-level expressed alpha(1B)-AR density, but increased low-level expressed alpha(1B)-AR density. The protein kinase C inhibitor Calphostin C or Re-31-8220 reversed, and its activator PMA mimicked the NE-induced down-regulation of high-level expressed alpha(1B)-AR. Moreover, PMA induced a down-regulation of low-level expressed alpha(1B)-AR. The endoplasmic reticulum Ca2+-ATPase inhibitor cyclopiazonic acid (CPA) and the calcium chelator BAPTA/AM did not affect the down-regulation of high-level expressed alpha(1B)-AR, but inhibited the up-regulation of low-level expression alpha(1B)-AR induced by NE. These results suggest that alpha(1B)-adrenoceptor densities at different initial expression levels are differentially regulated by NE and their signal transduction pathways are different.

语种: 英语
WOS记录号: WOS:000089247900007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65770
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: Beijing Med Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China

Recommended Citation:
Lei, BL,Han, QD. Different patterns of agonist-induced modulation of alpha(1B)-adrenoceptor density at different initial expression levels[J]. CHINESE SCIENCE BULLETIN,2000,45(17):1575-1578.
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